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Karimi Farsad, Laleh, Mardi, Mohsen, Ebrahimi, Mohammad Ali. (1391). Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.). , 3(1), 72-78. doi: 10.22059/pbs.2013.32095
Laleh Karimi Farsad; Mohsen Mardi; Mohammad Ali Ebrahimi. "Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.)". , 3, 1, 1391, 72-78. doi: 10.22059/pbs.2013.32095
Karimi Farsad, Laleh, Mardi, Mohsen, Ebrahimi, Mohammad Ali. (1391). 'Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.)', , 3(1), pp. 72-78. doi: 10.22059/pbs.2013.32095
Karimi Farsad, Laleh, Mardi, Mohsen, Ebrahimi, Mohammad Ali. Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.). , 1391; 3(1): 72-78. doi: 10.22059/pbs.2013.32095

Quantitative expression analysis of candidate genes for Septoria tritici blotch resistance in wheat (Triticum aestivum L.)

Progress in Biological Sciences
مقاله 10، دوره 3، شماره 1، خرداد 2013، صفحه 72-78    اصل مقاله (520.52 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22059/pbs.2013.32095
نویسندگان
Laleh Karimi Farsad1؛ Mohsen Mardi* 1؛ Mohammad Ali Ebrahimi2
1Genomics Department, Agricultural Biotechnology Research Institute of Iran (ABRII), Karaj, Iran
2Department of Biotechnology, Payame Noor University, Tehran, Iran.
چکیده
Septoria tritici blotch (STB), caused by the ascomycete fungus Mycosphaerella graminicola (asexual stage: Septoria tritici), is one of the most important foliar diseases of wheat. In this research, quantitative expression analysis of five candidate genes for the induction of resistance to STB (PR-1, Bsi, Msr, Per, and Ppi) was conducted in the wheat cultivars ‘Seri 82’ (susceptible) and ‘Frontana’ (resistant). The study used a randomized complete block design with three replications and five time-points (0, 3, 6, 12, and 24hours after inoculation). Evaluation of the expression of five candidate genes by real-time PCR showed that expression of Per, pr-1, Bsi, Msr genes was higher than 5, 3, 3 and 2 fold in treatment vs. control samples in ‘Frontana’ at 12 hours after inoculation respectively. The results indicated the effectiveness of these genes in the conferral of resistance to STB in wheat.
کلیدواژه‌ها
Septoria tritici؛ Wheat؛ Real-time PCR analysis؛ Resistance gene
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