تعداد نشریات | 161 |
تعداد شمارهها | 6,573 |
تعداد مقالات | 71,032 |
تعداد مشاهده مقاله | 125,502,181 |
تعداد دریافت فایل اصل مقاله | 98,766,145 |
Detection of cytolethal distending toxin (cdt) genes of Campylobacter Jejuni and Coli in fecal samples of pet birds in Iran | ||
Iranian Journal of Veterinary Medicine | ||
مقاله 8، دوره 9، شماره 1، تیر 2015، صفحه 49-56 اصل مقاله (210.28 K) | ||
شناسه دیجیتال (DOI): 10.22059/ijvm.2015.53233 | ||
نویسندگان | ||
Fateme Ehsannejad؛ Arzhang Sheikholmolooki؛ Mohammad Hassanzadeh* ؛ Roja Shojaei Kavan؛ Mohammad Soltani | ||
Department of Avian Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
چکیده | ||
BACKGROUND: Campylobacteriosis is one of the most common bacterial intestinal disorders of humans in many countries. A wide range of domestic and wild bird species have been identified as natural reservoirs. OBJECTIVES: The aim of this study was to determine the Campylobacter spp from pet birds and screen the determined Campylobacters for presence of virulence cytolethal distending toxin (cdt) genes. METHODS: A total of 660 fecal samples from 32 different species of pet birds were taken and examined for detection of Campylobacter spp. and were investigated for presence of cdt genes. All the samples were collected from clinically healthy birds that were kept in cage, zoological parks, and/ or in zoo of Tehran, the capital of Iran. RESULTS: In total, 20 thermophilic Campylobacter were detected from 8 different avian species. From 20 confirmed Campylobacter spp., 16 samples (80%) were C. jejuni positive and 4 (20%) were C. coli in species-specific PCR test. Furthermore, out of 20 detected Campylobacter, 13 (65%) harbored the various subunits of cdtA, cdtB and cdtC genes, and 7 (35%) were negative for all tested cdt genes. CONCLUSIONS: Our findings indicate that the carriage rate of Campylobacter in different species of cage and/or in zoo birds is high and confirm that cdt genes may frequently be present in campylobacter spp. | ||
کلیدواژهها | ||
cdt gens؛ pet birds؛ thermophilic Campylobacter | ||
مراجع | ||
Abuoun, M., Manning, G., Cawthraw, S., Ridley, A., Ahmed, I., Wassenaar, T., Newell, D. (2005) Cytolethal distending toxin (CDT)-negative Campylobacter jejuni strains and anti-CDT neutralizing antibodies are induced during human infection but not during colonization in chickens. Infect Immun. 72: 3053–3062. Abulreesh, H.H., Paget, T.A., Goulder, R. (2006) Campylobacter in waterfowl and aquatic environments: incidence and methods of detection. Environ Sci Technol. 40: 7122–7131. Abulreesh, H.H., Goulder, R. Scott, G.W. (2007) Wild birds and human pathogens in the context of ringing and migration. Ringing and Migration. 33: 193-200. ALLAN, B. (2006) Effect of cytolethal distending toxin of Campylobacter jejuni on adhesion and internalization in cultured cells and in colonization of the chicken gut. Avian Dis. 50: 586–593. Ansari- Lari, M. Hosseinzadeh, S. Shekarforoush. S.S. Abdollahi, M. Berizi, E. (2011) Prevalence and risk factors associated with Campylobacter infections in broiler flocks in Shiraz. Shiraz, southern Iran. Int J Food Microbiol. 144: 475-479. Asakura, M., Samosornsuk, W., Taguchi, M., Kobayashi, K., Misawa, N., Kusumoto, M., Nishimura, K., Matsuhisa, A. Yamasaki, S. (2007) Comparative analysis of cytolethal distending toxin (cdt) genes among Campylobacter jejuni, C. coli, and C. fetus strains. Microb Pathog. 