Ghahremani, Mehran, Pourrezaei, Elham, Naghibi, Saeed, Jafari, Afshar. (1401). The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium. , 15(1), 45-52. doi: 10.22059/sshr.2023.361500.1106
Mehran Ghahremani; Elham Pourrezaei; Saeed Naghibi; Afshar Jafari. "The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium". , 15, 1, 1401, 45-52. doi: 10.22059/sshr.2023.361500.1106
Ghahremani, Mehran, Pourrezaei, Elham, Naghibi, Saeed, Jafari, Afshar. (1401). 'The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium', , 15(1), pp. 45-52. doi: 10.22059/sshr.2023.361500.1106
Ghahremani, Mehran, Pourrezaei, Elham, Naghibi, Saeed, Jafari, Afshar. The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium. , 1401; 15(1): 45-52. doi: 10.22059/sshr.2023.361500.1106

The effect of different exercise training protocols on MMP-9 and TIMP-1 gene expression in rat myocardium

Sport Sciences and Health Research
دوره 15، شماره 1، فروردین 2023، صفحه 45-52    اصل مقاله (435.89 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22059/sshr.2023.361500.1106
نویسندگان
Mehran Ghahremani1؛ Elham Pourrezaei1؛ Saeed Naghibi2؛ Afshar Jafari* 3
1Department of Physical Education and Sport Sciences, Physical Education and Sport Sciences, Payame Noor University (Alborz), Karaj, Iran.
2Department of Physical Education and Sport Sciences, Physical Education and Sport Sciences, Payame Noor University, Tehran, Iran.
3Department of Biological Sciences in Sport, Faculty of Sport Sciences and Health, Shahid Beheshti University, Tehran, Iran.
چکیده
Background: There are conflicting findings regarding the effect of various training modalities on the cardiovascular disease mechanisms induced by undesirable modifications in extracellular matrix (ECM) factors such as MMP-9 and TIMP-1.
Aim: This study was conducted to investigate the effect of different training protocols on the MMP-9 and TIMP-1 gene expression in the myocardium of Wistar male rats.
Materials and Methods: Forty-eight adult Wistar male rats (aged: 8 weeks) were randomly allocated into two control (Con8w/base, n=8; and Con16w/end control, n=8) and four training groups (MICT, n=8; PICT, n=8; HIIT, n=8; and MIST, n=8). The training protocols consisted of 8 weeks (5 days/week) of Moderate-Intensity, Continuous Training (MICT: 37 min running with 65% vVO2max), Moderate-Intensity, Swimming Training (MIST: 30 min free-swimming), Progressive-Intensity, Continuous Training (PICT: 28 min running with a progressive increase in the incline of the treadmill by 2% per week), and High-Intensity Interval Training (HIIT: four running bouts 4 min with 90 to 100% vVO2max + 3 min with 50-65% vVO2max). Myocardial samples were isolated 24 hours after the last training session. Gene expression was determined using the real-time PCR method. All data (M±SD) were analyzed by one-way ANOVA and Tukey's post-hoc tests at α ≤0.05.
Results: There were significant differences between MMP-9 and TIMP-1 expression in the animals myocardium after different exercise training protocols (P<0.01). The lowest and highest MMP-9/TIMP-1 ratios were in the HIIT and MICT protocols, respectively.
Conclusion: According to our findings, the HIIT protocol may play a more influential role in enhancing myocardial ECM-remodeling and fibrosis indicators during lifespan than the other exercise protocols.
کلیدواژه‌ها
angiogenesis؛ exercise modalities؛ heart fibrosis؛ gene expression؛ matrix metalloproteinase
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