Soltani, Mahdi, Moghimi, Seyedeh Mahsa, Ebrahimzade Mousavi, Hoseinali, Abdi, Kazem, Soltani, Elahe. (1395). Isolation, phenotypic and molecular characterization of motile Aeromonas species, the cause of bacterial hemorrhagic septicemia in affected farmed carp in Iran. , 10(3), 209-216. doi: 10.22059/ijvm.2016.58683
Mahdi Soltani; Seyedeh Mahsa Moghimi; Hoseinali Ebrahimzade Mousavi; Kazem Abdi; Elahe Soltani. "Isolation, phenotypic and molecular characterization of motile Aeromonas species, the cause of bacterial hemorrhagic septicemia in affected farmed carp in Iran". , 10, 3, 1395, 209-216. doi: 10.22059/ijvm.2016.58683
Soltani, Mahdi, Moghimi, Seyedeh Mahsa, Ebrahimzade Mousavi, Hoseinali, Abdi, Kazem, Soltani, Elahe. (1395). 'Isolation, phenotypic and molecular characterization of motile Aeromonas species, the cause of bacterial hemorrhagic septicemia in affected farmed carp in Iran', , 10(3), pp. 209-216. doi: 10.22059/ijvm.2016.58683
Soltani, Mahdi, Moghimi, Seyedeh Mahsa, Ebrahimzade Mousavi, Hoseinali, Abdi, Kazem, Soltani, Elahe. Isolation, phenotypic and molecular characterization of motile Aeromonas species, the cause of bacterial hemorrhagic septicemia in affected farmed carp in Iran. , 1395; 10(3): 209-216. doi: 10.22059/ijvm.2016.58683

Isolation, phenotypic and molecular characterization of motile Aeromonas species, the cause of bacterial hemorrhagic septicemia in affected farmed carp in Iran

Iranian Journal of Veterinary Medicine
مقاله 7، دوره 10، شماره 3، 2016، صفحه 209-216    اصل مقاله (1.34 M)
نوع مقاله: Aquatic health and Culture
شناسه دیجیتال (DOI): 10.22059/ijvm.2016.58683
نویسندگان
Mahdi Soltani* 1؛ Seyedeh Mahsa Moghimi2؛ Hoseinali Ebrahimzade Mousavi3؛ Kazem Abdi4؛ Elahe Soltani5
11Department of Aquatic Animal Health, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2PhD Candidate, Department of aquatic animal health, Faculty of veterinary medicine, University of Tehran
3Full professor, Department of aquatic animal health, Faculty of veterinary medicine, University of Tehran
4Assistant professor, Aquatic animal health expert, Directorate of health and control of aquatic animal diseases, Iranian veterinary organization
5Department of microbiology, faculty of science, university of Tehran
چکیده
Background: Motile Aeromonas species cause heavy
mortalities in carp farms during spring and summer in Iran. Objectives: The aim of this study was to detect phenotypic
and genotypic characterization of motile Aeromonas species isolated from
diseased carps in some northern and southern provinces of Iran. Methods: A number of 40 samples from 22 fish farms were
collected. The identified motile Aeromonas species were sequenced and
phylogenetic tree was drawn by MEGA6 using UPGMA analysis. Results: A number of 19 bacterial isolates were
identified as motile Aeromonas sp. by biochemical tests, and the DNA
segments of 16S rRNA gene of all these strains gave 1200 bp after running on 1%
agarose electrophorus gel. Also, the sequencing results showed that the
bacterial samples were determined as A. hydrophila and A. veronii
biovar veronii. Conclusions: Phylogenetic analysis revealed that motile Aeromonas
strains in this study were separated in two clusters and four  genogroups with high similarities
کلیدواژه‌ها
Farmed carp؛ Motile Aeromonas septicemia؛ 16S rRNA gene
مراجع
آمار
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