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PURJFICATION OF HIGH MOLECULAR WEIGHT CELLULOLYTIC ENZYMES FROM CELLUWMONAS sp. STRAIN 0 | ||
Journal of Sciences, Islamic Republic of Iran | ||
مقاله 2، دوره 9، شماره 1، خرداد 1998 اصل مقاله (903.7 K) | ||
چکیده | ||
Purification of extra-cellular cellulolytic enzymes from C e l l u l o mssp. strain 0, isolated from forest soil in the north of Iran , was studied by using gel filtration and ion-exchange chromatography. Two endo-glucanases (Endo-I & Endo-II) and one exo-glucanase (Exo-I) were purified to hornogenity . The purified enzymes had molecular weights of about 39.000,70.000 and 90.000 daltons for Endo-I, Endo-II and Exo-I, respectively. Heavy metal ions, like Fe and CU inactivated the enzymes by about 80%, while Zn and CO had no effect on the enzyme activities | ||
عنوان مقاله [English] | ||
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چکیده [English] | ||
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آمار تعداد مشاهده مقاله: 946 تعداد دریافت فایل اصل مقاله: 973 |