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Molecular and Clinical Study of Feline Infectious Peritonitis Virus in Iran Shows a Paraphyletic Tree; Emphasizes the "Internal Mutation" Hypothesis. | ||
| Iranian Journal of Veterinary Medicine | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 06 تیر 1403 اصل مقاله (606.33 K) | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2024.337532.1005247 | ||
| نویسندگان | ||
| Shahram Jamshidi1؛ Farnoosh Momeni1؛ Iraj Ashrafi Tamai2؛ Omid Madadgar* 2 | ||
| 1Department of Clinical Sciences,Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran. | ||
| 2Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
| چکیده | ||
| Objectives: This study aims to conduct a thorough investigation into the characteristics of Iranian FIPV, encompassing sequence analysis, and detailed examination of laboratory and clinical findings. The primary objective is to unravel the hypothesized genesis of the FIP virus, with a specific focus on the M gene level. Methods: Our methodology involved the examination of abdominal or thoracic fluids from 17 cats suspected of FIP, utilizing biochemical tests such as total serum protein, Albumin to Globulin (A/G) ratio, and the Rivalta test. Additionally, a molecular approach utilizing RT-PCR based on the Membrane (M) gene was employed. Sequence analysis of five crucial residues in the M genes and the subsequent construction of a phylogenetic tree using five sequenced viruses further enriched our investigation. Results: The study confirmed FIP in 6 out of 17 cats through the Rivalta test, guiding subsequent evaluations. Noteworthy gender disparities in FIP occurrences among young cats (9-30 months old) were observed, with males exhibiting a twofold higher incidence compared to females. Affected cats within the 9-30 months age range consistently exhibited an A/G ratio below 0.66 and total serum protein exceeding 0.43 g/dl. Cavity fluid cytology indicated non-degenerated macrophages and neutrophils against a basophilic background, due to a high protein percentage, confirming FIP diagnosis. Importantly, sequence analysis of five M protein amino acid hotspots revealed negligible differences in nucleotide sequences between FECoV and FIPV, aligning with their biotypic pattern. Conclusions: the phylogenetic tree generated in this study displayed a paraphilic pattern, emphasizing the "Internal Mutation" hypothesis, which suggests viral mutations occur within the cat's body and there are no significant differences in FECoV and FIPV-generating viruses. These findings contribute valuable insights to the discourse surrounding FIP pathogenesis, potentially guiding future diagnostic and therapeutic approaches. | ||
| کلیدواژه‌ها | ||
| FIPV؛ Biochemical Tests؛ Phylogenetic Analysis؛ Rivalta test؛ Iran | ||
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