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فعالیت ضداکسایشی فراکسیون‌های به‌دست آمده از فراپالایش غشایی فوکوئیدان هیدرولیزشدة جلبک قهوه ای Nizamuddinia zanardinii | ||
| شیلات | ||
| دوره 76، شماره 2، خرداد 1402، صفحه 195-207 اصل مقاله (1.14 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jfisheries.2022.345894.1338 | ||
| نویسندگان | ||
| نفیسه سادات موسوی1؛ مهدی طبرسا* 2؛ حسن احمدی گاولیقی3 | ||
| 1دانشجوی کارشناسی ارشد، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران | ||
| 2دانشیار، گروه فرآوری محصولات شیلاتی، دانشکده علوم دریایی، دانشگاه تربیت مدرس، نور، ایران | ||
| 3دانشیار، گروه صنایع غذایی، دانشکده کشاورزی، دانشگاه تربیت مدرس، نور، ایران | ||
| چکیده | ||
| پلی ساکاریدها از جمله درشت مولکول‌های زیستی هستند که علاوه بر ویژگی های فیزیکو-شیمیایی منحصر به فرد، توانایی بروز خواص زیست‌فعالی امیدوارکننده ای با توجه به ساختار شیمیایی خود دارند. هدف از مطالعة حاضر، ارزیابی تأثیر وزن مولکولی تولیدی با استفاده از سیستم فراپالایش غشایی بر ویژگی های ضداکسایشی فوکوئیدان هیدرولیزشده جلبک قهوهای Nizamuddinia zanardinii بود. پس از حذف رنگدانه ها و ترکیبات با وزن مولکولی پایین، فوکوئیدانخام استخراج شده در دمای ۱۰۰ درجة سانتی گراد به مدت ۲۰ دقیقه (FH20) توسط 0/1 نرمال اسید هیدروکلریک تحت هیدرولیز قرارگرفت. سپس FH20 با استفاده از سیستم فراپالایش غشایی با غشای ۲، ۱۰، ۳۰، ۱۰۰ کیلودالتون فراکسیون‌گیری شد. میانگین وزن مولکولی فراکسیون ها به ترتیب 533/7، 39/2، 12/4 و 4/6 ×۱۰3 گرم/مول در KDa >100،KDa 30-100، KDa 10-30 و KDa 2-10 بود. پس از تفکیک براساس وزن مولکولی، فراکسیون های به‌دست آمده نسبت به FH20، به میزان قابل توجهی قادر به مهار رادیکال آزاد DPPH(37/69-05/85 درصد)، رادیکال آزاد کاتیونی ABTS (55/43-65/89 درصد) و احیاء یون Fe3+ (جذب 0/07-0/72) بود. در ایـن میـان، فراکسیون KDa ۲-۱۰ بیشترین پتانسیل را در مهار رادیکال آزاد DPPH (74/12-85/05 درصد)، رادیکال آزاد کاتیونی ABTS (75/16-89/65 درصد) و احیاء یون آهن Fe3+ (جذب 0/21- 0/72) دارا بود. به طورکلی، نتایج این مطالعه نشان داد تغییر وزن مولکولی فوکوئیدان می تواند سبب افزایش قابل توجه فعالیت ضداکسایشی شود. | ||
| کلیدواژه‌ها | ||
| فراپالایش؛ وزن مولکولی؛ ضداکسایشی؛ ترکیبات غذا دارو؛ Nizamuddinia zanardinii؛ هیدرولیز | ||
| عنوان مقاله [English] | ||
| Antioxidant activity of purified fractions obtained from membrane ultrafiltration of hydrolysed fucoidan from brown seaweed Nizamuddinia zanardinii | ||
| نویسندگان [English] | ||
| Nafiseh Sadat Mousavi1؛ Mehdi Tabarsa2؛ Hassan Ahmadi Gavlighi3 | ||
| 1M.Sc. Student, Department of Seafood Processing, Tarbiat Modares University, Nur, Iran | ||
| 2Associate Professor, Department of Seafood Processing, Tarbiat Modares University, Nur, Iran | ||
| 3Associate Professor, Department of Food Science and Technology, Tarbiat Modares University, Tehran, Iran | ||
| چکیده [English] | ||
| Polysaccharides are one of the biomacromolecules that, in addition to their peculiar physicochemical properties, possess promising biological capabilities depend on chemical structure. The purpose of the present study was to evaluate the effect of membrane ultrafiltration on antioxidant properties of hydrolysed fucoidan from brown seaweed Nizamuddinia zanardinii. After removing pigments and low molecular weight compounds, the extracted crude fucoidan, was hydrolysed by 0.01N hydrochloric acid at 100°C for 20 minutes (FH20). Then, FH20 was fractionated using ultrafiltration with 2, 10, 30, 100 kDa membranes. The average molecular weight of fractions was 553.7, 39.2, 12.4 and 4.6×103 g/mol in KDa>100, 100-30 KDa, 30-10 KDa and 2-10 KDa, respectively. After separation based on molecular weight, the obtained fractions, compared to FH20, which were significantly capable of inhibiting DPPH free radicals (85.05-37.69%), ABTS radicals (89.65-55.43%) and reduction Fe3+ ions (absorption of 0.72-0.07). In the meantime, 2-10 KDa fraction had the greatest potential in inhibiting DPPH free radicals (85.05-74.12%), ABTS free radicals (89.65-75.16%) and reducing Fe3+ ions (absorption of 0.21-0.72). Overall, the results of this study showed that reducing the molecular weight of fucoidan to a specific range could lead to significant increase of antioxidant activity. | ||
| کلیدواژه‌ها [English] | ||
| Ultrafiltration, Molecular weight, Antioxidant, Nutraceuticals, Nizamuddinia zanardinii, Hydrolysis | ||
| مراجع | ||
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