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. (1378). CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI. , 10(2), -.
. "CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI". , 10, 2, 1378, -.
. (1378). 'CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI', , 10(2), pp. -.
. CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI. , 1378; 10(2): -.

CLONING AND EXPRESSION OF A HUMAN INTERFERON a2 GENE IN E. COLI

Journal of Sciences, Islamic Republic of Iran
مقاله 2، دوره 10، شماره 2، شهریور 1999    اصل مقاله (2.14 M)
چکیده
The plasmid pALCA1SIFN containing cDNA that encodes the human interferon
a-2b was obtained from the ATCC(no. 531667). In this system the expression of the
gene is under the control of an alcA promoter. alcA p is a specific promoter for
expression of different genes in Aspergillusfilamentous. In this plasmid the coding
region of IFN?-2b is preceded by the coding region of a synthetic signal peptide. For
direct expression of the IFNa-2b gene under the control of a T7/lac promotor of
pET24d(+) expression vector, three subcloning steps were carried out which resulted
in the construction of 3 new plamids. pHA1, pHA2 and pHA4 in which the IFNa-2
gene is under the control of lacp, lacuv5p and T7 promoter, respectively. Another
plasrnid, pHA3, was also constiucted and is a modified version of pET24d(+).
Provided there is a synthetic signal peptide preceding the coding region of IFNa2
gene, the protein can be seen in either system, as shown by western blotting, albeit
with a different level of expression. The best product can be seen in the pHA4plasmid
with a T7 pas judged by dot and western blotting
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