42: 174–183. Asakura, M., Samosornsuk, W., Hinenoya, A., Misawa, N., Nishimura, K., Matsuhisa, A. Yamasaki, S. (2008) Development of a cytolethal distending toxin(cdt) gene-based species-specific multiplex PCR assay for the detection and identification of Campylobacter jejuni, Campylobacter coli and Campylobacter fetus. Immunol Med Microbiol. 52: 260-266. Avrain, L., Humbert, F., LHospitalier, R., Sanders, P., Vernozy-Rozand, C., Kempf, I. (2003) Antimicrobial resistance in Campylobacter from broilers: association with production type and antimicrobial use. Vet Microbiol. 96: 267–276. Blaser, M.J. (1997) Epidemiologic and clinical features of Campylobacter jejuni infections. J Infect Dis. 176: S103–S105. Carter, P.E., McTavish, S.M., Brooks, D., Campbell, J.M., Collins-Emerson, A.C., Midwinter, M. (2009) Novel clonal complexes with an unknown animal reservoir dominate Campylobacter jejuni isolates from river water in New Zealand. Appl Environ Microbiol. 75: 6038–6046. Bang, D., Scheutz, F., Ahrens, P., pedersen, K., Blom, J., Madsen, M. (2001) Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. Isolated from Danish broilers. J Med Microliol. 50: 1087-1094. Findik, A., Ica, T., Emek Onuk, E., Percin, D., Kevenk, T.O., Ciftci, A. (2011) Molecular typing and cdt genes prevalence of Campylobacter jejuni isolates from various sources. Trop Anim Health Prod. 43: 711–719. Hanninen, M., Pajarre, S., Klessner, M., Rauteline, H. (1998) Typing of human Campylobacter jejuni isolates in Finland by pulsedfield gel electrophoresis. J Clin Microbiol. 36: 1787–1789. Hermans, D., Van Deun, K., Martel, A., Van Immerseel, F., Messens, W., Heyndrickx, M., Haesebrouck,. F. Pasmans., F. (2011) Colonization factors of Campylobacter jejuni in the chicken gut. Vet Res. 42:82. Hickey, T., Majam, G., Guerry, P. (2005) Intracelluar survival of Campylobacter jejuni in human monocytic cells and induction of apoptotic death by cytolethal distending toxin. Infect Immun. 73: 5194–5197. Jeffrey, J.S., Atwill, E.R., Hunter, A. (2001) Prevalence of Campylobacter and Salmonella at a squab (Young Pigeon) processing plant. Poul Sci. 80: 151–155. Konkel, M.E., Gray, S.A., Kim, B.J., Garvis, S.G., Yoon, J. (1999) Identification of the enteropathogens Campylobacter jejuni and Campylobacter coli based on the cadF virulence gene and its products. J Clin Microbiol. 37: 510–517. Lutful Kabir, S.M., Kikuchi, K., Asakura, M., Shiramaru, S., Tsuruoka, N., Goto, A., Hinenoya, A., Yamasaki, S.H. (2011) Evaluation of cytolethal distending toxin (cdt) gene-based species-specific multiplex PCR assay for the identification of Campylobacter strain isolated from diarrheal patients in Japan. Jpn J Infect Dis. 64: 19-27. Lara-Tejero, M., Galàn, J.E. (2001) cdtA, cdtB, and cdtC form a tripartite complex that is required for cytolethal distending toxin activity. Infect Immun. 69: 4358–4365. Lara-Tejero, M., Galàn, J.E. (2002) Cytolethal distending toxin: limited damage as a strategy to modulate cellular functions. Trends Microbiol. 10: 147–52. Lee, R., Hassane, D., Cottle, D.L., Pickett, C. (2003) Interactions of Campylobacter jejuni cytolethal distending toxin subunits cdtA and cdtC with HeLa cells. Infect Immun. 