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ویژگیهای بیولوژیکی و مولکولی چهار جدایه ویروس وای سیبزمینی (PVY) براساس بخشی از ناحیه ژنی رمزکننده پروتئین پوششی | ||
دانش گیاهپزشکی ایران | ||
دوره 54، شماره 2، اسفند 1402، صفحه 349-371 اصل مقاله (2.91 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijpps.2024.371771.1007053 | ||
نویسندگان | ||
پرستو پورشریفی1؛ اکبر دیزجی* 2؛ پریسا شریفی نظام آباد3؛ مینا کوهی حبیبی4؛ هادی خاطری5 | ||
1گروه گیاهپزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران | ||
2گروه گیاهپزشکی، دانشکده کشاورزی، دانشکدگان کشاورزی و منابع طبیعی، دانشگاه تهران، کرج، ایران. | ||
3بخش تحقیقات گیاهپزشکی، مرکز تحقیقات و آموزش کشاورزی و منابع طبیعی استان گلستان، گرگان، ایران. | ||
4گروه گیاهپزشکی دانشکده کشاورزی دانشگاه تهران. کرج. ایران | ||
5عضو هیأت علمی گروه گیاهپزشکی، دانشکده کشاورزی، دانشگاه رازی، کرمانشاه، ایران | ||
چکیده | ||
ویروس وای سیبزمینی (Potato virus Y, PVY) یکی از ویروسهای محدودکننده تولید توتون و سیبزمینی در دنیا میباشد. به منظور بررسی خصوصیات بیولوژیکی و مولکولی جدایههای میزبانی ویروس وای سیبزمینی، طی سالهای 1389 و 1390، در مجموع 1178 نمونه از گیاهان توتون، سیبزمینی، فلفل و علف هرز عروسک پشت پرده که دارای علائم موزائیک بودند از هشت شهرستان مختلف کشور شامل کرج، ارومیه، رشت، سنندج، شیراز، قزوین، ورامین و همدان جمعآوری شد. آلودگی نمونهها به PVY با آزمون سرولوژیکی ساندویچ دو طرفه الیزا (double antibody sandwich enzyme-linked immunosorbent assay, DAS-ELISA) بررسی و میزان آلودگی در بین نمونههای توتون، سیبزمینی، عروسکپشتپرده و فلفل به ترتیب 2/27، 4/20، 75/18 و صفر درصد برآورد گردید. دامنه میزبانی آزمایشی چهار جدایه منتخب ویروس وای سیبزمینی (Po2، Po5، Tob2 و Ph1) روی 12 گونه گیاه محک در شرایط گلخانه تفاوتهای جزئی داشت. پس از تکثیر انتهای 3' ژنوم جدایهها به اندازه 1200 جفت باز، درخت تبارزائی براساس توالی نوکلئوتیدی یک سوم بالادست ناحیه ژنی رمزکننده پروتئین پوششی بازسازی شد که جدایههای Tob2، Ph1 و Po5 در کلاد PVYN/NTN و جدایه Po2 در کلاد PVYO قرار گرفتند. نتایج IC-RT-PCR با استفاده از آغازگرهای اختصاصی سویه، و مقایسه توالیهای نوکلئوتیدی و بررسی موتیفهای اختصاصی سویه در همردیفهای توالی آمینواسیدی پیش بینی شده انتهای آمینی پروتئین پوششی، نتایج واکاوی تبارزائی را تایید کردند. این اولین گزارش از آلودگی گونه Physalis divaricata به سویه PVYN/NTN در دنیا میباشد. | ||
کلیدواژهها | ||
تبارزائی؛ سویه؛ سرولوژی؛ عروسک پشت پرده | ||
مراجع | ||
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