Pourbabaei, A. A., Khoshhal Nakhjiri, E., Torabi, E., Farahbakhsh, M.. (1399). Dissipation of butachlor by a new strain of Pseudomonas sp. isolated from paddy soils. , 6(3), 627-635. doi: 10.22059/poll.2020.299245.762
A. A. Pourbabaei; E. Khoshhal Nakhjiri; E. Torabi; M. Farahbakhsh. "Dissipation of butachlor by a new strain of Pseudomonas sp. isolated from paddy soils". , 6, 3, 1399, 627-635. doi: 10.22059/poll.2020.299245.762
Pourbabaei, A. A., Khoshhal Nakhjiri, E., Torabi, E., Farahbakhsh, M.. (1399). 'Dissipation of butachlor by a new strain of Pseudomonas sp. isolated from paddy soils', , 6(3), pp. 627-635. doi: 10.22059/poll.2020.299245.762
Pourbabaei, A. A., Khoshhal Nakhjiri, E., Torabi, E., Farahbakhsh, M.. Dissipation of butachlor by a new strain of Pseudomonas sp. isolated from paddy soils. , 1399; 6(3): 627-635. doi: 10.22059/poll.2020.299245.762

Dissipation of butachlor by a new strain of Pseudomonas sp. isolated from paddy soils

Pollution
دوره 6، شماره 3، پاییز 2020، صفحه 627-635    اصل مقاله (552.32 K)
نوع مقاله: Original Research Paper
شناسه دیجیتال (DOI): 10.22059/poll.2020.299245.762
نویسندگان
A. A. Pourbabaei* 1؛ E. Khoshhal Nakhjiri1؛ E. Torabi2؛ M. Farahbakhsh1
1Department of Soil Science, Faculty of Agricultural Engineering and Technology, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
2Department of Plant Protection, Faculty of Agricultural Science and Engineering, College of Agriculture and Natural Resources, University of Tehran, Karaj, Iran
چکیده
Butachlor (BUT) is a chloroacetanilide herbicide widely applied to rice paddies to control annual grass and broad-leaf weeds. A BUT-degrading bacterial strain (PK) was isolated from paddy soils. Biochemical and 16S rRNA sequencing characteristics confirmed the strain as Pseudomonas aeruginosa (99% resemblance). The isolate dissipated BUT (100 μg/mL) in an M9 liquid medium with a rate of 0.5 ± 0.03 day-1 and DT50 and DT90 of 1.38 ± 0.10 days and 4.58 ± 0.32 days, respectively. Soil dissipation of BUT was investigated under flooded conditions. In sterile soils, the isolate increased the dissipation of BUT (200 μg/g) (DT50 = 12.38 ± 1.83 days, DT90 = 41.12 ± 6.09 days, k = 0.06 ± 0.01 day-1) compared to sterile non-inoculated samples (DT50 = 26.87 ± 2.82 days, DT90 = 89.25 ± 9.36 days, k = 0.03 ± 0.00 day-1). In non-inoculated non-sterile soil experiments, the dissipation of BUT was faster (DT50 = 15.17 ± 2.11 days, DT90 = 50.38 ± 7.02 days, k = 0.05 ± 0.00 day-1) compared to non-inoculated sterile ones, and inoculating the isolate accelerated the removal of BUT in non-sterile soils significantly (DT50 = 8.03 ± 1.20 days, DT90 = 26.68 ± 3.97 days, k = 0.09 ± 0.01 day-1). BUT inhibited soil respiration (SR) initially for 5 days, followed by an increase until day 20. The increase in SR was more pronounced in the co-presence of BUT and the isolate. The results of this research suggest P. aeruginosa PK as a suitable candidate for BUT bioremediation.
کلیدواژه‌ها
Bacterial isolate؛ Bioremediation؛ Pseudomonas aeruginosa؛ Paddy؛ Soil respiration
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