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تغییرات فصلی میزان آگار و دو هورمون گیاهی در ماکرو جلبک Gracilaria corticata (Rhodophyta) | ||
شیلات | ||
دوره 77، شماره 1، فروردین 1403، صفحه 53-68 اصل مقاله (1.26 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jfisheries.2024.367216.1408 | ||
نویسندگان | ||
حدیقه صائب مهر* 1؛ فرناز رفیعی2؛ محمدهادی گیویان راد3؛ گلاله مصطفوی4 | ||
1دانشآموخته دکتری، گروه فناوریهای زیستی، دانشکدة منابع طبیعی و محیطزیست، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران | ||
2استادیار گروه بیولوژی دریا، واحد تهران شمال، دانشگاه آزاد اسلامی، تهران، ایران | ||
3استادیار گروه شیمی، واحد علوم و تحقیقات تهران، دانشگاه آزاد اسلامی، تهران، ایران | ||
4استادیار،گروه زیستشناسی، واحد یادگار امام خمینی (ره) شهرری، دانشگاه آزاد اسلامی، تهران، ایران | ||
چکیده | ||
جلبکهای دریایی قرمز حاوی ترکیباتی مانند آگار و هورمونهای تنظیمکنندة رشد هستند که در صنایع مصارف متعددی دارند. این تحقیق جهت تعیین تودة زندة و استخراج دو هورمون و آگار از جلبک Gracilaria corticata انجام شد. نمونهبرداری از دی 1394 تا آبان 1395 در بندر بوشهر یک ماه در میان انجام گرفت. تودة زنده و پارامترهای محیطی در ترانسکت انتخابی اندازهگیری و جلبک برداشت شد. پس از مراحل استخراج، با روشHPLC هورمونها جداسازی و توسط تزریق استاندارد شناسایی شدند. بیشترین میزان آگار در تیر ماه 63/3 % ، زآتین در شهریور ماه 21/90% و ایندول بوتیریک اسید 21/19% در دیماه بود. بیشترین تودة زندة این جلبک در اسفند ماه 423/33 گرم در مترمربع اندازهگیری شد. نتایج حاصل از آزمون آماری ANOVA وChi-squared تفاوت معنیداری را در 6 ماه نمونهبرداری در تمامی نمونهها نشان داد (0/05>P). در بین عوامل محیطی آگار با شوری همبستگی داشت. ایندول بوتیریک اسید در دی ماه باعث رشد زیاد جلبک شد اما زآتین تأثیر معنیداری بر رشد این گونه نشان نداد. بهطور کلی میتوان بهمنظور استخراج این مواد، در ماههایی که بیشترین مقدار را داشتهاند برداشت صورت گیرد. با توجه به قیمت گزاف این هورمونها و ضرورت استفاده از آنها در عصارههای جلبکی و کودمایع جلبکی، این تحقیق شروعی برای استخراج انواع هورمونها از این جلبکها است. | ||
کلیدواژهها | ||
آگار؛ استخراج؛ تنظیمکنندههای رشد؛ گراسیلاریا | ||
مراجع | ||
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