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samiei, saber, tajadod, golnaz, Zarghami, Reza, mirzai, masoumeh. (1401). A 2,4-D-Free Combined Direct Organogenesis-Indirect Somatic Embryogenesis Protocol for Mass Propagation of Date Palm (Phoenix dactylifera L.) cv.Barhi. , 33(2), 125-132. doi: 10.22059/jsciences.2022.333363.1007684
saber samiei; golnaz tajadod; Reza Zarghami; masoumeh mirzai. "A 2,4-D-Free Combined Direct Organogenesis-Indirect Somatic Embryogenesis Protocol for Mass Propagation of Date Palm (Phoenix dactylifera L.) cv.Barhi". , 33, 2, 1401, 125-132. doi: 10.22059/jsciences.2022.333363.1007684
samiei, saber, tajadod, golnaz, Zarghami, Reza, mirzai, masoumeh. (1401). 'A 2,4-D-Free Combined Direct Organogenesis-Indirect Somatic Embryogenesis Protocol for Mass Propagation of Date Palm (Phoenix dactylifera L.) cv.Barhi', , 33(2), pp. 125-132. doi: 10.22059/jsciences.2022.333363.1007684
samiei, saber, tajadod, golnaz, Zarghami, Reza, mirzai, masoumeh. A 2,4-D-Free Combined Direct Organogenesis-Indirect Somatic Embryogenesis Protocol for Mass Propagation of Date Palm (Phoenix dactylifera L.) cv.Barhi. , 1401; 33(2): 125-132. doi: 10.22059/jsciences.2022.333363.1007684

A 2,4-D-Free Combined Direct Organogenesis-Indirect Somatic Embryogenesis Protocol for Mass Propagation of Date Palm (Phoenix dactylifera L.) cv.Barhi

Journal of Sciences, Islamic Republic of Iran
دوره 33، شماره 2، مهر 2022، صفحه 125-132    اصل مقاله (743.38 K)
نوع مقاله: Original Paper
شناسه دیجیتال (DOI): 10.22059/jsciences.2022.333363.1007684
نویسندگان
saber samiei1؛ golnaz tajadod1؛ Reza Zarghami* 2؛ masoumeh mirzai1
11 Department of Biological Sciences, Islamic Azad University, Tehran-North Branch, Tehran, Islamic Republic of Iran
22 Department of Tissue and Cell Culture, Agricultural Biotechnology Research Institute of Iran (ABRII), Agricultural Research, Education and Extension Organization (AREEO), Karaj 3135933151, Islamic Republic of Iran
چکیده
A 2,4-D-free protocol for indirect asexual embryogenesis from root explants of date palm cv. Barhi has been established. Different mixtures and concentrations of plant hormones were applied for direct root induction from juvenile leaf explants of the apical meristem in the offshoot shoot terminal. The highest percentage of induced roots was obtained on the Murashige and Skoog (MS) medium complemented with NAA (1 mg L-1), NOA (3 mg L-1), IBA (1 mg L-1), and 2ip (0.1 mg L-1). The highest percentage of induced callus, both embryogenic and non-embryogenic callus, was achieved from in vitro-induced roots cultured on the Murashige and Skoog medium complemented with BAP (2 mg L-1) and NAA (0.1 mg L-1). The highest number of somatic embryos was acquired on the Murashige and Skoog medium complemented with BAP (2 mg L-1) and NAA (0.1 mg L-1). Successful rooting of date palm plantlets achieved on the PGR-free ½ MS medium following three weeks. The endurance value of rooted plantlets obtained from indirect somatic embryogenesis pathway, was 70.32%. The developed protocol would be applicable for rapid and efficient mass propagation of elite cultivars of date palm with less risk of generating genetic instability.
کلیدواژه‌ها
Phoenix dactylifera L؛ Direct organogenesis؛ Micropropagation؛ Somatic embryos؛ Plant growth regulator
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تعداد مشاهده مقاله: 543
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