Del Castillo De Loayza, Tatiana, Maldonado, Ingrid, Zirena Vilca, Franz. (1401). Identification and Quantification of Antibiotic Residues and Evaluation of Microbial Resistance to Antibiotics in Huatanay River Waters in Peru. , 9(3), 1236-1250. doi: 10.22059/poll.2023.352228.1718
Tatiana Del Castillo De Loayza; Ingrid Maldonado; Franz Zirena Vilca. "Identification and Quantification of Antibiotic Residues and Evaluation of Microbial Resistance to Antibiotics in Huatanay River Waters in Peru". , 9, 3, 1401, 1236-1250. doi: 10.22059/poll.2023.352228.1718
Del Castillo De Loayza, Tatiana, Maldonado, Ingrid, Zirena Vilca, Franz. (1401). 'Identification and Quantification of Antibiotic Residues and Evaluation of Microbial Resistance to Antibiotics in Huatanay River Waters in Peru', , 9(3), pp. 1236-1250. doi: 10.22059/poll.2023.352228.1718
Del Castillo De Loayza, Tatiana, Maldonado, Ingrid, Zirena Vilca, Franz. Identification and Quantification of Antibiotic Residues and Evaluation of Microbial Resistance to Antibiotics in Huatanay River Waters in Peru. , 1401; 9(3): 1236-1250. doi: 10.22059/poll.2023.352228.1718

Identification and Quantification of Antibiotic Residues and Evaluation of Microbial Resistance to Antibiotics in Huatanay River Waters in Peru

Pollution
دوره 9، شماره 3، پاییز 2023، صفحه 1236-1250    اصل مقاله (706.88 K)
نوع مقاله: Original Research Paper
شناسه دیجیتال (DOI): 10.22059/poll.2023.352228.1718
نویسندگان
Tatiana Del Castillo De Loayza1؛ Ingrid Maldonado2؛ Franz Zirena Vilca* 3
1Departamento Académico de Farmacia, Facultad de Ciencias de la Salud. UNSAAC, Cusco, Perú
2Programa de Doctorado en Ciencia, Tecnología y Medio Ambiente, Escuela de Posgrado, Universidad Nacional del Altiplano de Puno, Av. Floral N° 1153, Puno, Perú
3Laboratorio de Contaminantes Orgánicos y Ambiente del IINDEP de la Universidad Nacional de Moquegua, Perú, Urb Ciudad Jardín-Pacocha-Ilo, Perú
چکیده
The Huatanay River in Cusco-Peru, is affected by wastewater discharges along its course. In order to evaluate this impact, we evaluate antibiotic residues and their impact on the increase of bacterial resistance in the city of Cusco treatment plant. For this purpose, water samples from the influent and effluent of the treatment plant were analyzed by chromatographic methods; additionally, sensitivity tests were performed with three bacterial strains (Escherichia coli, Salmonella sp., and Klebsiella sp.), which were isolated from the same place. Six antibiotic residues were identified (ceftriaxone, amoxicillin, trimethoprim, sulfamethoxazole, dicloxacillin, and lincomycin). Those found in the highest concentration were: amoxicillin (91495 and 0 µg/L) and lincomycin (33970 and 10800 µg/L) in the influent and effluent, respectively. There is more resistance in the effluent than the influent in the case of E. coli shows resistance in the effluent to cephalexin (30 µg) and azithromycin (15 µg). Salmonella sp. is resistant to amoxicillin (15 µg), dicloxacillin (1 µg), lincomycin (2 µg), ceftriaxone (30 µg), cephalexin (30 µg), and ciprofloxacin (5 µg). Finally, Klebsiella sp. is sensitive to ceftriaxone (30 µg), amoxicillin (15 µg), and cephalexin (30 µg). This confirms that the antibiotic residues contained in the wastewater of Cusco generate resistance in the isolated bacteria.
کلیدواژه‌ها
Bacteria resistance؛ Chromatography؛ Emerging pollutants؛ Environmental Health؛ Pollution؛ Solid phase xtraction؛ wastewater
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