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تعیین میزان تغییرات اجزای اصلی زهر زنبورعسل در سه فصل و چند منطقه جغرافیایی ایران | ||
| علوم دامی ایران | ||
| دوره 54، شماره 1، فروردین 1402، صفحه 47-59 اصل مقاله (564.05 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijas.2022.339985.653877 | ||
| نویسندگان | ||
| مهدیه مبارک1؛ فاطمه غازیانی* 1؛ غلامعلی نهضتی پاقلعه1؛ محسن فرهادپور2 | ||
| 1گروه علوم دامی، دانشکدگان کشاورزی و منابع طبیعی دانشگاه تهران، کرج، ایران | ||
| 2گروه بیوتکنولوژی کشاورزی، مؤسسه ملی مهندسی ژنتیک و بیوتکنولوژی (NIGEB)، تهران، ایران | ||
| چکیده | ||
| زنبورعسل حشرهای کوچک با محصولات پرفایدهی بسیاری است. زهر یکی از این محصولات بوده که فواید درمانی و ارزش تجاری بالایی دارد. عوامل بسیاری بر زهر زنبور و اجزاء اصلی آن یعنی ملیتین، آپامین و فسفولیپاز A2 تاثیر گذارند. در این پژوهش اثر فصل و موقعیت جغرافیایی بر اجزاء اصلی زهر، طی سه فصل بهار، تابستان و پاییز در 8 شهر، در قالب طرح کاملا تصادفی با 4 تکرار (کندو) بررسی شده است. آنالیز نمونههای زهر مناطق مختلف با دستگاه HPLC نشان داد که اجزاء نامبرده زهر به غیر از آپامین (339/0= میزان همبستگی(، همبستگی با فصل نداشتند. میانگین پروتئین، ملیتین و فسفولیپاز A2 نمونهها، در طول 3 فصل تفاوت معنیداری نداشت(05/0<p). حداکثر میانگین درصد ملیتین (15/63 %) و فسفولیپاز A2 (19/8%) در بهار، و آپامین (03/4%) و پروتئین (89/48 میکروگرم بر 100 میلیگرم) در تابستان بود. بیشترین میزان پروتئین و آپامین زهر مربوط به شهر کرج (به ترتیب 28/50% و 33/4%)، بیشترین میزان ملیتین شهر خرم آباد (82/66%) و بیشترین میزان فسفولیپاز A2مربوط به شهر بیرجند (85/8%) بود. تاثیراتی که فصل از طریق دما بر روی ترکیبات زهر میگذارد میتواند علاوه بر تاثیر مستقیم بر زنبورعسل و غدد تولید زهر، از طریق تاثیر بر پوشش گیاهی منطقه و یا وجود تغییرات فصلی در ترکیبات زهر در کنار تاثیر موقعیت جغرافیایی بازتابی از تفاوت پوشش گیاهی باشد. | ||
| کلیدواژه‌ها | ||
| آپامین؛ زهر زنبورعسل؛ فصل؛ فسفولیپاز A2؛ ملیتین؛ منطقه جغرافیایی. | ||
| عنوان مقاله [English] | ||
| Determining the amount of changes in the main components of honey bee venom in three seasons and several geographical areas of Iran | ||
| نویسندگان [English] | ||
| Mahdieh Mobarak1؛ Fatemeh Ghaziani1؛ Gholam Ali Nehzati paghale1؛ Mohsen Farhadpour2 | ||
| 1Department of Animal Science Faculty of Agricultural Engineering, Karaj, Iran | ||
| 2Department of Agricultural Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran | ||
| چکیده [English] | ||
| Honey bees are small insects with many useful products. Venom is one of these products with therapeutic benefits and high commercial value. Many factors affect the bee venom and its main components, namely Melittin, Apamine and Phospholipase A2. This study investigates the effect of seasonal and geographical location on the venom composition in 8 cities, during three seasons of spring, summer and autumn, in a completely randomized design with 4 replications(hives). Analysis of venom samples from the target areas in different seasons by HPLC showed that the mentioned components, other than apamine (correlation=0.339), had no correlation with temperature. The mean amount of Protein, Melittin and Phospholipase A2 of the treatments did not differ significantly during the 3 seasons (p>0.05). The mean percentage of melittin (63.15 %) and phospholipase A2 (8.19 %) in spring; apamine (4.03 %) and protein (48.89 µg/100mg) were maximum in summer. The highest amount of Protein and Apamin in the venom was related to karaj (50.28% and 4.33% respectively), the highest amount of Melittin in khoramabad city and the highest Phospholipase A2 was in birjand city. The effects of season have on venom components through temperature can be in addition to the direct effect on honey bees and venom production glands, through the effect on vegetation of area or the presence of seasonal changes in the venom components in addition to the effect of geographical location, a reflection of the difference in vegetation. | ||
| کلیدواژه‌ها [English] | ||
| Apamine, Geographic area, Honeybee venom, Melittin, Phospholipase A2, Season | ||
| مراجع | ||
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