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استخراج فایکوسیانین ازجلبک Arthrospira platensis با استفاده از حلالها و روشهای مختلف | ||
شیلات | ||
دوره 75، شماره 2 - شماره پیاپی 4، تیر 1401، صفحه 183-192 اصل مقاله (987.86 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jfisheries.2021.333458.1292 | ||
نویسندگان | ||
فهیمه شاه حسینی1؛ محمدعلی نعمت اللهی* 2؛ سیدولی حسینی3 | ||
1دانش آموخته کارشناسی ارشد گروه شیلات، پردیس کشاورزی و منابع طبیعی، دانشگاه تهران | ||
2استاد گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
3دانشیار گروه شیلات، دانشکده منابع طبیعی، دانشگاه تهران، ایران | ||
چکیده | ||
در این مطالعه، استخراج رنگدانه فایکوسیانین از میکروجلبک Arthrospira platensis با استفاده از حلالها (آب مقطر، بافر سدیم فسفات) و روشهای مختلف (سونیکیشن، مایکروویو و انجماد و ذوب) برای مقایسه و تعیین بهترین حلال و روش به منظور رسیدن به بیشترین بازده انجام شد. میزان 1/0 گرم پودر جلبک A. platensis در 10 میلی لیترحلال (آب مقطر و بافر سدیم فسفات (1/0 ± 7.4 pH=)( در سه روش استخراج سونیکیشن با دامنه 50درصد به مدت 15 دقیقه، مایکروویو در 100 وات به مدت 3 دقیقه و انجماد در 2 ± 20- درجه سانتی گراد به مدت 24 ساعت و ذوب در دمای اتاق انجام شد. تجزیه واریانس برای میانگین دادهها± SD (انحراف معیار) با استفاده ازSPSS در سطح اطمینان (05/0 P <) به دست آمد. بیشترین بازده استخراج توسط بافر سدیم فسفات و روش انجماد و ذوب (mg/g 55/1 ± 3/146PC = ) و کمترین توسط آب مقطر و روش مایکروویو ( mg/g68/2 ± 4/94 PC =) به دست آمد. | ||
کلیدواژهها | ||
اسپیرولینا؛ فایکوسیانین؛ استخراج؛ سونیکیشن؛ مایکروویو | ||
مراجع | ||
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