71: 4883– 4890. Linton, D., Owen, R.J., Stanley, J. (1996) Rapid identification by PCR of the genus Campylobacter and of five Campylobacter species enteropathogenic for man and animal. Res Microbiol. 147: 707-718. Mullner, P., Jones, G., Noble, A., Spencer, S.E.F., Hathaway, S., French, N.P. (2009) Source attribution of food-borne zoonoses in New Zealand: a modified Hald model. Risk Anal. 29: 970–984. Megraud, F. (1997) Isolation of Campylobacter spp. from Pigeon Feces by a Combined Enrichment- Filtration Technique. Appl Environ Microbiol. 53: 1394-1395 Mirzaie, M., Hassanzadeh, M., Bashashati, M., Barrin, A. (2011) Campylobacter occurrence and antimicrobial resistance in samples from ceca of commercial turkeys and quails in Tehran, Iran. Int Res J Microbiol. 2: 338-342. Newell, D.G. (2001) Animal models of Campylobacter jejuni colonization and disease and the lessons to be learned from similar Helicobacter pylori models. J Appl Microbiol. 90: S57–S67. Newell, D.G., Fearnley, C. (2003) Sources of Campylobacter colonization in broiler chickens. Appl Environ Microbiol. 69: 4343–4351. Newell, D.G., Wagenaar, J.A. (2000) Poultry infections and their control at the farm level, In: Campylobacter. Nachamkin, I., Blaser, M. J. (eds.). (2nd ed.) American Society for Microbiol Press, Washington, D.C., USA. p. 497– 509. Nuijten, P.J., van den Berg, A.J., Formentini, I., van der Zeijst, B.A., Jacobs, A.A. (2000) DNA rearrangements in the flagellin locus of an flaA mutant of Campylobacter jejuni during colonization of chicken ceca. Infect Immunol 68: 7137-7140. Pasquali, F., DE Cesare, A., Manfreda, G., Frahini, A. (2011) Campylobacter control strategies in European poultry production. World’s Poul Sci J. 67: 1-18. On, S.L.W., Holmes, B. (1992) Assessment of enzyme detection tests useful in identification of campylobacteria. J Clin Microbiol. 30: 746- 749. Pickett, C.L., Whitehouse, C.A. (1999) The cytolethal distending toxin family. Trends Microbiol. 7: 292–297. Rahimi, E., Momtaz, H., Bonyadian, M. (2010) PCR detection of Campylobacter spp. from turkey carcasses during processing plant in Iran. Food Control. 21: 692- 694. Rizal, A., Kumar, A., Vidyart, A.S. (2010) Prevalence of pathogenic genes in Campylobacter jejuni isolated from poultry and human. Inter J Food Safet. 12: 29-34. Shane, S.M. (1992) The significance of infection in poultry: a review. Avian Pathol. 21: 189–213. Tamura, K., Peterson, D., Peterson., N., Stecher., G., Nei., M., Kumar, S. (2011) MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol. 31: 1-9. Waldenstrom, J., Broman, T., Carlsson, I., Hasselquist, D., Achterberg, R.P., Wagenaar, J.A., Olsen, B. (2002) Prevalence of Campylobacter jejuni, Campylobacter lari, and Campylobacter coli in different ecological guilds and taxa of migrating birds. Appl Environ Microbiol. 68: 5911–5917. Waldenstrom, J., Mevius, D., Veldman, K., Broman, T., Hasselquist, D., Olsen, B. (2005) Antimicrobial resistance profiles of Campylobacter jejuni isolates from wild birds in Sweden. Appl Environ Microbiol. 71: 2438–2441. Yogasundram, K., Shane, S.M., Harrington, K.S. (1989) Prevalence of Campylobacter jejuni in selected domestic and wild birds in Louisiana. Avian Dis. 33: 664–667. | ||
آمار تعداد مشاهده مقاله: 2,511 تعداد دریافت فایل اصل مقاله: 2,133 |