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حاج صمدی, زهرا, موحدی فاضل, مرتضی, کاوسی, اورنگ, فتوحی, کبری. (1394). تجمع بیولوژیکی برخی از منابع انرژی در سن گندم Eurygaster integriceps (Hem. Scutelleridae) تحت تیمار نیم‌آزال. , 46(1), 19-31. doi: 10.22059/ijpps.2015.54715
زهرا حاج صمدی; مرتضی موحدی فاضل; اورنگ کاوسی; کبری فتوحی. "تجمع بیولوژیکی برخی از منابع انرژی در سن گندم Eurygaster integriceps (Hem. Scutelleridae) تحت تیمار نیم‌آزال". , 46, 1, 1394, 19-31. doi: 10.22059/ijpps.2015.54715
حاج صمدی, زهرا, موحدی فاضل, مرتضی, کاوسی, اورنگ, فتوحی, کبری. (1394). 'تجمع بیولوژیکی برخی از منابع انرژی در سن گندم Eurygaster integriceps (Hem. Scutelleridae) تحت تیمار نیم‌آزال', , 46(1), pp. 19-31. doi: 10.22059/ijpps.2015.54715
حاج صمدی, زهرا, موحدی فاضل, مرتضی, کاوسی, اورنگ, فتوحی, کبری. تجمع بیولوژیکی برخی از منابع انرژی در سن گندم Eurygaster integriceps (Hem. Scutelleridae) تحت تیمار نیم‌آزال. , 1394; 46(1): 19-31. doi: 10.22059/ijpps.2015.54715

تجمع بیولوژیکی برخی از منابع انرژی در سن گندم Eurygaster integriceps (Hem. Scutelleridae) تحت تیمار نیم‌آزال

دانش گیاهپزشکی ایران
مقاله 4، دوره 46، شماره 1، فروردین 1394، صفحه 19-31    اصل مقاله (506.73 K)
نوع مقاله: مقاله پژوهشی
شناسه دیجیتال (DOI): 10.22059/ijpps.2015.54715
نویسندگان
زهرا حاج صمدی1؛ مرتضی موحدی فاضل* 2؛ اورنگ کاوسی2؛ کبری فتوحی1
1دانشجوی سابق کارشناسی ارشد، حشره‌شناسی، دانشکدۀ کشاورزی، دانشگاه زنجان
2استادیار، گروه گیاه‌پزشکی، دانشکدۀ کشاورزی، دانشگاه زنجان
چکیده
ذخیرۀ منابع انرژی در حشرات کامل زمستان‌گذران سن گندم Eurygaster integriceps اهمیت شایان توجهی در بقای زمستانی آنها دارد. در پژوهش حاضر، تغییر ترکیبات بیوشیمیایی حشرات کامل نسل جدید سن گندم تیمارشده با غلظت‌های زیرکشندۀ نیم‌آزال تی‌اس یک درصد مطالعه شد. نخست، حشرات کامل سن گندم با غلظت‌های مختلف نیم‌آزال (0، 100، 300 و 500 میکرولیتر بر لیتر (μl/l)) تحت شرایط مزرعه تیمار شدند و نمونه‌برداری به فواصل زمانی 3، 6 و 12 روز بعد از سم‌پاشی انجام گرفت. در مرحلۀ دوم، یک هفته پس از سم‌پاشی اول، تعدادی از حشرات مرحلۀ اول مجدداً با غلظت‌های مشابه تیمار شد و نمونه‌برداری 3، 6 و 12 روز بعد انجام گرفت. میزان قند، گلیکوژن، چربی و پروتئین موجود برای هر یک از دو جنس نر و ماده در چهار تکرار بر حسب میلی‌گرم بر گرم وزن تر بدن حشره تعیین شد. نتایج نشان داد که نیم‌آزال تأثیر معناداری بر میزان چربی، گلیکوژن، قند، پروتئین و محتوای کل ذخایر انرژی (001/0P<) دارد و هر سه غلظت سبب افزایش میزان چربی و پروتئین در مقایسه با شاهد شده‌اند. به‌طوری که پس از کسر اثر شاهد، چربی 53/85، 3/97 و 22/108 درصد و پروتئین 62/4، 23/14 و 27/34 درصد به‌ترتیب در غلظت‌های 100، 300 و 500 μl/l افزایش یافته‌اند. میزان گلیکوژن در اولین غلظت به میزان 35/27 درصد کاهش داشته و در بقیۀ غلظت‌ها تفاوت معناداری را با شاهد نشان نداده است. همچنین میزان قند در مقایسه با شاهد به‌ترتیب در سه غلظت 9/9، 37/27 و 76/43 درصد کاهش یافته است. در مجموع محتوای کل ذخایر انرژی در سه غلظت تأثیرات افزایشی نشان داده و گذر زمان و نیز سم‌پاشی مجدد این افزایش را تشدید کرده است. این فاکتور بین دو جنس نر و ماده تفاوت معناداری نداشته است
کلیدواژه‌ها
پروتئین؛ چربی؛ سن گندم؛ کربوهیدرات؛ نیم‌آزال
عنوان مقاله [English]
Bioaccumulation of some bioenergetics resources in Sunn pest, Eurygaster integriceps (Hem. Scutelleridae), under treatment of Neem-azal
نویسندگان [English]
Zahra Hajsamadi1؛ Morteza Movahedi Fazel2؛ Aurang Kavousi2؛ Kobra Fotouhi1
1Former Graduate Student, Entomology, Faculty of Agriculture, University of Zanjan, Iran
2Former Graduate Student, Entomology, Faculty of Agriculture, University of Zanjan, Iran
چکیده [English]
Stored bioenergetic resources in overwintering adults of sunn pest, Eurygaster integriceps, is important in their hibernation. The present study examined some biochemical changes in the adults treated by sub-lethal concentrations (≤ LC30) of Neem-azal T/S. Initially, adults of new generation were sprayed by different concentrations of Neem-azal (100, 300, 500 and 0 µl/l) under field conditions for 3, 6 and 12 days. Then, the adults of the first experiments were resprayed a week later by Neem-azal at the same concentrations prior to resampling for 3, 6 and 12 days. The amounts of lipid, sugar, glycogen and protein of four males and females were determined (mg/g; w/w). Results revealed significant effects Neem-azal on total lipid, glycogen, sugar, protein and energy content (P< 0.001). So that all of three concentration increased lipid and protein contents versus control hemipterans. The net amount of lipids and proteins were increased in 100, 300 and 500 µl/l levels respectively 85.53%, 97.3%, 108.22% and 4.62%, 14.23%, 34.27%. The glycogen quantity decreased 27.35% in 100 µl/l concentration level and no significant differences in other doses with respect to control. Also sugar quantity was decreased 9.9, 27.37 and 43.76% respectively in 100, 300 and 500 µl/l concentration levels. The energy content (EC) was increased in three doses and this trend conntinued with the passage of time. Respraying also was affected on increasing of EC and adult sexes not significant effect on it.
 
کلیدواژه‌ها [English]
Sunn pest, Neem-azal, Lipids, Carbohydrates, proteins
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