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تأثیر کود‌های زیستی در مقاومت خیار گلخانه‌ای به بیماری مرگ گیاهچه ناشی از Pythium aphanidermatum و افزایش اجزای عملکرد | ||
| کنترل بیولوژیک آفات و بیماری های گیاهی | ||
| مقاله 10، دوره 5، شماره 1، خرداد 1395، صفحه 111-122 اصل مقاله (926.44 K) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/jbioc.2016.58933 | ||
| نویسندگان | ||
| سید کاظم صباغ* 1؛ شاهو ولی زاده2 | ||
| 1دانشیار گروه زیست‌شناسی، دانشکدۀ علوم، دانشگاه یزد | ||
| 2دانشجوی سابق کارشناسی ارشد بیماری‌شناسی گیاهی، گروه گیاه‌پزشکی، دانشگاه زابل | ||
| چکیده | ||
| بیماری مرگ گیاهچۀ خیار Pythium aphanidermatum یکی از بیماری‌های زیانبار خیار است. در این تحقیق، تأثیر سه کود زیستی (بیولوژیک) شامل: نیتروکسین، قارچ‌ریشه (میکوریز) و ورمی‌کمپوست به‌صورت تنها و تلفیق، در القای مقاومت و افزایش اجزای عملکرد خیار گلخانه‌ای (ES-2862) آلوده به بیماری مرگ گیاهچه بررسی شد. این آزمایش در شرایط گلخانه و در قالب طرح کامل تصادفی با چهار تکرار انجام شد. نتایج بررسی شدت بیماریزایی نشان داد که تیمار ورمی‌کمپوست + قارچ‌ریشه+ نیتروکسین (37درصد) و تیمار ورمی‌کمپوست + نیتروکسین (73درصد) بهترتیب بیشترین و کمترین تأثیر را در کاهش شدت بیماری دارند. تیمار مربوط به مخلوط سه کود بیشترین تأثیر را در افزایش اجزای عملکرد خیار داشت، درحالی‌که قارچ‌ریشه به‌تنهایی کمترین تأثیر را در اغلب صفات نشان داد. بررسی میزان تغییرپذیری ژن‌های Cupi4 و Chitinase با روش qRT-PCR نشان داد که بیان این دو ژن، تحت تأثیر تیمارها قرارگرفته و برای ژن Cupi4 (65/23) تیمار مخلوط سه کود و برای ژن Chitinase (45/2) تیمار قارچ‌ریشه، بیشترین تأثیر را در الگوی بیان ژن نشان داد. با توجه به نتایج به‌دست‌آمده، چنین نتیجه‌گیری می‌شود که از این کودها می‌توان به‌عنوان عامل‌های بیوکنترل در برابر عامل‌های بیمارگر قارچی استفاده کرد. | ||
| کلیدواژه‌ها | ||
| القای مقاومت؛ بوته‌میری خیار؛ بیان ژن؛ کود‌های زیستی؛ کیتیناز | ||
| عنوان مقاله [English] | ||
| Effect of bio-fertilizers on greenhouse cucumber resistant to damping-off disease caused by Pythium aphanidermatum and increase of yield component | ||
| نویسندگان [English] | ||
| Seyed Kazem Sabbagh1؛ Shahoo Valizadeh2 | ||
| 1Associate Professor, Plant Biotechnology, Department of Biology, Faculty of Science, Yazd University, Iran | ||
| 2Former M.Sc. Student, Plant Pathology, Department of Plant Protection, Faculty of Agriculture, University of Zabol, Iran | ||
| چکیده [English] | ||
| Damping-off disease of cucumber caused by soil-born fungi Pythium aphanidermatum is one of the most destructive diseases of cucumber. In this research, the effect of three bio-fertilizers including; nitroxin, mycorrhiza and vermicompost in single and combined form on induced resistance and yield components in greenhouse cucumber (ES-2862) infected with damping-off disease was investigated. These experiments were performed in a completely randomized design with four repetitions in greenhouse condition. The measurment of disease severity showed that V+N+M (37%) and vermicompost + nitroxin (73%) treatment have the highest and the lowest effects on disease severity reduction, respectively. The mixed treatment of three bio-fertilizers has the best effect on increase of yield components when compared to control. The application of mycorriza alone showed the lowest effect on majority of traits. Gene expression analysis of Cupi4 and Chitinase genes by qRT-PCR showed that expression level of two genes has been affected by bio-fertilizers treatment. For Cupi4 gene (23.65), combination of three bio-fertilizers and for chitinase gene (2.45), mycorrhizal treatment showed the highest effect of expression pattern. Regarding the results, it can be concluded that these bio-fertilizers could be use as bio-control agents to plant improving against pathogenic fungal agents. | ||
| کلیدواژه‌ها [English] | ||
| Bio-fertilizers, chitinas, cucumber damping-off, gene expression, induced resistant | ||
| مراجع | ||
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