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A NEW METHOD FOR THE QUANTIFICATION OF 1 -(MALONYLAMINO)CYCLOPROPANE- 1 - CARBOXYLIC ACID IN WATER-STRESSED PLANT TISSUES | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 1، دوره 10، شماره 4، اسفند 1999 اصل مقاله (752.68 K) | ||
| چکیده | ||
| Since the discovery of MACC as a major metabolite of both endogenous and exogenously applied ACC, it has become evident that the formation of MACC from ACC can act to regulate ethylene production in certain tissues. Hence, it was suggested that MACC could serve as an indicator of water-stress history in plant tissues. The accurate quantification of MACC in plant tissues is essential for the understanding of the role of MACC in the regulation of ethylene biosynthesis. Hoffman and co-workers described a method for the measurement of MACC in which it was hydrolysed by HC1 to ACC, which was then assayed by chemical oxidation to form ethylene. To date, no other method has been developed for quantifying MACC. Attempts have been made by others to raise monoclonal antibodies to MACC so that an irnrnunoassay could be developed in order to gain a deeper understanding of stress-induced ethylene production. However, to date, no further publications have been forthcoming. Here, quantification methods for MACC , employing GC-MS are described. This method is compared with widely-used indirect assay for MACC, which is based upon hydrolysis of MACC to ACC and conversion of ACC by hypochlorite reagent to ethylene which is subsequently quantified by gas chromatography | ||
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