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Ghalyanchi Langeroudi, Arash, Babkhani, Narges, Zolfaghari, Mohammad Reza, Majidzadeh Arbadili, Keivan, Morovvati, Abbas, Soleimani, Mohammad. (1392). Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011. , 7(3), 207-211. doi: 10.22059/ijvm.2013.35972
Arash Ghalyanchi Langeroudi; Narges Babkhani; Mohammad Reza Zolfaghari; Keivan Majidzadeh Arbadili; Abbas Morovvati; Mohammad Soleimani. "Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011". , 7, 3, 1392, 207-211. doi: 10.22059/ijvm.2013.35972
Ghalyanchi Langeroudi, Arash, Babkhani, Narges, Zolfaghari, Mohammad Reza, Majidzadeh Arbadili, Keivan, Morovvati, Abbas, Soleimani, Mohammad. (1392). 'Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011', , 7(3), pp. 207-211. doi: 10.22059/ijvm.2013.35972
Ghalyanchi Langeroudi, Arash, Babkhani, Narges, Zolfaghari, Mohammad Reza, Majidzadeh Arbadili, Keivan, Morovvati, Abbas, Soleimani, Mohammad. Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011. , 1392; 7(3): 207-211. doi: 10.22059/ijvm.2013.35972

Detection of Coxeilla brunetii in bulk tank milk samples from dairy bovine farms using nested-PCR in Qom, Iran, 2011

Iranian Journal of Veterinary Medicine
مقاله 7، دوره 7، شماره 3، دی 2013، صفحه 207-211    اصل مقاله (335.39 K)
شناسه دیجیتال (DOI): 10.22059/ijvm.2013.35972
نویسندگان
Arash Ghalyanchi Langeroudi1؛ Narges Babkhani2؛ Mohammad Reza Zolfaghari2؛ Keivan Majidzadeh Arbadili3؛ Abbas Morovvati2؛ Mohammad Soleimani* 4
1Department of Microbiology and Immunology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
3TASNIM Biotechnology Research Center, Faculty of Medicine, AJA University of Medical Sciences, Tehran, Iran
4Department of Microbiology, Qom Branch ,Islamic Azad University, Qom, Iran
چکیده
BACKGROUND: Q fever is a zoonotic disease caused by
Coxiella burnetii, a species of bacteria that is distributed
worldwide. In cattle, Coxiella burnetii infections are generally
asymptomatic but can also be associated with reproductive
disorders. OBJECTIVES: The aim of this study was to achieve
molecular detection of Coxiella burnetii in dairy bovine milk
farms using Nested PCR in Qom province, Iran. METHODS:
From January to February 2011 (winter) and July to September
2011(summer) a total of 100 bovine bulk milk samples were
equally collected from five areas of Qom. The nested PCR assay
used to screen for C. burnetii was designed from the nucleotide
sequence of the com1 gene encodin a 27-kD outer membrane
protein (OMP). RESULTS: In this study, 14% (14 of 100) of bulk
milk were positive. CONCLUSIONS: These results support the
hypothesis of high prevalence and endemic pattern of Q fever in
Qom province of Iran.
کلیدواژه‌ها
Coxeilla brunetii؛ Q fever؛ nested PCR
آمار
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