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بررسی اثر درجۀ حرارت در غلظت کشندة (LC50 96-h) سم سایپرمترین (Cypermethrin) در ماهی سفید دریای خزر (Rutilus frisii kutum, Kamensky, 1901) | ||
شیلات | ||
مقاله 7، دوره 68، شماره 3، مهر 1394، صفحه 421-435 اصل مقاله (661.09 K) | ||
نوع مقاله: مقاله پژوهشی | ||
شناسه دیجیتال (DOI): 10.22059/jfisheries.2015.56121 | ||
نویسندگان | ||
سعید شهبازی1؛ علیرضا میرواقفی* 2؛ مجید عابدی1؛ مهدی طاهریان1 | ||
1کارشناس ارشد، گروه شیلات، دانشکدة منابع طبیعی دانشگاه تهران | ||
2دانشیار گروه شیلات، دانشکدة منابع طبیعی دانشگاه تهران | ||
چکیده | ||
طی این تحقیق سمیت حاد[1] حشرهکش سایپرمترین در سه سطح دمایی مختلف (15 و 20 و 25 درجۀ سانتیگراد) در بچهماهیان 1±3 گرمی (میانگین± SD) سفیدماهی دریای خزر (Rutilus frisii kutum Kamensky, 1901) به منظور تعیین غلظت کشندة 50 درصد از جمعیت ماهیان در 96 ساعت مطالعه و بررسی شد. آزمایشها به صورت ساکن[2] و بر اساس روش استانداردO.E.C.D به مدت 4 شبانه روز (96ساعت) انجام و پارامترهای مؤثر فیزیکوشیمیایی آب از جمله pH، سختی کل، اکسیژن محلول و درجۀ حرارت کنترل شد. آزمایشهای ابتدایی به منظور یافتن محدودة کشندگی با رهاسازی بچهماهیان به داخل آکواریومهایی که به حجم 20 لیتر آبگیری و به طور مداوم هوادهی میشدند به تعداد 10 عدد بچهماهی برای هر آکواریوم انجام شد و پس از بهدستآمدن محدودة کشندگی تیمارهای نهایی با 6 تیمار و 3 تکرار مشخص شد و در نهایت بر اساس نتایج و با استفاده از آنالیز پروبیت، مقادیرLC10 ، LC30، LC50، LC70، LC90 و LC99 در 24، 48، 72 و 96 ساعت برای هر سه سطح دمایی بر روی سفیدماهی اندازهگیری شد و سمیت حاد (LC50 96-h) سم سایپرمترین برای بچهماهی سفید در دماهای ºC 1±15، ºC1±20 و ºC 1±25 به ترتیب برابر54/1، 49/1 و 30/1 میکروگرم در لیتر به دست آمد. نتایج نشان داد با افزایش درجۀ حرارت سمیت سایپرمترین افزایش مییابد و مرگومیر بیشتری برای ماهیان اتفاق میافتد. در این آزمایش از نظر بالینی علائمی مانند خونمردگی در ناحیة ساقة دمی، تنفس غیرعادی، شنای نامنظم در ماهیان مورد آزمایش مشاهده شد. | ||
کلیدواژهها | ||
حشرهکش؛ درجۀ حرارت؛ سایپرمترین؛ سفیدماهی (Rutilus frisii kutum)؛ LC50 96h | ||
مراجع | ||
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