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کاوش ژنومی نشانه های انتخاب جهت شناسایی مکان های کروموزومی مرتبط با بیماری یون در گاوهای هلشتاین | ||
علوم دامی ایران | ||
دوره 55، شماره 3، مهر 1403، صفحه 437-450 اصل مقاله (1.01 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijas.2024.367917.653972 | ||
نویسندگان | ||
فاطمه ناوشکی1؛ حسین مرادی شهربابک* 2؛ علی صادقی سفید مزگی3؛ جوهان سولکنر4؛ مهدی جوان نیکخواه5 | ||
1گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی دانشگاه تهران، کرج، البرز، ایران: | ||
2گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی دانشگاه تهران، کرج، البرز، ایران | ||
3گروه علوم دامی ، دانشکده کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران. | ||
4گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی دانشگاه بوکو، وینا، اتریش | ||
5گروه علوم دامی، پردیس ارس، دانشگاه تهران، جلفا، ایران | ||
چکیده | ||
انتخاب باعث ایجاد نشانههای انتخاب در سطح ژنوم میشود. شناسایی نشانههای انتخاب در حیوانات در جهت ارتقا صفات اقتصادی و کاهش بیماریها، یکی از اصلیترین و چالش برانگیزترین تحقیقات در زمینه ژنتیک جمعیت است. در این تحقیق، با هدف شناسایی مناطق ژنومی تحت انتخاب مثبت بین جمعیتهای گاوهای مبتلا به بیماری یون و سالم هلشتاین، پویش گستره ژنوم با استفاده از چندشکلیهای تک نوکلئوتیدی (SNP) انجام شد. این تحقیق بر روی گاوهای گاوداری فوکا در اصفهان بر روی 145 راس گاو هلشتاین انجام شد. بر اساس تراشههای 30K شرکت ایلومینا تعیین ژنوتیپ و گاوها به دو گروه بیمار و سالم گروه بندی شدند. گروه بیمار شامل 45 راس و گروه سالم شامل 100 راس گاو بود. در این مطالعه برای شناسایی مناطق ژنومی تحت انتخاب از دو آماره FST و XP-EHH استفاده شد. ژنهای شناسایی شده توسط آماره FST در دو جمعیت بیمار و سالم شاملRAB37, ZC3H10, ESR1 HSD17B6, KCNC4, ERBB3 و NACA بودند. ژنهای شناسایی شده توسط آماره XP-EHH در دو جمعیت بیمار و سالم شاملAK1, ATP8A1, BTBD1, C1GALT1, CCDC6, CEP295, CLGN, CLSTN2, EHHADH, ERBB4, FRK, GRID2, GRIP1 و LRP6 بودند. اکثر ژن های شناسایی شده در این مطالعه با ایمنی، بیماری هایی مثل سرطان، شیردهی، ماهیچه های اسکلتی، چرخه فحلی، مصرف خوراک، چسبندگی اسپرم و رشد در ارتباط بود، که جزو صفات و ویژگی های مهم زیستی جاندار قرار می گیرد. مطمئناً مطالعات گستردهتر با تعداد نمونههای بیشتر، درک بهتری از ژنهای کاندید برای بیماری یون در گاو و طراحی برنامههای اصلاح نژادی موفق در جهت کاهش هزینههای ناشی از بیماری، ایجاد خواهد نمود. | ||
کلیدواژهها | ||
بیماری یون؛ گاو هلشتاین؛ نشانه-های انتخاب؛ FST؛ XP-EHH | ||
مراجع | ||
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