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Nasri, Sirwan, Bahramnejad, Bahman. (1391). Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros. , 3(1), 60-66. doi: 10.22059/pbs.2013.32093
Sirwan Nasri; Bahman Bahramnejad. "Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros". , 3, 1, 1391, 60-66. doi: 10.22059/pbs.2013.32093
Nasri, Sirwan, Bahramnejad, Bahman. (1391). 'Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros', , 3(1), pp. 60-66. doi: 10.22059/pbs.2013.32093
Nasri, Sirwan, Bahramnejad, Bahman. Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros. , 1391; 3(1): 60-66. doi: 10.22059/pbs.2013.32093

Regeneration and Agrobacterium-mediated transformation of three economically important strawberry cultivars Kurdistan, Camarosa and Paros

Progress in Biological Sciences
مقاله 8، دوره 3، شماره 1، خرداد 2013، صفحه 60-66    اصل مقاله (513.57 K)
نوع مقاله: Research Paper
شناسه دیجیتال (DOI): 10.22059/pbs.2013.32093
نویسندگان
Sirwan Nasri؛ Bahman Bahramnejad
Department of Agricultural Biotechnology, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran
چکیده
Genetic transformation studies were carried out to standardize a protocol for Agrobacterium -mediated genetic transformation of three economically important strawberry (Fragaria x ananassa Duch) cultivars Kurdistan’, Camarosa’, and ‘Paros’. Shoot regeneration frequency 72, 65 and 30% was obtained on MS (1) basal medium supplemented with 2% glucose and 4 mg/l TDZ for Camarosa, Kurdistan and Paros, respectively. To optimize the concentration of kanamycin for these cultivars, we observed the responses of leaf explants to different concentration of kanamycin (0-100 mg/l) in the media. Our results showed that 75 mg/l was an effective concentration of kanamycin. For genetic transformation, Agrobacterium tumefaciens strain C58C1RifR (pGV2260) harbouring the binary vector pTJK136 containing uidA gene with intron along with kanamycin resistance gene (npt-II) was used. After five days pre-incubation and 72 h co-cultivation, transformed cells (explants) were able to grow on the selective regeneration medium containing 75 mg/l kanamycin and 500 mg/l cefotaxime, while, control explants failed to grow. The regenerated putative transgenic shoots were analyzed by histochemical GUS assay and PCR analysis. Transformation efficiency based on inoculated explants number was 2, 1 and 3% for ‘, Camarosa’, ‘Paros ’, and ‘Kurdistan’, respectively. The standardized protocol would be useful for Agrobacterium-mediated genetic transformation of these cultivars with important agronomic genes.
کلیدواژه‌ها
Agrobacterium tumifaciens؛ genetic transformation؛ gus؛ strawberry
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