بررسی پتانسیل استفاده از چند محیط کشت‌ طبیعی مایع برای تولید بلاستوسپور‌‌‌‌های دو قارچ بیمارگر حشراتMetarhizium anisopliae و Beauveria bassiana

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Balakrishnan, S., Srikanth, J., Santhalakshmi, G., Hari, K. & Sankaranarayanan, C. (2011). Response of the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae to molasses media fortified with supplements. Journal of Sugarcane Research, 1(2), 57-65.

Bena-Molaei, P., Talaei-Hassanloui, R., Askary, H. & Kharazi-Pakdel, A. (2009). Study on potential of some solid natural substances in production of Beauveria bassiana (Ascomycota: Cordycipitaceae) conidia. Journal of Entomological Society of Iran, 30 (2), 1-15. (In Persian).    

 Bena-Molaei, P., Talaei-Hassanloui, R. & Askary, H. (2011). Effect of culture substrates on virulence of Beauveria bassiana (Ascomycota: Cordycipitaceae) conidia against the browntail moth, Euproctis chrysorrhoea (Lep.: Lymantriidae). Journal of Biocontrol Science and Technology, 21(5), 619- 624.

Bigham, Z. and Talaei-Hassanloui, R. 2017. Effect of Some Liquid and Solid Media on Sporulation of Beauveria bassiana. Biological Control pf Pests and Plant Diseases 6 (1): 103-109 (In Farsi).

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Kamp, A. M. & Bidochka, M. J. (2002). Conidium production by insect pathogenic fungi on commercially available agar. Journal of Letters in Applied Microbiology, 35, 74-77.    

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Kassa, A., Brownbridge, M., Parker, B. L., Skinner, M., Gouli, V., Gouli, S., Gou, M., Lee, F. & Hata, T. (2008). Whey for mass production of Beauveria bassiana and Metarhizium anisopliae. Journal of Mycological Research, 112 (5), 583-591.                                                                                                                                                                            

Kim, J. S., Skinner, M. & Parker, B. L. (2010). Influence of whey permeate and millet as substrates on thermotolerance of Beauveria bassiana and Metarhizium anisopliae conidia during storage. Journal of Biocontrol Science and Technology, 20(8), 859-863.                                                                                                                           

Lacey, L. A., Kirk, A. A., Millar, L., Mercadier, G. & Vidal, C. (1999). Ovicidal and larvicidal activity of conidia and blastospores of Paecilomyces fumosoroseus (Deuteromycotina: hyphomycetes) against Bemisia argentifolii (Homoptera: Aleyrodidae) with a description of a bioassay system allowing prolonged survival of control insects. Journal of Biocontrol Science and Technology, 9, 9-18. 

Li, D. P. & Holdom, D. G. (1995). Effects of nutrients on colony formation, growth, and sporulation of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes). Journal of Invertebrate Pathology, 65, 253–260.تعریف‌های Beauveria bassiana (Balsamo) Vuellemin and Metarhizium anisopliae (Metschnikoff) Sorokin are entomopathogenic fungi (EPF) that occur naturally in the environment and have a potential as biological control agents against many insect pests. Different solid substrates i.e such as grains, vegetable wastes, maize, bran, cotton seed, rice husk, wheat and liquid media such as coconut water were evaluated at variable moisture content and yeast extract concentration ffor mass production of two entomopathogenic fungi: Beauveria bassiana (Bals.) Vuellemin and Metarhizium anisopliae. In the present study rice is used as a best solid substrate for spore production and their viability but fungus also grows equally well on maize or other grains. This gives an overview of the mass production of fungal spores using diphasic liquid-solid fermentation i.e LUBILOSA system which is particularly well adapted to the production of EPF’s B. bassiana and M. anisoliae and provides explanations for the use of various techniques and procedures.                                                                                                      

Ottati-de-Lima, E. L., Batista Filho, A., Almeida, J. E., Gassen, M. H., Wenzel, I. M., Almeida, A. M. & Zapellini, L. O.  (2014). Liquid production of entomopathogenic fungi and ultraviolet radiation and temperature effects on produced propagules. Journal of Arquivos do Instituto Biológico, São Paulo, 81(4), 342- 350.                                                                                                                                                                                   

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Prasad, C. S. & Pal, R. (2014). Mass production and economics of entomopathogenic fungus, Beauveria bassiana, Metarhizium anisopliae and Verticillium lecanii on agricultural and industrial waste. Scholars Journal of Agriculture and Veterinary Sciences, 1(1), 28-32.                                                                                                                                 

Rayati, D. J., Nyoman, I., Aryantha, P.  & Arbianto, P. (2001). The optimization of nutrient factors in spore production of Paecilomyces fumosoroseus (Wize) Brown & Smith with submerged – surface fermentation system.The Fifth Symposium on Agri-Bioche, March-11-2001. Tokyo, Japan.

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Singh, R., Verma, K. D., Singh, J., Nigam, R., Singh, M. & Kumar, A. (2017). Multiplication of entomopathogenic fungus Beauberia bassiana (Balsamo) on solid and liquid media. Journal of Pharmacognosy and Phytochemistry,

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Smith, R. J. & Grula, E. A.  (1981). Nutritional requirments for conidial germination and hyphal growth of Beauveria bassiana. Journal of Invertebrate Pathology, 37,222-230. 

Sujatha, K. Srilakshmi, C. & Lenin, E. A. (2016). Selection of a suitable medium for the mass production of some selected entamopathogenic fungus. International Journal of Recent Scientific Research, 7(7),12370-12372.                                                                

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