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jafari, javad, Ghasemzadeh-nava, Hamid, Akbarinejad, Vahid, Shahverdi, Abdolhossein. (1403). Effects of incubation temperature at 37°c on canine sperm quality before cooling to 5°c. , (), -. doi: 10.22059/ijvm.2024.380291.1005618
javad jafari; Hamid Ghasemzadeh-nava; Vahid Akbarinejad; Abdolhossein Shahverdi. "Effects of incubation temperature at 37°c on canine sperm quality before cooling to 5°c". , , , 1403, -. doi: 10.22059/ijvm.2024.380291.1005618
jafari, javad, Ghasemzadeh-nava, Hamid, Akbarinejad, Vahid, Shahverdi, Abdolhossein. (1403). 'Effects of incubation temperature at 37°c on canine sperm quality before cooling to 5°c', , (), pp. -. doi: 10.22059/ijvm.2024.380291.1005618
jafari, javad, Ghasemzadeh-nava, Hamid, Akbarinejad, Vahid, Shahverdi, Abdolhossein. Effects of incubation temperature at 37°c on canine sperm quality before cooling to 5°c. , 1403; (): -. doi: 10.22059/ijvm.2024.380291.1005618

Effects of incubation temperature at 37°c on canine sperm quality before cooling to 5°c

Iranian Journal of Veterinary Medicine
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 21 شهریور 1403
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22059/ijvm.2024.380291.1005618
نویسندگان
javad jafari1؛ Hamid Ghasemzadeh-nava* 2؛ Vahid Akbarinejad2؛ Abdolhossein Shahverdi3
1Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran
3Department of Embryology, Reproduction Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran.
چکیده
BACKGROUND: One of the most important aspects of artificial insemination in animals is the practical design of long-term preservation of sperm. Although there are many reports on canine sperm storage methods, but an improved system for chilling of canine spermatozoa is required for successful breeding programs in companion and working dog colonies.

OBJECTIVES: The aim of this study was to compare the effects of Bain-marie thermal shock on dog sperm parameters before cooling.

METHODS: Ejaclated sperm from testes of mature healthy dogs in veterinary center were collected and divided into two groups: C:TRIS+ egg yolk 20% (control, without incubation at 37°C), T: TRIS+ egg yolk 20% (treatment group, incubation at 37°C for 10 minutes). Evaluation of cooled Sperm was done by CASA for motility test, morphology, Eosin- Nigrosin vital staining and Hypo-Osmotic Swelling Test (HOST) after cooling.

RESULTS: In heat shock samples, total and progressive motility, VCL, VSL, VAP, LIN, STR, the proportion of sperm with normal morphology, the proportion of viable sperm and the proportion of sperm positive for HOST were not affected by the interaction of group by time. However, they were lower in treatment than control group (P < 0.05) and they decreased over the course of preservation (P < 0.001).

CONCLUSIONS: Keeping sperm at a temperature of 37°C and applying heat shock by Bain-Marie has extremely negative effects on the quality of ejaculated sperm.
کلیدواژه‌ها
Bain-marie؛ CASA؛ Dog؛ Incubation؛ Spermatozoa
آمار
تعداد مشاهده مقاله: 122





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