|تعداد مشاهده مقاله||111,631,404|
|تعداد دریافت فایل اصل مقاله||86,251,794|
Detection of cytolethal distending toxin (cdt) Genes in Campylobacter jejuni and Campylobacter coli isolated from the intestinal of commercial broiler chickens, turkey and quail of Iran
|Iranian Journal of Veterinary Medicine|
|مقاله 6، دوره 9، شماره 2، مهر 2015، صفحه 109-116 اصل مقاله (962.25 K)|
|شناسه دیجیتال (DOI): 10.22059/ijvm.2015.54009|
|Roja Shojaei Kavan1؛ Mohammad Hassanzadeh* 1؛ Mohammad Hassan Bozorgmehri Fard1؛ Seyed Ali Pourbakhsh2؛ Afshin Akhondzadeh Basti3؛ Abbas Barin4؛ Iradj Ashrafi5|
|1Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Iran|
|2Razi Vaccine and Serum Research Institute, Karaj, Iran|
|3Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, University of Tehran, Iran|
|4Department of Clinical Pathology, Faculty of Veterinary Medicine, University of Tehran, Iran|
|5Department of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Iran|
|Background: Campylobacter jejuni and Campylobacter coli are zoonotic bacteria which are frequently associated with human diarrhea. Sharing of the cytolethal distending toxin (cdt) genes in Campylobacter is common and is considered species specific. OBJECTIVES: In this study we focused on detecting the presence of cdt gene in C. jejuni and C. coli isolated from broilers, turkeys and quails of Iran. METHODS: Cecal samples were randomly collected from 240 broiler chickens, 100 meat type turkeys and 100 quails after slaughtering. We used PCR as a method for detecting cdt genes. RESULTS: In broilers, 93% of 58 C. jejuni positive samples possessed cdt gene and in all cases the three different subunits of cdt genes were present. However, only 56% of 14 C. coli isolates in broilers had contained cdt genes, while one fourth having all three subunits present. In turkeys, around 65% of 34 C. jejuni positive samples had cdt gene present with 38% possessing all three subunits of cdt genes. But all 5 C. coli isolates had all three subunits cdt gene. In quails, 67% of 30 C. jejuni positive samples were identified by cdt gene, 20% of those possessed all three gene subunits. On the other hand, all 28 C. coli isolates of quails had cdt gene present while 36% of those held all three gene subunits. CONCLUSIONS: Our data is indicating the isolation, culture and cdt PCR amplification approaches in this study seemed to be efficient. However, the presence of different variation of Campylobacter cdt gene types in our sample isolates signifies the necessity of further functional gene studies to elucidate which gene type combinations result in encoding effective toxins. |
|Broilers؛ turkeys؛ quails؛ cdt gene؛ Campylobacter jejuni|
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