سامانه پشتیبانی خدمات اطلاعات

  اخبار و اعلانات

 آمار

تعداد نشریات161
تعداد شماره‌ها6,532
تعداد مقالات70,501
تعداد مشاهده مقاله124,114,323
تعداد دریافت فایل اصل مقاله97,218,115
Jabbari, mohammad reza, Sabahi, Farzaneh, Khansarinejad, Behzad, Shirkoohi, Reza, Saberi, Hooshang, Parvin, Mahmoud, Ahmadi, Elham. (1395). Human cytomegalovirus infection in tumor specimens of Iranian patients with glioma. , 6(1), 11-18. doi: 10.22059/pbs.2016.59003
mohammad reza Jabbari; Farzaneh Sabahi; Behzad Khansarinejad; Reza Shirkoohi; Hooshang Saberi; Mahmoud Parvin; Elham Ahmadi. "Human cytomegalovirus infection in tumor specimens of Iranian patients with glioma". , 6, 1, 1395, 11-18. doi: 10.22059/pbs.2016.59003
Jabbari, mohammad reza, Sabahi, Farzaneh, Khansarinejad, Behzad, Shirkoohi, Reza, Saberi, Hooshang, Parvin, Mahmoud, Ahmadi, Elham. (1395). 'Human cytomegalovirus infection in tumor specimens of Iranian patients with glioma', , 6(1), pp. 11-18. doi: 10.22059/pbs.2016.59003
Jabbari, mohammad reza, Sabahi, Farzaneh, Khansarinejad, Behzad, Shirkoohi, Reza, Saberi, Hooshang, Parvin, Mahmoud, Ahmadi, Elham. Human cytomegalovirus infection in tumor specimens of Iranian patients with glioma. , 1395; 6(1): 11-18. doi: 10.22059/pbs.2016.59003

Human cytomegalovirus infection in tumor specimens of Iranian patients with glioma

Progress in Biological Sciences
مقاله 2، دوره 6، شماره 1، شهریور 2016، صفحه 11-18    اصل مقاله (504.25 K)
نوع مقاله: Original Research Papers
شناسه دیجیتال (DOI): 10.22059/pbs.2016.59003
نویسندگان
mohammad reza Jabbari* 1؛ Farzaneh Sabahi2؛ Behzad Khansarinejad3؛ Reza Shirkoohi4؛ Hooshang Saberi5؛ Mahmoud Parvin6؛ Elham Ahmadi2
1PhD student,Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
2Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
3Department of Microbiology and Immunology, Arak University of Medical Sciences, Arak, Iran
4Department of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran
5Department of Neurosurgery, Brain and Spinal Cord Injuries Repair Research Center, Imam Khomeini Hospital, Tehran University of Medical Sciences, Tehran, Iran
6Department of Pathology, Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran,Iran .
چکیده
Human cytomegalovirus (HCMV) causes persistent infection in humans and severe diseases in fetus and
immunocompromised individuals. Although HCMV is not currently implicated in human cancer, emerging evidence suggests that HCMV infection might be specifically associated with some human malignancies including glioma. Glioma is one of the most common brain tumors affecting children and adults. In this study, we used Real-Time (RT) PCR and immunohistochemistry techniques for detection of HCMV infection in glioma brain tumor biopsies. Paraffin embedded tumor tissues were obtained from patients who had been diagnosed with glioma. After designing of specific primers for the HCMV US28 region, a RT-PCR method was developed for HCMV DNA detection. Immunohistochemistry was performed on the same samples by using monoclonal antibodies specific for immediate earlyprotein (IE)-72 and IE 86 protein of HCMV. The results of RT-PCR on 4 of 18 patients (22/2 %) were positive. Two of the patients with HCMV positive RT-PCR results, passed away. Seven patients (38.8%) were positive with the IHC assay. It was also shown that in patients with higher grade of glioma, higher level of positive cells was observed using IE72 and IE 86 antibodies. Considering the results and controversies associated with reports from other regions of the world, a more comprehensive study using this and other diagnostic methods are suggested in Iranian patients with glioma.
کلیدواژه‌ها
Molecular genetics؛ Immunology؛ Immunohistochemistry؛ Antibody؛ IE 86 Antibody؛ Real-Time PCR
آمار
تعداد مشاهده مقاله: 1,380
تعداد دریافت فایل اصل مقاله: 943


سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب