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Phylogenetic study based on the phosphoprotein gene of Iranian Newcastle disease viruses (NDV) isolates, 2010 -2012 | ||
Iranian Journal of Veterinary Medicine | ||
مقاله 1، دوره 8، شماره 2، آبان 2014، صفحه 73-77 اصل مقاله (116.61 K) | ||
شناسه دیجیتال (DOI): 10.22059/ijvm.2014.51403 | ||
نویسندگان | ||
Arash Ghalyanchi Langeroudi* 1؛ Hossein Hossein2؛ Vahid Karimi3؛ Omid Madadgar1؛ Masoud Hashemzadeh4؛ Seyed Ali Ghafouri5؛ Seyed Sina Bagheri6؛ Seyed Milad Vahedi6 | ||
1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Karaj, Iran | ||
3Department of Poultry Diseases, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
4Department of Research & Production of Veterinary and Poultry Vaccines, Razi Vaccine and Serum Research Institute, Karaj, Iran | ||
5Department of Poultry Health and Diseases, Iranian Veterinary Organization, Tehran, Iran | ||
6Students' Scientific Association of Microbiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran | ||
چکیده | ||
BACKGROUND: Newcastle disease virus (NDV) is the causative agent of the Newcastle disease (ND), a highly contagious disease in birds that causes significant economic losses to the poultry industry worldwide. ND is endemic in Iran and outbreaks are reported regularly in commercial poultry flocks and different species of birds. OBJECTIVES:The current study was carried out to characterize NDV based on phosphorprotein (P) gene from recent outbreaks in Iran, 2010-2012. METHODS: The P gene fragment of NDV isolates of five chickens, 1 ostrich, and 1 Pigeon paramyxovirus-1 was obtained by RT-PCR and sequenced. RESULTS: Phylogenetic analysis of sequences revealed that chicken and ostrich NDV isolates were closely related and placed in the genotype VII and Pigeon Paramyxovirus-1 was located in the genotype V. CONCLUSIONS:This is the first report of Phosphoprotein gene sequences of NDV strains isolated in Iran. This study will help us to understand the epidemiology and molecular characteristics of Newcastle disease virus in Iran. | ||
کلیدواژهها | ||
Newcastle disease؛ phosphoprotein؛ Phylogenetic study | ||
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