|تعداد مشاهده مقاله||108,093,301|
|تعداد دریافت فایل اصل مقاله||84,496,136|
Effect of Phenological Stages on Essential Oil Content, Composition and Rosmarinic Acid in Rosmarinus officinalis L.
|International Journal of Horticultural Science and Technology|
|مقاله 10، دوره 4، شماره 2، اسفند 2017، صفحه 251-258 اصل مقاله (582.31 K)|
|نوع مقاله: Research paper|
|شناسه دیجیتال (DOI): 10.22059/ijhst.2017.234339.194|
|Kianoush Hassanzadeh1؛ Sasan Aliniaeifard* 2؛ Mohammad Mehdi Farzinia3؛ Madeh Ahmadi4|
|1Kian Darou Zagros Co., Khorramabad, Iran.|
|2Department of Horticulture, Aburaihan Campus, University of Tehran, PC. 3391653775, Pakdasht, Tehran, Iran|
|3Department of Horticulture, Baharan institute of Natural Sciences, Gorgan, Iran.|
|4Department of Horticulture Science, Gorgan University of Agricultural Sciences and Natural Resources, Iran.|
|Rosemary (Rosmarinus officinalis L.) is an important medicinal plant that contained a wide range of secondary metabolites such as rosmarinic acid. In this study, essential oil content and composition and rosmarinc acid were determined at three phenological stages (before flowering, full flowering and fruit set stages). Hydro distillation method was used for essential oil extraction and GC and GC/MS was used for essential oil composition. On the basis of the obtained results rosemary plants in full flowering stage had higher amount of essential oil (1.99 ml/100 g dry weight) than in before flowering and fruit set stages (1.2 and 1.01 ml/100 per g dry weight, respectively). The extracted essential oil was contained 1,8-cineole, α-pinene, verbenone, camphor, geraniol, borneol acetae, camphene and linalool as major constituents. These constituents were affected by phenological stages. Before flowering, 1,8-cineole, α-pinene and verbenone (13.68%), in full flowering, 1,8-cineole (17.90%) and in fruit set α-pinene (21.77%) were the main constituents of the reosemary essential oil. Using HPLC analysis on leaf extract showed that the highest amount of rosmarinic acid (25.92mg/g DW) was observed in the fruit set stage. In conclusion, for essential oil content full flowering stage can be recommended for harvesting of Rosmarinus officinalis L. and for rosmarinic acid, fruit set stage can be the best time for harvesting of this medicinal plant.|
|Essential oil constituents؛ growth stages؛ phonological stage؛ Rosemary|
Aliniaeifard S, Rezaei-Nejad A, Seifi-Kalhor M, Shahlaei A, Aliniaeifard A. 2010. Comparison of soil and perlite (with nutrient solution supply) growing media for cultivation of lemon verbena (Lippia citriodora var. ‘Verbena’). Medicinal and Aromatic Plant Science and Biotechnology 4, 30-33.
Almela L, Sachez B, Fernandez J.A, Roca M, Rabe V. (2006). Liquid chromatographic mass spectrometric analysis of phenolics and radical scavenging activity of rosemary extract from different raw material. Journal of Chromatography A. 1120(1), 221-229.
Angelov P, Condoret, J.S. 2007. Optimization of operational conditions of ethanol extraction of Rosmarinic acid from lemon balm (Melissa officinalis L.). Chemistry book 5. University of Plovdiv, Bulgaria, Pp. 71-76.
Anusuya C, Manoharan S. 2011. Antitumor initiating potential of rosmarinicacid in 7, 12-dimethylbenz(a)anthracene-induced hamster buccal pouch car-cinogenesis. Journal of Environmental Pathology, Toxicology and Oncology 30, 199-211.
Anwar, F. 2009. Changes in composition and antioxidant and antimicrobial activities of essential oil of fennel (Foeniculum vulgare Mill.) fruit at different stages of maturity. Journal Herbs, spices & Medicinal plants 15, 1-16.
Azizi M. 2008. Change in content and chemical composition of Hypericum perforatum L. oil at three harvest. Journal of Herbs, Spices & Medicinal Plants 13, 79-85.
Barnes J, Anderson L.A, Phillipson J.D. 2007. Herbal Medicines. Pharmaceutical press. 710p.
Bicchi C, Binello A, Rubinol P. 2000. Determination of phenolic diterpene antioxidants in rosemary (Rosmarinus Officinalis L.) with different methods of extraction and analysis. Phytochemical Analysis 11(4), 236-242.
