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Sample Preparation for Secretome Analysis in A*-Type Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease | ||
| Progress in Biological Sciences | ||
| مقاله 9، دوره 2، شماره 1، خرداد 2012، صفحه 87-94 اصل مقاله (377.22 K) | ||
| شناسه دیجیتال (DOI): 10.22059/pbs.2012.24828 | ||
| نویسندگان | ||
| Najmeh Mozdoori1؛ Neda Parchami2؛ Nasrin Kazemipour3؛ Seyed Mehdi Alavi2 | ||
| 1National Institute for Genetic Engineering and Biotechnology (NIGEB), P. O. Box: 14155-6343, Tehran, Iran | ||
| 2National Institute for Genetic Engineering and Biotechnology (NIGEB), P. O. Box: 14155-6343, Tehran, Iran | ||
| 3University of Sistan and Baluchestan | ||
| چکیده | ||
| Xanthomonas citri subsp citri (Xcc) is responsible for one of the most destructive diseases of citrus fruits, Asiatic citrus canker. Based on the bacterial host range, the pathogenic forms, A, A*, and Aw have been identified. A*-type strains are not well characterized and they have been reported to differ from other A-type strains on the basis of biochemical and genetic characteristics. In this study, we attempted to develop a medium to mimic in planta conditions for inducing protein secretion of the A*-type strain-NIGEB-088. We also attempted to achieve efficient extraction of Xcc A*-type extracellular proteins, the key mediators of plant-bacterium interaction. Results obtained by this study demonstrate that the m3 (mMM1) can induce Xcc A*-type strains to secret extracellular proteins in amounts detectable by two dimensional gel electrophoresis (2-DE). We also show that Pyrogallol red-molybdate methanol (PRMM) is more effective for precipitation of Xcc A*-type secreted proteins than other assessed procedures of protein precipitation, i. e. acetone, TCA, and sodium deoxycholate | ||
| کلیدواژه‌ها | ||
| Asiatic citrus canker disease؛ in vitro secretome analysis؛ secreted protein extraction؛ Xanthomonas citri subsp. citri | ||
| عنوان مقاله [English] | ||
| Sample Preparation for Secretome Analysis in A*-Type Strains of Xanthomonas citri subsp. citri, the Causal Agent of Asiatic Citrus Canker Disease | ||
| نویسندگان [English] | ||
| Najmeh Mozdoori1؛ Neda Parchami2؛ Nasrin Kazemipour3؛ Seyed Mehdi Alavi2 | ||
| چکیده [English] | ||
| Xanthomonas citri subsp citri (Xcc) is responsible for one of the most destructive diseases of citrus fruits, Asiatic citrus canker. Based on the bacterial host range, the pathogenic forms, A, A*, and Aw have been identified. A*-type strains are not well characterized and they have been reported to differ from other A-type strains on the basis of biochemical and genetic characteristics. In this study, we attempted to develop a medium to mimic in planta conditions for inducing protein secretion of the A*-type strain-NIGEB-088. We also attempted to achieve efficient extraction of Xcc A*-type extracellular proteins, the key mediators of plant-bacterium interaction. Results obtained by this study demonstrate that the m3 (mMM1) can induce Xcc A*-type strains to secret extracellular proteins in amounts detectable by two dimensional gel electrophoresis (2-DE). We also show that Pyrogallol red-molybdate methanol (PRMM) is more effective for precipitation of Xcc A*-type secreted proteins than other assessed procedures of protein precipitation, i. e. acetone, TCA, and sodium deoxycholate | ||
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