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بررسی ویژگی‌های زیستی باکتریPseudomonas fluorescens UTPF68 و ارزیابی توان بیوکنترلی آن علیه Phytophthora drechsleri عامل بوته‌میری خیار | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 3، دوره 3، شماره 2، مهر 1393، صفحه 105-116 اصل مقاله (497.41 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2014.53466 | ||
| نویسندگان | ||
| سمیه السادات قافله باشی1؛ فاطمه جمالی2؛ مسعود احمدزاده* 3 | ||
| 1دانش‌آموختة کارشناسی ارشد، گروه گیاه‌پزشکی، دانشکدة علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| 2استادیار، گروه زراعت و اصلاح نباتات، دانشکدة کشاورزی و منابع طبیعی، دانشگاه خلیج فارس بوشهر | ||
| 3استاد، گروه گیاه‌پزشکی، دانشکدة علوم و مهندسی کشاورزی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
| چکیده | ||
| در این تحقیق از باکتری Pseudomonas fluorescens UTPF68، عامل کنترل بیولوژیک، علیه Phytophthora drechsleri استفاده شد. ترکیبات فرّار سویة UTPF68 در سه محیط کشت NA، NAG و PDA به‌ترتیب 44/84 درصد، 55/83 درصد و 33/67 درصد بازدارندگی از رشد میسیلیومی P. drechsleri را در برداشت. نتایج نشان داد که متابولیت‌های خارج‌سلولی تولیدشده توسط سویة UTPF68 در محیط کشت PDA به‌طور کامل از رشد P. drechsleri ممانعت و در محیط کشت CMA به میزان 3/93 درصد از رشد بیمارگر جلوگیری کرد. سلول و عصارة سویة UTPF68 در سه غلظت 10 درصد، 25 درصد و 50 درصد، درون محیط مایع، به‌طور کامل از رشد P. drechsleriجلوگیری کرد. همچنین، این باکتری توانست هر سه آنتی‌بیوتیک DAPG، MAPG و Plt را در محیط کشت KBG تولید کند. نتایج نشان داد که این باکتری می‌تواند هر دو شرایط in vitro و in vivo را به کنترل P. drechsleri درآورد؛ به‌طوری که، کاربرد غلظت 108 سلول در هر میلی‌لیتر از این باکتری در خاک، خسارت ناشی از این بیمارگر را تا 60 درصد کاهش داد. مایه‌زنی این باکتری با خاک در حضور و نبود بیمارگر باعث بهبود فاکتورهای رشدی گیاه تحت شرایط گلخانه شد. به‌طوری که، از این لحاظ با شاهد سالم تفاوت معنی‌داری نشان داد. بررسی کلونیزاسیون ریشة خیار نشان داد که این باکتری بعد از گذشت دو هفته، توانست منطقة ریزوسفر با جمعیت 109×3/2 در گرم ریشه را کلونیزه کند. | ||
| کلیدواژه‌ها | ||
| آنتی‌بیوتیک؛ عصارة سلولی؛ کلونیزاسیون؛ کنترل بیولوژیک؛ متابولیت‌های خارج‌سلولی | ||
| عنوان مقاله [English] | ||
| Study biological properties of Pseudomonas fluorescens UTPF68, biocontrol agent against Phytophthora drechsleri | ||
| نویسندگان [English] | ||
| Somayeh Sadat Ghafelebashi1؛ Fatemeh Jamali2؛ Masoud Ahmadzadeh3 | ||
| 2Department of Agronomy and Plant Breeding, Faculty of Agriculture and Natural Resources, Perisan Gulf University, Bushehr | ||
| 3Professor, Department of Plant Protection, Faculty of Science and Agricultural engineering, University College of Agriculture and Natural resources, University of Tehran, Karaj | ||
| چکیده [English] | ||
| Pseudomonas fluorescens UTPF68, well-known biocontrol bacteria was used in this study. The volatile metabolites released from UTPF68 in NA, NAG, PDA media, could inhibit the growth of fungi P.drechsleri at the rate of 84.44, 83.55 and 67.33 percent, respectively. The results showed that antibiotics production in PDA and CMA media prevented the growth of P. drechsleri, 100% and 93%, respectively. The results showed that this strain is able to produce all three antibiotics DAPG, MAPG and the Plt. Also, the extracellular extracts of UTPF68 reduced the vegetative growth of P.drechsleri in extracts concentration. In addition, the bacterium effectiveness against the pathogen in greenhouse cucumber was studied. The bacterium under the in vitro and in vivo conditions was able to control the pathogen; So that, the application of bacterial concentration at 108 CFU/ml in to the soil, reduced the damage resulted from disease up to 60%. Also, the use of this bacterium, in the presence and absence of the pathogen as controls, increased the growth of cucumber. The bacterial colonization experiment showed that the population density of strain UTPF68 was increased on cucumber root system to 2.3×109 CFU. | ||
| کلیدواژه‌ها [English] | ||
| Antibiotic, Biocontrol, Colonization, extracellular extracts, the volatile metabolites | ||
| مراجع | ||
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