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بهینه سازی تولید بیوپلیمر پولولان توسط Aureobasidium pullulans از ضایعات انار | ||
مهندسی بیوسیستم ایران | ||
مقاله 9، دوره 48، شماره 3، مهر 1396، صفحه 319-327 اصل مقاله (671.25 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/ijbse.2017.232470.664935 | ||
نویسندگان | ||
حمیرا میرزایی1؛ فرامرز خدائیان* 2؛ محمد جوان نیکخواه3 | ||
1دانشجو کارشناسی ارشد علوم و مهندسی صنایع غذایی | ||
2دانشیار، گروه علوم و مهندسی صنایع غذیی ، پردیس کشاورزی و منابع طبی,ی ، دانشگاه تهران | ||
3استاد گروه گیاه پزشکی، پردیس کشاورزی و منابع طبیعی دانشگاه تهران، کرج | ||
چکیده | ||
در این مطالعه بهینه سازی شرایط تخمیر جهت تولید پولولان از ضایعات انار توسط Aureobasidium pullulans (با شماره دستیابی KY767023 ) مورد بررسی قرار گرفت. روش بهینهیابی سطح پاسخ [a1] به منظور بررسی اثرات سه عامل pH اولیه، درصد تلقیح و نسبت رطوبت اولیه به سوبسترا بر میزان تولید پولولان در محیط سوبسترای جامد (SSF) مورد استفاده قرار گرفت. نتایج این آزمایشات نشان داد که حداکثر تولید پولولان در شرایط بهینه (pH اولیه: 75/7، درصد تلقیح: %5 حجمی/حجمی و نسبت رطوبت اولیه به سوبسترا: 5/2) g/kg 35/0 ± 5/50 است. بررسی ویژگیهای فیزیکوشیمیایی پولولان به دست آمده در شرایط بهینه نیز مورد مطالعه قرار گرفت. فعالیت امولسیفایری پولولان استخراجی 63 درصد و پایداری امولسیون محلول پولولان به ترتیب در دمای °C 4 و °C 25 پس از 30 روز نگهداری، 5/60 و 5/58 درصد گزارش شد. نتایج حاصل از اندازه گیری ویسکوزیته در غلظت های مختلف نشان داد که محلول پولولان در غلظتهای پایین (5/0-1/0 درصد حجمی/ وزنی) دارای رفتار جریان نزدیک به نیوتنی بوده و با افزایش غلظت محلول، از نیوتنی به رقیق شونده با برش تغییر رفتار میدهد. بررسی طیف سنجی FT-IR پولولان بدست آمده از ضایعات انار حضور پیوندهای (6→1) α را در واحدهای مالتوتریوز تایید میکند. | ||
کلیدواژهها | ||
پولولان؛ ضایعات انار؛ روش سطح پاسخ؛ درصد تلقیح؛ FT-IR | ||
مراجع | ||
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