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Pourkhaloee, Ali, Khosh-Khui, Morteza, Barba-Gonzalez, Rodrigo. (1402). Somatic Embryogenesis of Tuberose (Agave amica L.) was Improved by Milk as a Potential Biostimulant in Plant Tissue Culture. , 10(3), 257-268. doi: 10.22059/ijhst.2022.330660.504
Ali Pourkhaloee; Morteza Khosh-Khui; Rodrigo Barba-Gonzalez. "Somatic Embryogenesis of Tuberose (Agave amica L.) was Improved by Milk as a Potential Biostimulant in Plant Tissue Culture". , 10, 3, 1402, 257-268. doi: 10.22059/ijhst.2022.330660.504
Pourkhaloee, Ali, Khosh-Khui, Morteza, Barba-Gonzalez, Rodrigo. (1402). 'Somatic Embryogenesis of Tuberose (Agave amica L.) was Improved by Milk as a Potential Biostimulant in Plant Tissue Culture', , 10(3), pp. 257-268. doi: 10.22059/ijhst.2022.330660.504
Pourkhaloee, Ali, Khosh-Khui, Morteza, Barba-Gonzalez, Rodrigo. Somatic Embryogenesis of Tuberose (Agave amica L.) was Improved by Milk as a Potential Biostimulant in Plant Tissue Culture. , 1402; 10(3): 257-268. doi: 10.22059/ijhst.2022.330660.504

Somatic Embryogenesis of Tuberose (Agave amica L.) was Improved by Milk as a Potential Biostimulant in Plant Tissue Culture

International Journal of Horticultural Science and Technology
دوره 10، شماره 3، مهر 2023، صفحه 257-268    اصل مقاله (1.21 M)
نوع مقاله: Research paper
شناسه دیجیتال (DOI): 10.22059/ijhst.2022.330660.504
نویسندگان
Ali Pourkhaloee* 1؛ Morteza Khosh-Khui2؛ Rodrigo Barba-Gonzalez3، 4
1Department of Horticultural Science, College of Agriculture, Vali-e-Asr University of Rafsanjan, Iran.
2Department of Horticulture, Faculty of Agriculture, Shiraz University, Shiraz, IR IRAN
3Unidad de Biotecnología Vegetal, Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Zapopan, Jalisco, 45019, Mexico
4CIATEJ · Plant Biotechnology Unit
چکیده
In this study, an efficient in vitro method was established for indirect somatic embryogenesis of tuberose from pedicel-derived calluses on MS medium. The effects of 2,4-D, NAA, BAP, and ABA on callus induction, callus growth, embryogenic callus formation, embryo maturation, and plantlet regeneration were evaluated. Combination of 0.5 mg L-1 2,4-D + 0.5 mg L-1 NAA resulted in the highest percentage of callus induction (100%) and callus quality. The highest average of callus growth was achieved at 1 mg L-1 2,4-D + 1 mg L 1 BAP. Embryogenic calluses were induced on the media containing 1 mg L-1 2,4-D after 90 days of subculturing. The highest number of matured somatic embryos per petri dish (81) and callus greening were observed on the medium containing 1 mg L-1 ABA + 45 g L-1 maltose. Further germination of embryos was observed on MS medium containing 10 to 15 ml L-1 goat colostrum and embryos with leaves regenerated after 90 days. Beside induction of osmotic pressure, colostrum is a rich source of organic nitrogen and calcium which play key roles in somatic embryogenesis. The whole plantlets were achieved after 60 days of subculturing on the media containing IBA (0.75 mg L-1) with a survival rate of 88%. In conclusion, the present study provides a suitable somatic embryogenesis system which may serve in micropropagation, genetic transformation, and ploidy manipulation of tuberose.
کلیدواژه‌ها
Bulbous plant؛ Indirect somatic embryo؛ Micropropagation؛ Organic additive؛ Plant tissue culture
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