El Omri A, Han J.P, Kawada K, Abdrabbah M.B, Isoda H. 2010. Rosmarinus officinalis polyphenols activate cholinergic activitie sin PC12 cells through phosphorylation of ERK1/2. Journal of Ethnopharmacology 131, 451-458.
Emadi F, Yassa N. 2007. Analysis of R. officinalis essential oil at different time. Abstract book of the 3th congress of Medicinal plants. Shaheh University, Tehran, Iran 5-18.
Golparvar A.R. 2011. Determination of the best harvesting times to obtain maximum dry herbage, essential oil and thymol yield in garden thyme (Thymus Vulgaris L.). International Journal of Life Science and Medical Research 1, 1-4.
Hassanzadeh K, Rahimi Tanha S, Farzinia M.M, Ghasemnezhad A. 2014. A comparison of some phenolic compounds and essential oil contents in bacterial-gall contaminated and non-contaminated Rosemary (Rosmarinus officinalis) plants. Journal of Ethno-pharmaceutical products 1(2), 55-60.
Kiadas III, Kotet. 1984. Hungarian pharmacopoeia. VII medicinal konyvkiado.
Lamien-Meda A, Nell M, Lohwasser U, Börner A, Franz C, and Novak J. 2010. Investigation of antioxidant and rosmarinic acid variation in the Sage collection of the genebank in Gatersleben. Journal of Agricultural and Food Chemistry 58, 3813–3819.
Martinov M, Oztekin S, Muller J. 2007. Medicinal and Aromatic Crops: Harvesting, Drying, and Processing. CRC Press, United States of America. 320p.
McCue P.P, Shetty K. 2004. Inhibitory effects of rosmarinic acid extracts on porcine pancreatic amylase in vitro. Asia Pacific Journal of Clinical Nutrition 13(1), 101-106.
McGimpsey J.A. 2006. Seasonal variation in essential oil yield and composition from naturalize Thymus vulgaris L. in New Zealand. Flavour and Fragrance Journal 347-352.
Omidbaigi R. 2010. Approaches to Production and Processing of Medicinal plants. Behnashr Press, Mashhad, Iran. 4, 423.
Petersen M, Abdullah Y, Benner J, Eberle D, Gehlen K, Hucherig S, Janiak V, Kim K.H, Sander M, Weitzel C, Wolters S. 2009. Evolution of rosmarinic acid biosynthesis. Phytochemistery 70, 1663-1679.
Pintore G, Usai M, Bradesi P, Juliano C, Batto G, Tomi F, Chessa M, Cerri R, Casanova J. 2002. Chemical composition and antimicrobial activity of Rosmarinus Officinalis L. oils from sardinia and corsica. Flavour and Fragrance Journal 17(1), 15-19.
Ramawat, K.G, Merillon, J.M. 2008. Bioactive molecules and medicinal plants. Springer prees. 379p.
Ramezani S, Rasouli F, Solaimani B. 2009. Changes in essential oil content of Coriander (Coriandrum sativum L.) aerial parts during four phonological stages in Iran. Journal of essential oil bearing plants (12)6, 683-689.
Saeb K, Gholamrezaee S. 2012. Variation of essential oil composition of Melissa officinalis L. leaves during different stages of plant growth. Asian Pacific Journal of Tropical Biomedicine 2(2), S547-S549.
Swarup V, Ghosh J, Ghosh S, Saxena A, Basu A. 2007. Antiviral and anti-inflammatory effects of rosmarinic acid in an experimental murine modelof Japanese encephalitis. Antimicrobial Agents and Chemotherapy 51, 3367-3370.
Tepe B. 2008. Antioxidant potentials and rosmarinic acid levels of the methano-lic extracts of Salvia virgata (Jacq) Salvia staminea (Montbret and Aucher exBentham) and Salvia verbenaca (L.) from Turkey. Bioresource Technology 99, 1584-1588.
Wada M, Kido H, Ohyama K, Kishikawa N, Ohba Y, Kuroda N, Naka Shima K. 2004. Evaluation of quenching effects of non-water-soluble and water-soluble rosemary extracts against active oxygen species by chemiluminescent assay. Food Chemistry 87(2), 261-267.
تعداد مشاهده مقاله: 688
تعداد دریافت فایل اصل مقاله: 486