Papizadeh, Moslem, Roayaei Ardakani, Mohammad, Motamedi, Hossein. (1395). Growth-phase dependent biodesulfurization of Dibenzothiophene by Enterobacter sp. strain NISOC-03. , 3(1), 101-111. doi: 10.22059/poll.2017.59576
Moslem Papizadeh; Mohammad Roayaei Ardakani; Hossein Motamedi. "Growth-phase dependent biodesulfurization of Dibenzothiophene by Enterobacter sp. strain NISOC-03". , 3, 1, 1395, 101-111. doi: 10.22059/poll.2017.59576
Papizadeh, Moslem, Roayaei Ardakani, Mohammad, Motamedi, Hossein. (1395). 'Growth-phase dependent biodesulfurization of Dibenzothiophene by Enterobacter sp. strain NISOC-03', , 3(1), pp. 101-111. doi: 10.22059/poll.2017.59576
Papizadeh, Moslem, Roayaei Ardakani, Mohammad, Motamedi, Hossein. Growth-phase dependent biodesulfurization of Dibenzothiophene by Enterobacter sp. strain NISOC-03. , 1395; 3(1): 101-111. doi: 10.22059/poll.2017.59576

Growth-phase dependent biodesulfurization of Dibenzothiophene by Enterobacter sp. strain NISOC-03

Pollution
مقاله 10، دوره 3، شماره 1، 2017، صفحه 101-111    اصل مقاله (454.14 K)
نوع مقاله: Original Research Paper
شناسه دیجیتال (DOI): 10.22059/poll.2017.59576
نویسندگان
Moslem Papizadeh* 1، 2؛ Mohammad Roayaei Ardakani3، 4؛ Hossein Motamedi4
1Microorganisms Bank, Iranian Biological Resource Center (IBRC), Academic Center for Education, Culture and Research (ACECR), Tehran, Iran
2Microbiology Research Center, Pasteur Institute of Iran, Tehran, Iran
3Biotechnology and Biology Research Center, Shahid Chamran University of Ahvaz, Ahvaz, Iran
4Department of Biology, Faculty of Science, Shahid Chamran University of Ahvaz, Ahvaz, Iran
چکیده
Petroleum-polluted soil samples from Ahvaz oilfield were enriched, using three methods to detect microorganisms with different dibenzothiophene degradation capabilities. Strain NISOC-03, a nitrate-reducing, oxidase negative, catalase, citrate, and urease positive, gram negative rod, showed interesting dibenzothiophene desulfurization behavior, designated as Entreobacter sp. strain NISOC-03 based on phenotype and genotype analyses. Gas chromatography, biomass measurement, and Gibb’s assay showed that in the presence of benzoate as the carbon source, strain NISOC-03 utilized 64% of 0.8 mM dibenzothiophene, producing 0.27 mM phenyl phenol during the exponential growth phase, though the produced phenyl phenol was degraded in the stationary growth phase. In the presence of glucose as the carbon source, however, strain NISOC-03 metabolized only 19.6% of 0.8 mM dibenzothiophene. Furthermore, replacing glucose with ethanol or glycerol led to the same reduction of the dibenzothiophene utilization. It is thus concluded that the chemistry of the potential carbon source(s) in the culture medium has a significant influence on the quality and the rate of dibenzothiophene metablization, and the enrichment designation has a very vital effect on the biodegradation efficiency of the isolated microorganisms.
کلیدواژه‌ها
biodegradation؛ biodesulfurization؛ dibenzothiophene؛ enterobacter؛ phenyl phenol
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