پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 163 |
| تعداد شماره‌ها | 6,762 |
| تعداد مقالات | 72,833 |
| تعداد مشاهده مقاله | 131,790,102 |
| تعداد دریافت فایل اصل مقاله | 103,504,021 |
A Novel Efficient Method for Extraction and Purification of Recombinant Phenylalanine Dehydrogenase | ||
| Journal of Sciences, Islamic Republic of Iran | ||
| مقاله 5، دوره 19، شماره 1، تیر 2008 اصل مقاله (281.56 K) | ||
| چکیده | ||
| Growing applications of phenylalanine dehydrogenase (PheDH) enzyme in the medical and pharmaceutical industries encourages researchers to seek simple, fast and economical alternative purification methods. With goal of finding a new technique, the extraction and purification of recombinant Bacillus badius PheDH in polyethylene glycol 6000 (PEG-6000) and ammonium sulfate aqueous two-phase systems (ATPS) was studied. Investigation on the effects of PEG molecular weight (MW) and concentration, (NH4)2SO4 concentration, pH, temperature and NaCl addition on partition behavior was also performed. The optimized system was 8.5% (w/w) PEG-6000, 17.5% (w/w) (NH4)2SO4 and 13% (w/w) NaCl at pH 8.0. The specific activity, yield, purification factor, recovery and partition coefficient were obtained 10424.97 U/mg, 95.85%, 474.3, 141% and 92.57, respectively. Briefly, we described the application of ATPS as an interesting and powerful technique for purifying and recovery of recombinant PheDH. | ||
|
آمار تعداد مشاهده مقاله: 887 تعداد دریافت فایل اصل مقاله: 1,354 |
||
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب