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TaqMan real-time PCR: a reliable method to detect meat species | ||
| Journal of Food and Bioprocess Engineering | ||
| دوره 3، شماره 2، اسفند 2020، صفحه 116-120 اصل مقاله (484.09 K) | ||
| نوع مقاله: Original research | ||
| شناسه دیجیتال (DOI): 10.22059/jfabe.2020.310078.1065 | ||
| نویسندگان | ||
| Sahar Porzahmat Shirvan1؛ Maryam Azizkhani* 1؛ Maryam Torabi2؛ Mahyar Sharifan3 | ||
| 1Department of Food Hygiene, Faculty of Veterinary Medicine, Amol University of Special Modern Technologies, Amol, Iran | ||
| 2Central Laboratory of Veterinary Head Office of Khorasan Razavi Province, Iran | ||
| 3Central Laboratory of Veterinary Head Office of North Khorasan Province, Iran | ||
| چکیده | ||
| The aim of this study was to detect and identify meat species (beef, lamb, pork, chicken, donkey, and horse) in Kebab as a Halal meat-based food sold in on-road-restaurants of North Khorasan province (Iran) using TaqMan real-time polymerase chain reaction (PCR). Raw Kebab samples (150 samples) were obtained from on-road restaurants. The samples were prepared, DNA was extracted and TaqMan real-time PCR using beef, lamb, pork, chicken, horse, and donkey target genes was performed. The results indicated that 100% of samples yielded positive results of beef, but none of them generated donkey and pork amplicons. The samples of Kebab indicated 93.33% lamb, 83.33% chicken, 10% horse positive results. The information claimed for the source of meat was not correct for more than 95% of the samples and only one food center labeled the Kebab mix correctly. Except for one Kebab sample that showed a positive result for the presence of horse meat, no sample contained meat of species considered illegal in Halal foods. | ||
| کلیدواژهها | ||
| Kebab؛ Polymerase chain reaction؛ Species detection؛ TaqMan probe | ||
| مراجع | ||
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