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Rezaei, Mahdieh, Jamshidi, Shahram, Saffarpour, Anna, Ashouri, Mahdi, Sharifi, Davood, Zamankhan Malayeri, Hamed, Tamimi, Naqa, Rokn, Amir Reza. (1392). Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs). , 7(3), 193-199. doi: 10.22059/ijvm.2013.35970
Mahdieh Rezaei; Shahram Jamshidi; Anna Saffarpour; Mahdi Ashouri; Davood Sharifi; Hamed Zamankhan Malayeri; Naqa Tamimi; Amir Reza Rokn. "Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs)". , 7, 3, 1392, 193-199. doi: 10.22059/ijvm.2013.35970
Rezaei, Mahdieh, Jamshidi, Shahram, Saffarpour, Anna, Ashouri, Mahdi, Sharifi, Davood, Zamankhan Malayeri, Hamed, Tamimi, Naqa, Rokn, Amir Reza. (1392). 'Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs)', , 7(3), pp. 193-199. doi: 10.22059/ijvm.2013.35970
Rezaei, Mahdieh, Jamshidi, Shahram, Saffarpour, Anna, Ashouri, Mahdi, Sharifi, Davood, Zamankhan Malayeri, Hamed, Tamimi, Naqa, Rokn, Amir Reza. Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs). , 1392; 7(3): 193-199. doi: 10.22059/ijvm.2013.35970

Isolation, characterization and transduction of canine bone marrow-derived mesenchymal stem cells (cBM-MSCs)

Iranian Journal of Veterinary Medicine
مقاله 5، دوره 7، شماره 3، دی 2013، صفحه 193-199    اصل مقاله (2.48 M)
شناسه دیجیتال (DOI): 10.22059/ijvm.2013.35970
نویسندگان
Mahdieh Rezaei1؛ Shahram Jamshidi1؛ Anna Saffarpour2؛ Mahdi Ashouri3؛ Davood Sharifi4؛ Hamed Zamankhan Malayeri1؛ Naqa Tamimi1؛ Amir Reza Rokn* 5
1Department of Internal Medicine, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Periodontology, International Campus School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
3Department of Oral and Maxillofacial Pathology, Faculty of Dentistry, Shahed University of Medical Sciences, Tehran, Iran
4Department of Surgery and Radiology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
5Dental Implant Research Center and Department of Periodontics, School of Dentistry, Tehran University of Medical Sciences, Tehran, Iran
چکیده
BACKGROUND: Stem cell therapy in small animal medicine
is still in its infancy and few in vitro and in vivo research projects
regarding animal Mesenchymal Stem Cells (MSCs) have been
carried out. On the other hand, Cell tracking is the first step of the
cell-based therapies and is essential to recognize cell fate post
transplantation. OBJECTIVES: The aim of this study was to
isolate, characterize, and transduce cBM-MSCs. METHODS:
Canine Bone Marrow-derived Mesenchymal Stem Cells (cBMMSCs)
were isolated from bone marrow of dogs and
characterized based on morphology, differentiation capacities,
and surface marker expressions. For the first time, we labeled
cBM-MSCs by GFP-encoding lentiviral vector to track them.
RESULTS: cBM-MSCs were successfully isolated and proliferated.
Morphologically, these cells were similar to other
MSCs from other sources and species and were able to
differentiate into osteocytes and adipocytes. cBM-MSCs
expressed surface marker CD44 but were not able to express
CD34. Approximately, 70% of cells efficaciously expressed
GFPafter labeling; CONCLUSIONS:We found that GFP labeling
is an easy and effective technique to track transplanted cBMMSCs.
Our results also provide fundamental information about
canine BM-MSCs in order to use in veterinary medicine.
کلیدواژه‌ها
canine Bone Marrow-derived Mesenchymal Stem Cells (cBMMSCs)؛ Characterization؛ green fluorescent protein (GFP)؛ transduction
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