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تاثیر حشرهکش بینو 2 و عوامل بیماریزای قارچی بر فعالیت آنزیمهای گوارشی، سم زدا و کیتینازی شته مومی کلم ، Brevicoryne brassicae (Hemiptera: Aphididae) | ||
کنترل بیولوژیک آفات و بیماری های گیاهی | ||
دوره 12، شماره 1، فروردین 1402، صفحه 73-94 اصل مقاله (1.8 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jbioc.2024.375379.342 | ||
نویسندگان | ||
فریبا مهرخو* 1؛ مهدیه موسوی2؛ نورپر گوز3؛ یوبرت قوستا2؛ رمزی آتلیهان4 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ارومیه، ارومیه، ایران | ||
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه ارومیه ارومیه، ایران | ||
3پژوهشکده زیست فناوری، دانشگاه آنکارا، آنکارا، ترکیه | ||
4گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه یوزینجی یل، وان، ترکیه. | ||
چکیده | ||
شته مومی کلم Brevicoryne brassicae (L.)، یکی از مهمترین آفات محصولات کلم میباشد. استفاده از حشرهکشهایی با منشا گیاهی و عوامل کنترل بیولوژیک نظیر قارچهای بیماریزای حشرات به عنوان روشهای تکمیلی در مدیریت تلفیقی آفت مورد توجه قرار گرفته است. در این پژوهش، تاثیر حشرهکش بینو 2 و دو گونه قارچ بیماریزای حشرات، Metarhizium anisopliae AB و Lecanicillium lecanii 229، بهعنوان حشرهکشهای بیولوژیک، روی محتوای پروتئینی، فعالیت پروتئازهای گوارشی، آنزیم کیتیناز، آنزیم های استیلکولین استراز و گلوتاتیون-اس-ترانسفراز حشره کامل شته مومی کلم در دمای 2±25 درجه سلسیوس، رطوبت نسبی 5±60 درصد و دوره نوری 16 ساعت روشنایی و 8 ساعت تاریکی بررسی شد. نتایج نشان داد که استفاده از غلظت کشنده (LC50) ایزولههای قارچی با افزایش ترشح آنزیمهای پروتئازی و کیتینازی باعث هضم پروتئین و کیتین موجود در کوتیکول شته شده و حساسیت شتهها را نسبت به این عوامل افزایش دادند. همچنین حشرهکش بینو2 با کاهش میزان پروتئین و مهار فعالیت آنزیمهای گوارشی، اثر ضدتغذیهای داشته است. به موازات کاهش فعالیت آنزیم های گوارشی، فعالیت آنزیمهای سمزدا هم تحت تاثیر حشرهکش بینو2 کاهش یافت. نتایج این تحقیق نشان داد که قارچهای مورد مطالعه و حشرهکش بینو2 منجر به افزایش مرگ و میر حشرات کامل شته مومی کلم شدند و از اطلاعات حاصله میتوان در مدیریت این آفت استفاده کرد. | ||
کلیدواژهها | ||
حشره کش با منشا گیاهی؛ قارچهای بیماریزای حشرات؛ شته مومی کلم؛ پروتئازهای گوارشی؛ آنزیم های سم زدا | ||
مراجع | ||
اصغرنژاد، شیوا (1399). مقایسهی اثر زیر کشندگی دو سم بینو2 و فلونیکامید بر فراسنجههای دموگرافی شته مومی کلم Brevicoryne brassicae (Hem.:Aphididae) در شرایط آزمایشگاهی. پایاننامه کارشناسی ارشد. بهراهنمایی فریبا مهرخو. ارومیه: دانشگاه ارومیه، دانشکدة کشاورزی. حسینی نوه، وحید و قدمیاری، محمد (1392). مبانی و مفاهیم روشهای آزمایشگاهی در بیوشیمی، فیزیولوژی و سمشناسی حشرات. چاپ اول. تهران: موسسه انتشارات دانشگاه تهران. خانجانی، محمد ( 1384). آفات گیاهان زراعی ایران. انتشارات دانشگاه بوعلی سینا. همدان. چاپ اول. 467 صفحه. خرمی، فرشته (1397). ارزیابی کارایی عصارههای متانولی گیاهان زنیان و رازیانه با سه گونه متفاوت از قارچهای بیماریزای حشرات با راهکارهای نانوفرمولاسیون جهت کنترل بید سیبزمینی. رساله دکتری. به راهنمایی فریبا مهرخو. ارومیه: دانشگاه ارومیه، دانشکدة کشاورزی. رشادت سلوانق، نازی (1400). بررسی اثرات زیرکشندگی فلونیکامید و بینو2 روی فراسنجههای رشد جمعیت Trialeurodes vaporariorum (Hemiptera: Aleyrodidae).. پایاننامه کارشناسی ارشد. بهراهنمایی فریبا مهرخو. ارومیه: دانشگاه ارومیه، دانشکدة کشاورزی. موسوی، مهدیه. 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