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ابراهیم زاده موسوی, حسینعلی, Ebrahimzadeh Mousavi, Hossein Ali, Soltani, Mahdi, Shayan, Parviz, Ebrahimzadeh, Elaheh, Hoseini, Mohammad. (1390). Identification of Gyrodactylus gurleyi in Carassius auratus using morphometric and molecular characterization. , 6(1), 41-46. doi: 10.22059/ijvm.2012.24624
حسینعلی ابراهیم زاده موسوی; Hossein Ali Ebrahimzadeh Mousavi; Mahdi Soltani; Parviz Shayan; Elaheh Ebrahimzadeh; Mohammad Hoseini. "Identification of Gyrodactylus gurleyi in Carassius auratus using morphometric and molecular characterization". , 6, 1, 1390, 41-46. doi: 10.22059/ijvm.2012.24624
ابراهیم زاده موسوی, حسینعلی, Ebrahimzadeh Mousavi, Hossein Ali, Soltani, Mahdi, Shayan, Parviz, Ebrahimzadeh, Elaheh, Hoseini, Mohammad. (1390). 'Identification of Gyrodactylus gurleyi in Carassius auratus using morphometric and molecular characterization', , 6(1), pp. 41-46. doi: 10.22059/ijvm.2012.24624
ابراهیم زاده موسوی, حسینعلی, Ebrahimzadeh Mousavi, Hossein Ali, Soltani, Mahdi, Shayan, Parviz, Ebrahimzadeh, Elaheh, Hoseini, Mohammad. Identification of Gyrodactylus gurleyi in Carassius auratus using morphometric and molecular characterization. , 1390; 6(1): 41-46. doi: 10.22059/ijvm.2012.24624

Identification of Gyrodactylus gurleyi in Carassius auratus using morphometric and molecular characterization

Iranian Journal of Veterinary Medicine
مقاله 8، دوره 6، شماره 1 - شماره پیاپی 848859، خرداد 2012، صفحه 41-46    اصل مقاله (1.27 M)
شناسه دیجیتال (DOI): 10.22059/ijvm.2012.24624
نویسندگان
حسینعلی ابراهیم زاده موسوی1؛ Hossein Ali Ebrahimzadeh Mousavi1؛ Mahdi Soltani1؛ Parviz Shayan2؛ Elaheh Ebrahimzadeh2؛ Mohammad Hoseini3
1گروه بهداشت و بیماریهای آبزیان، دانشکده دامپزشکی، دانشگاه تهران
2گروه انگل شناسی، دانشکده دامپزشکی، دانشگاه تهران
3گروه پاتولوژی، دانشکده دامپزشکی، دانشگاه آزاد اسلامی واحد بابل
چکیده
BACKGROUNDS: Gyrodactylus is a small monogenean ectoparasite that lives on the skin and fins of most of the world's fish species. Gyrodactylus appears to be one of the most prevalent parasites found in ornamental fish, especially in Cyprinids. Goldfish (Carassius auratus) are a popular ornamental fish that are highly contaminated by Gyrodcatylus. OBJECTIVES: The present study is aimed to identify morphological and molecular character-istics of the Gyrodactylus parasite on gold fish. METHODS: The morphological identification of Gyrodactylus specimens was performed using the measurements and drawings of opisthaptoral hard parts of the parasites. The molecular species description was based on a polymerase chain reaction (PCR) of partial sequence of the 5.8S region of ribosomal RNA, and a partial sequence of the internal transcribed spacer 2 (ITS2) of ribosomal RNA. The nucleotide sequences of the PCR products were compared with corresponding sequencing registered in GenBank. RESULTS: Based on the morphometric analysis and sequencing, the Gyrodactylus specimens were described as Gyrodactylus gurleyi. CONCLUSIONS: A combination of molecular techniques with morphological analysis seems to be the best approach for the identification of Gyrodactylus speices.
کلیدواژه‌ها
PCR
آمار
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