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جداسازی، غربالگری و شناسایی ریزوباکتری‌های محرک رشد مقاوم به تنش‌های غیرزیستی از میکروبیوم گیاه یونجه (Medicago sativa) در خاک‌های خشک و شور استان کرمان | ||
| تحقیقات آب و خاک ایران | ||
| دوره 54، شماره 4، تیر 1402، صفحه 675-693 اصل مقاله (1.71 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijswr.2023.356810.669471 | ||
| نویسندگان | ||
| محبوبه ابوالحسنی زراعتکار* 1؛ احمد تاج آبادی پور2 | ||
| 1گروه علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه شهید باهنر کرمان، کرمان، ایران. | ||
| 2علوم و مهندسی خاک، دانشکده کشاورزی، دانشگاه ولی عصر (عج) رفسنجان، کرمان، ایران. | ||
| چکیده | ||
| این مطالعه با هدف بررسی خصوصیات ریزوباکتری‌های بومی محرک رشد از قبیل تثبیت بیولوژیکی نیتروژن، انحلال فسفات، تولید سیانید هیدروژن و اگزوپلی ساکارید در راستای تهیه کود زیستی مناسب و کارآمد منطقه انجام شد. در این مطالعه، 135 باکتری از ریشه گیاه یونجه در خاک‌های شور و خشک استان کرمان جداسازی، غربالگری و شناسایی شد و برخی از ویژگی‌های محرک رشد بودن این ریزوباکتری‌ها هم مورد بررسی قرار گرفت. بدین منظور ریزوباکتری‌ها از نظر توانایی تثبیت نیتروژن، انحلال فسفات‌های آلی و معدنی نامحلول در محیط کشت جامد و مایع، تولید سیانید هیدروژن و اگزوپلی ساکارید مورد بررسی قرار گرفتند. رشد ریزوباکتری‌ها در غلظت‌های مختلف کلرید سدیم (0، 1، 3 و 5 درصد) و غلظت‌های مختلف پلی اتیلن گلیکول 6000 (پتانسیل آبی 0، 1-، 2- و 5/3- مگاپاسکال) نیز مورد بررسی قرار گرفت. این بررسی‌ها نشان داد که 72 درصد ریزوباکتری‌ها در سطح 5 درصد نمک کلرید سدیم و 76 درصد در سطح پتانسیل آبی 5/3- مگاپاسکال تقریبا هیچ رشدی نداشتند. از طرفی 50 درصد ریزوباکتری‌ها توانایی تولید سیانید هیدروژن در شرایط شور را داشتند. سه باکتری SM16، SM65 و SM89 از کارآمدترین ریزوباکتری‌های تولیدکننده اگزوپلی ساکارید و دارای قدرت حل کنندگی فسفات بالا به ترتیب به میزان 102، 5/98 و 7/121 میلی‌گرم بر لیتر بودند و از طرفی جزء ریزوباکتری‌های مقاوم به تنش‌های غیرزیستی نیز قرار داشتند. بنابراین این سه سویه باکتریایی ایمن و کارآمد ممکن است پتانسیل خوبی برای تبدیل شدن به یک کود زیستی با کیفیت بالا جهت بهبود رشد محصولات زراعی در منطقه را داشته باشند. | ||
| کلیدواژه‌ها | ||
| اگزوپلی ساکارید؛ انحلال فسفات؛ تنش غیرزیستی؛ سیانید هیدروژن؛ کود زیستی | ||
| عنوان مقاله [English] | ||
| Isolation, screening and identification of growth-promoting rhizobacteria resistant to abiotic stresses from the microbiome of Alfalfa (Medicago sativa) in saline and arid soils in Kerman province | ||
| نویسندگان [English] | ||
| Mahboobeh Abolhasani Zeraatkar1؛ Ahmad Tajabadi Pour2 | ||
| 1Department of Soil Science, Agriculture Faculty, Shahid Bahonar University of Kerman, Kerman, Iran. | ||
| 2Department of Soil Science, Faculty of Agriculture, Vali-e-asr University of Rafsanjan, Kerman, Iran. | ||
| چکیده [English] | ||
| The purpose of this study is to investigate the characteristics of plant growth promoting rhizobacteria that stimulate plant growth, such as biological nitrogen fixation, phosphate solubilization, and produce hydrogen cyanide and exopolysaccharides, to prepare suitable and efficient biofertilizers. In this research, 135 Rhizobacteria strains were isolated, screened and identified from saline and arid soils in Kerman Province, and some of their growth-promoting characteristics were studied. The Rhizobacteria strains were studied with respect to their ability to fix nitrogen, solubilize insoluble organic and inorganic phosphates in solid and liquid culture media, and produce hydrogen cyanide and exopolysaccharides. Their growth at various sodium chloride concentrations (0, 1, 3, and 5%) and at different polyethylene glycol 6000 concentrations (water potential 0, -1, -2, and -3.5 MPa) was also investigated. According to the results, 72% and 76% of the Rhizobacteria strains exhibited almost no growth at 5% sodium chloride concentration and at water potential -3.5 MPa, respectively. In addition, 50% of them were able to produce hydrogen cyanide under saline conditions. Three of the strains (SM16, SM65, and SM89) were the most efficient producers of exopolysaccharides and had high phosphate solubilization ability (102, 98.5 and 121.7 mL/L, respectively) and were also among Rhizobacteria strains resistant to abiotic stresses. Three strain safe and efficient strains can have great potential to be used as a high quality biofertilizer for improving growth of crop plants in the region. | ||
| کلیدواژه‌ها [English] | ||
| Abiotic stress, Biofertilizer, Exopolysaccharide, Hydrogen cyanide, Phosphate solubilization | ||
| مراجع | ||
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آمار تعداد مشاهده مقاله: 200 تعداد دریافت فایل اصل مقاله: 140 |
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