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بررسی خصوصیات ضدمیکروبی برخی سویه‌های Streptomyces دریایی بر علیه سه بیمارگر گیاهی | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 7، دوره 6، شماره 1، خرداد 1396، صفحه 73-82 اصل مقاله (535.75 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2017.206254.153 | ||
| نویسندگان | ||
| مهسا شمس1؛ بهار شهنواز* 2 | ||
| 1دانشجوی سابق کارشناسی ارشد، گروه زیست‌شناسی، دانشکده علوم، دانشگاه فردوسی مشهد | ||
| 2استادیار، گروه زیست‌شناسی، دانشکده علوم، دانشگاه فردوسی مشهد | ||
| چکیده | ||
| در سالهای اخیر، استفاده از متابولیتهای ثانویه مانند آنتی‌بیوتیک‌ها به منظور کنترل زیستی عوامل بیماریزای گیاهی مورد توجه قرار گرفته است. این پژوهش با هدف جداسازی، شناسایی و بررسی فعالیت ضدمیکروبی جدایه‌هایی از Streptomyces از دریای عمان علیه سه بیمارگر گیاهی Fusarium oxysporum، Xanthomonas campestris و Erwinia amylovora اجرا شد. سویه‌های Streptomyces روی محیط کشت‌های نشاسته کازیین اگار، گلیسرول گلیسین آگار و نشاسته نیترات آگار در غلظت‌های نمکی متفاوت جداسازی شدند. فعالیت ضدمیکروبی سویه‌ها با استفاده از کشت تقاطعی و اندازهگیری هاله عدم رشد در برابر بیمارگرهای گیاهی اندازهگیری شد. 90% این سویه‌ها قادر به تولید هاله مهارکنندگی رشد در برابر باکتری X. campestris بودند که بالاترین هاله 1±15 میلی‌متر به‌دست آمد. دو سویه 130AC و 275AC برای استخراج ترکیب ضدمیکروبی انتخاب شدند. این سویه‌ها در محیطهای پیشکشت و تخمیری مختلفی کشت داده شدند. استفاده از محیطهای تخمیری مختلف نشان میدهد که محیط دارای گلیسرول و سویا هاله بازدارندگی را افزایش میدهد. سویه‌های به‌دست آمده براساس ژن SrRNA16 متعلق به گونه‌هایStreptomyces sp. می‌باشند. جهت استخراج ترکیب ضدمیکروبی از حلال آلی دی‌کلرومتان، کلروفرم و اتیل‌استات استفاده گردید. حلال دی‌کلرومتان در سویه 130 ACو کلروفرم در سویه 275AC با ایجاد هاله مهارکنندگی 21 و 25 میلی‌متر مؤثرتر از سایر حلال‌ها بودند. این مطالعه نشان میدهد که برخی جدایه‌های Streptomyces در محیطهای دریایی کمتر کاوش شده، دارای قابلیت کنترل باکتریهای بیماریزای گیاهی می‌باشند که می‌توانند مورد استفاده قرار گیرند. | ||
| کلیدواژه‌ها | ||
| Streptomyces دریایی؛ بیمارگرهای گیاهی؛ دریای عمان؛ فعالیت ضدمیکروبی | ||
| عنوان مقاله [English] | ||
| Evaluation of antimicrobial activity some of marine Streptomyces sp. against three plant pathogens | ||
| نویسندگان [English] | ||
| Mahsa Shams1؛ Bahar Shahnavaz2 | ||
| 1Former M. Sc. Student, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Iran | ||
| 2Assistant Professor, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Iran | ||
| چکیده [English] | ||
| In recent years, application of secondary metabolites such as antibiotics has been considered as a biological control method for plant pathogens. This study aimed to isolate, identify and evaluate the antimicrobial activity of Streptomyces species in Oman Sea against three plant pathogens including Fusarium oxysporum, Xanthomonas campestris and Erwinia amylovora. The Streptomyces strains were isolated on different media including starch casein agar, glycerol glycine agar and starch nitrate agar with different NaCl concentration. Preliminary antimicrobial activity was investigated using cross streak method and evaluated by measuring the zone of growth inhibition against plant pathogens. Ninety percent of the strains were able to inhibit the growth of X. campestris strain and the highest inhibition zone was 15±1 mm. Two strains, AC275 and AC130 were selected to extract the antimicrobial compound. The isolates were cultured in different seed cultures and fermentation media. The application of different fermentation media shows that glycerol and soybeans cultures increase inhibition zone. Based on 16S rRNA gene analysis, two strains were belonged to Streptomyces sp. The organic solvents including dichloromethane, chloroform and ethyl acetate were used in order to extract the antimicrobial compound. Dichloromethane in AC130 and chloroform in AC275 were more effective than other solvents by creating an inhibition zone of 21 and 25 mm respectively. This study shows that the Streptomyces species in less explored marine environment have the ability to control plant pathogenic bacteria. | ||
| کلیدواژه‌ها [English] | ||
| Antimicrobial activity, Marin Streptomyces, plant pathogens, sea Oman | ||
| مراجع | ||
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