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Effect of testosterone on spermatogonial cell colony formation during In vitro co-culture | ||
| Iranian Journal of Veterinary Medicine | ||
| مقاله 4، دوره 6، شماره 3 - شماره پیاپی 1334246، آذر 2012، صفحه 159-164 اصل مقاله (166.46 K) | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2012.30002 | ||
| نویسندگان | ||
| پرویز تاجیک1؛ Parviz Tajik2؛ Mohammad Hossein Yousefi1؛ Mansoreh Movahedin3 | ||
| 1گروه علوم پایه، دانشکده دامپزشکی، دانشگاه سمنان | ||
| 2گروه علوم درمانگاهی، دانشکده دامپزشکی، دانشگاه تهران | ||
| 3گروه علوم تشریحی، دانشکده پزشکی، دانشگاه تربیت مدرس | ||
| چکیده | ||
| Background: The complex process of spermatogenesis is regulated by various factors. Studies on spermatogonial stem cells have provided a very important tool to improve herd genetic and different field. 0.2 to 0.3 percent of total cells of seminiferous tubules consist of spermatogonial stem cells. To investigate and biomanipulate these cells, first the proliferation and viability rate of cells should be increased in vitro. Objectives: In the present study, the in vitro effects of testosterone on spermatogonial cell colony formation were investigated. Methods: Sertoli and spermatogonial cells were isolated from 3-5-month-old calves. The identity of the cells was confirmed through analysis of immuno-cytochemistry. Co-cultured Sertoli and spermatogonial cells were treated with testosterone in different doses of 0.2 µmol L-1, 0.4 µmol L-1 and 0.8 µmol L-1, before colony assay. Results: testosterone did not decrease the proliferation of spermatogonial stem cells. Conclusions: testosterone can be chosen for in vitro colonization of spermatogonial cells with other factors. | ||
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