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القای مقاومت توسط بتا آمینوبوتریک اسید (BABA) علیه بیماری پوسیدگی فوزاریومی ساقه و ریشۀ خیار | ||
دانش گیاهپزشکی ایران | ||
مقاله 10، دوره 45، شماره 2، مهر 1393، صفحه 299-307 اصل مقاله (428.02 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijpps.2014.53506 | ||
نویسندگان | ||
حمیدرضا علیزاده* 1؛ خدیجه سالاری2 | ||
1استادیار، گروه گیاهپزشکی، دانشکدۀ کشاورزی، دانشگاه جیرفت | ||
2مربی، گروه گیاهپزشکی، دانشکدۀ کشاورزی، دانشگاه جیرفت | ||
چکیده | ||
القای مقاومت در گیاهان یکی از روشهای جدید در مدیریت بیماریهای گیاهی است. بتا آمینوبوتیریک اسید (BABA) از ترکیباتی است که قادر به القای مقاومت در گیاهان است. در این تحقیق القای مقاومت توسط این ترکیب علیه قارچ Fusarium oxysporum f. sp. radices-cucumerinum F42 در گیاه خیار بررسی شده است. این ترکیب قادر به کاهش معناداری در شدت بیماری از طریق القای مقاومت گردید. بررسی کمی بیان ژنهای pr1 و lox1 با روش QPCR بیانگر افزایش بیان ژن pr1 بود که دلالت بر القای مسیر مقاومت وابسته به سالیسیلیک اسید دارد. بررسی بیان ژنهای کیتیناز و بتا-1 و 3-گلوکاناز نشان داد که BABA باعث ایجاد حالت آمادهباش در بیان این ژنها میشود که پس از تلقیح بیمارگر بیان این ژنها افزایش معناداری نسبت به شاهد نشان داد. | ||
کلیدواژهها | ||
حالت آمادهباش؛ سالیسیلیک اسید؛ کیتیناز؛ گلوکاناز؛ مقاومت سیستمیک | ||
مراجع | ||
Alizadeh, H., Behboudi, K., Ahmadzadeh, M., Javan-Nikkhah, M., Zamioudis, C., Pieterse, C.M.J. & Bakker, P.A.H.M. (2013). Induced systemic resistance in cucumber and Arabidopsis thaliana by the combination of Trichoderma harzianum Tr6 and Pseudomonas sp. Ps14. Biological Control 65, 14-23.
Altamiranda, E.A.G., Andreu, A.B., Daleo, G.R. & Olivieri, F.P. (2008). Effect of β-aminobutyric acid (BABA) on protection against Phytophthora infestans throughout the potato crop cycle. Australasian Plant Pathology 37, 421-427.
Baysal, Ö., Gürsoy, Y.Z., Örnek, H. & Duru, A. (2005). Induction of oxidants in tomato leaves treated with DL-β-Amino butyric acid (BABA) and infected with Clavibacter michiganensis ssp. michiganensis. European Journal of Plant Pathology 112, 361-369.
Chamsai, J., Siegrist, J. & Buchenauer, H. (2004). Mode of action of the resistance-inducing 3-aminobutyric acid in tomato roots against Fusarium wilt. Journal Plant Disease Protection 111, 273-291.
Cohen, Y. (1994). 3-Aminobutyric acid induces systemic resistance against Peronospora tabacina. Physiological and Molecular Plant Pathology 4, 273-288.
Cohen, Y. (2001). The BABA story of induced resistance. Phytoparasitica 29, 375-378.
Cohen, Y., Niderman, T., Mosinger, E. & Fluhr, R. (1994). [beta]-Aminobutyric Acid Induces the Accumulation of Pathogenesis-Related Proteins in Tomato (Lycopersicon esculentum L.) Plants and Resistance to Late Blight Infection Caused by Phytophthora infestans. Plant Physiology 104, 59-66.
Cohen, Y., Rubin, A. & Kilfin, G. (2010). Mechanisms of induced resistance in lettuce against Bremia lactucae by DL-β-amino-butyric acid (BABA). European Journal of Plant Pathology 126, 553-573.
Conrath, U., Beckers, G.J., Flors, V., Garcia-Agustin, P., Jakab, G., Mauch, F., Newman, M.A., Pieterse, C.M.J., Poinssot, B., Pozo, M.J., Pugin, A., Schaffrath, U., Ton, J., Wendehenne, D., Zimmerli, L. & Mauch-Mani, B. (2006). Priming: getting ready for battle. Molecular Plant-Microbe Interactions 19, 1062-1071.
Doxey, A.C., Yaish, M.W., Moffatt, B.A., Griffith, M. & McConkey, B.J. (2007). Functional divergence in the Arabidopsis beta-1,3-glucanase gene family inferred by phylogenetic reconstruction of expression states. Molecular Biology and Evolution 24, 1045-1055.
Hamiduzzaman, M.M., Jakab, G., Barnavon, L., Neuhaus, J.M. & Mauch-Mani, B. (2005). beta-Aminobutyric acid-induced resistance against downy mildew in grapevine acts through the potentiation of callose formation and jasmonic acid signaling. Molecular Plant Microbe Interaction 18, 819-829.
Hwang, B.K., Sunwoo, J.Y., Kim, Y.J. & Kim, B.S. (1997). Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems to Phytophthora capsici. Physiological and Molecular Plant Pathology 51, 305-322.
Jakab, G., Cottier, V., Toquin, V., Rigoli, G., Zimmerli, L., Métraux, J.-P. & Mauch-Mani, B. (2001). β-Aminobutyric Acid-induced Resistance in Plants. European Journal of Plant Pathology 107, 29-37.
Jeun, Y.C., Park, K.S., Kim, C.H., Fowler, W.D. & Kloepper, J.W. (2004). Cytological observations of cucumber plants during induced resistance elicited by rhizobacteria. Biological Control 29, 34-42.
Justyna, P.-G. & Ewa, K. (2013). Induction of resistance against pathogens by β-aminobutyric acid. Acta Physiologiae Plantarum 35, 1735-1748.
Marcucci, E., Aleandri, M.P., Chilosi, G. & Magro, P. (2010). Induced Resistance by β-Aminobutyric Acid in Artichoke against White Mould Caused by Sclerotinia sclerotiorum. Journal of Phytopathology 158, 659-667.
Melan, M.A., Dong, X., Endara, M.E., Davis, K.R., Ausubel, F.M. & Peterman, T.K. (1993). An Arabidopsis thaliana Lipoxygenase Gene Can Be Induced by Pathogens, Abscisic Acid, and Methyl Jasmonate. Plant Physioliology 101, 441-450.
Migocka, M. & Papierniak, A. (2011). Identification of suitable reference genes for studying gene expression in cucumber plants subjected to abiotic stress and growth regulators. Molecular Breeding 28, 343-357.
Pape, S., Thurow, C. & Gatz, C. (2010). The Arabidopsis PR-1 promoter contains multiple integration sites for the coactivator NPR1 and the repressor SNI1. Plant Physioliology 154, 1805-1818.
Pfaffl, M.W. (2001). A new mathematical model for relative quantification in real-time RT–PCR. Nucleic Acids Research 29, e45.
Ramirez, V., Van der Ent, S., Garcia-Andrade, J., Coego, A., Pieterse C. M. & Vera, P. (2010). OCP3 is an important modulator of NPR1-mediated jasmonic acid-dependent induced defenses in Arabidopsis. BMC Plant Biology 10: 199.
Sahai, A.S. & Manocha, M.S. (1993). Chitinases of fungi and plants: their involvement in morphogenesis and host-parasite interaction. FEMS Microbiology Reviews 11, 317-338.
Seidl, V. (2008). Chitinases of filamentous fungi: a large group of diverse proteins with multiple physiological functions. Fungal Biology Reviews 22, 36-42.
Shoresh, M., Yedidia, I. & Chet, I. (2005). Involvement of Jasmonic Acid/Ethylene Signaling Pathway in the Systemic Resistance Induced in Cucumber by Trichoderma asperellum T203. Phytopathology 95, 76-84.
Siegrist, J., Orober, M. & Buchenauer, H. (2000). β-Aminobutyric acid-mediated enhancement of resistance in tobacco to tobacco mosaic virus depends on the accumulation of salicylic acid. Physiological and Molecular Plant Pathology 56, 95-106.
Ton, J., Jakab, G., Toquin, V., Flors, V., Iavicoli, A., Maeder, M.N., Metraux, J.P. & Mauch-Mani, B. (2005). Dissecting the beta-aminobutyric acid-induced priming phenomenon in Arabidopsis. Plant Cell 17, 987-999.
Ton, J. & Mauch-Mani, B. (2004). Beta-amino-butyric acid-induced resistance against necrotrophic pathogens is based on ABA-dependent priming for callose. Plant Journal 38, 119-130.
Van der Does, D. 2012. Hormonal crosstalk in plant immunity. Ph. D. dissertation. Utrecht University,the Netherlands.
Wan, H., Zhao, Z., Qian, C., Sui, Y., Malik, A.A. & Chen, J. (2010). Selection of appropriate reference genes for gene expression studies by quantitative real-time polymerase chain reaction in cucumber. Analytical Biochemistry 399, 257-261.
Ward, E.R., Uknes, S.J., Williams, S.C., Dincher, S.S., Wiederhold, D.L., Alexander, D.C., Ahl-Goy, P., Metraux, J.P. & Ryals, J.A. (1991). Coordinate Gene Activity in Response to Agents That Induce Systemic Acquired Resistance. The Plant Cell Online 3, 1085-1094.
Zimmerli, L., Jakab, G., Metraux, J.P. & Mauch-Mani, B. (2000). Potentiation of pathogen-specific defense mechanisms in Arabidopsis by beta -aminobutyric acid. Proceeding of the National Academy of Science USA 97, 12920-12925.
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