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پویش ژنوم برخی صفات تولیدمثلی گاو هلشتاین ایران | ||
علوم دامی ایران | ||
دوره 55، شماره 3، مهر 1403، صفحه 565-581 اصل مقاله (1.45 M) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijas.2023.359774.653947 | ||
نویسندگان | ||
مصطفی صادقی* ؛ نرگس مداحی؛ اردشیر نجاتی جوارمی؛ سید رضا میرائی آشتیانی؛ علی جلیل سرقلعه | ||
گروه علوم دامی، دانشکده کشاورزی و منابع طبیعی، دانشگاه تهران، دانشگاه تهران، تهران، ایران | ||
چکیده | ||
هدف از انجام این تحقیق شناسایی نشانگرهای تک نوکلئوتیدی موثر بر برخی صفات تولیدمثلی در گاوهای شیری هلشتاین ابران است. برای این منظور از نمونه مو 150 راس گاو که بین سالهای 1390-1392 در یکی از گاوداری های شرکت فردوس پارس متولد شده بودند، جهت تعیین ژنوتیپ با تراشه حاوی SNP 30108 استفاده شد. بعد از انجام مراحل کنترل کیفیت دادهها با استفاده از روش تجزیه واریانس حداقل مربعات با استفاده از رویه GLM، آنالیز GWAS انجام گردید. نتایج آنالیز نشان داد 2 تغییر تک نوکلئوتیدی با صفت فاصله زایش تا آبستنی و 12 تغییر تک نوکلئوتیدی با صفت فاصله بین اولین و آخرین تلقیح ارتباط معنیداری (pValue<0.04) داشتند، در صفت روزهای باز 11 و برای صفت تعداد تلقیح به ازای آبستنی نیز 5 تغییر تک نوکلئوتیدی معنیدار (pValue<0.04) وجود داشت. بررسی ها بعد از مکان یابی ژنها تعدادیQTL و ژنهای مختلفی را شناسایی کرد که بر صفات مورد بررسی اثرگذار یا کنترل کننده بودند. از جمله این ژنها میتوان به ژن NUF2 در کروموزوم 3 که بر تکامل سلول تخم، ژن ANAPC1 در کروموزوم 11 که بر جنبانی اسپرم، ژن PITX2 در کروموزوم 6 که بر تولید شیر، ژن ELOVL6 در کروموزوم 6 که بر افزایش وزن بدن، موثر است اشاره نمود. به طور کلی میتوان نتیجه گرفت که کروموزوم 3، 6 و 11 دارای تغییرات تک نوکلئوتیدی هستند که در ارتباط قوی تری با صفات تولیدمثلی بوده و در حاشیه همین تغییرات تک نوکلئوتیدی، QTL و ژن هایی یافت شد که مرتبط با صفات تولید مثلی بوده اند. | ||
کلیدواژهها | ||
روزهای باز؛ فاصله زایش تا اولین تلقیح؛ فاصله اولین و آخرین تلقیح | ||
مراجع | ||
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