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نیکبخت بروجنی, غلامرضا, Nikbakht, Gholam Reza, Tajbakhsh, Hasan, Jahantigh, Monireh, Niazi Shahraki, Siavash, Madadgar, Omid. (1391). Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene. , 6(2), 67-71. doi: 10.22059/ijvm.2012.28949
غلامرضا نیکبخت بروجنی; Gholam Reza Nikbakht; Hasan Tajbakhsh; Monireh Jahantigh; Siavash Niazi Shahraki; Omid Madadgar. "Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene". , 6, 2, 1391, 67-71. doi: 10.22059/ijvm.2012.28949
نیکبخت بروجنی, غلامرضا, Nikbakht, Gholam Reza, Tajbakhsh, Hasan, Jahantigh, Monireh, Niazi Shahraki, Siavash, Madadgar, Omid. (1391). 'Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene', , 6(2), pp. 67-71. doi: 10.22059/ijvm.2012.28949
نیکبخت بروجنی, غلامرضا, Nikbakht, Gholam Reza, Tajbakhsh, Hasan, Jahantigh, Monireh, Niazi Shahraki, Siavash, Madadgar, Omid. Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene. , 1391; 6(2): 67-71. doi: 10.22059/ijvm.2012.28949

Development and ELISA-based detection of anti-M2e IgY antibodies using an encoding plasmid for M2e-Hsp70 C-terminal gene

Iranian Journal of Veterinary Medicine
مقاله 1، دوره 6، شماره 2 - شماره پیاپی 1177763، شهریور 2012، صفحه 67-71    اصل مقاله (105.89 K)
شناسه دیجیتال (DOI): 10.22059/ijvm.2012.28949
نویسندگان
غلامرضا نیکبخت بروجنی1؛ Gholam Reza Nikbakht1؛ Hasan Tajbakhsh2؛ Monireh Jahantigh1؛ Siavash Niazi Shahraki3؛ Omid Madadgar1
1گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران
2استاد ممتاز دانشگاه تهران و عضو پیوسته فرهنگستان علوم
3آزمایشگاه مرکز تشخیص سازمان دامپزشکی ایران
چکیده
Background: The use of IgYs in a variety of methods in different areas of research, diagnostics, medical application and biotechnology should be considered widely. Objectives: Development of antibodies against extra cellular domain of influenza M2 (M2e) protein in egg yolk of laying hens. Methods: A Fusion construct harboring C-terminal of bovine heat shock protein 70 (Hsp70) and influenza M2e coding genes was injected to laying hens. Serum and egg yolk antibodies were screened for the presence of anti-M2e antibodies by indirect enzyme-linked immunosorbent assay (ELISA). Results: Anti-M2e antibodies were detected in egg yolks and sera of injected hens from 13 and 7 days post injection (PI), with the peak titer detected on 41 and 35 days PI, respectively. Conclusions: Anti-M2e IgY titers could be an index for expression potential of pcDNA3.1-M2e-HspC-terminal construct in laying hens. This construct could be considered as a promising tool in production of anti-M2e polyclonal, monospecific IgY antibodies. Such anti-M2e antibodies could be exploited for influenza diagnostic and therapeutic measures.
کلیدواژه‌ها
e2M
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