In vitro Interaction of HSV-1 ORF P with Both Thymidine Kinase (TK) and an Unidentified Cellular Protein

	In vitro Interaction of HSV-1 ORF P with Both Thymidine Kinase (TK) and an Unidentified Cellular Protein
Journal of Sciences, Islamic Republic of Iran
مقاله 2، دوره 17، شماره 1، خرداد 2006 اصل مقاله (152.55 K)
چکیده
Herpes simplex virus type-1 (HSV-1) is a neurotropic pathogen of humans that establishes latent infection in the sensory ganglia innervating the site of primary infection. A number of genes control HSV-1 pathogenicity and latency. Open reading frame P (ORF P) is one of these genes that might have a role in latency and pathogenesis. A complication in the analysis of the role of ORF P in the HSV-1 life-cycle is an antisense overlapping gene, ICP34.5. ORF P is also deleted in ICP34.5 negative mutants and to date, no definite function is attributed to it. An approach to analyze the function of a viral gene such as ORF P is to determine if this gene interacts with any of the cellular and viral proteins both in vitro and in vivo. Therefore, in this work, using GST pulldown assay and Western-blotting, it was investigated that with which cellular and viral gene products ORF P interacts. Our results showed that ORF P interacted with Thymidine kinase (TK) and also with an unidentified cellular protein. Conclusively the results of these works to-gether suggest possible role for ORF P in both splicing and replication of HSV-1.

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