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رستمی, امیر, Zahraei Salehi, Taghi, Dehghan, Mohammad Mehdi, Masoudifard, Majid, Memarian, Eeman, Ashrafi Tamai, Eraj. (1390). Salmonella typhimurium in Natrix natrix: detection and identification by culture and multiplex PCR methods. , 5(4), 256-259. doi: 10.22059/ijvm.2011.23582
امیر رستمی; Taghi Zahraei Salehi; Mohammad Mehdi Dehghan; Majid Masoudifard; Eeman Memarian; Eraj Ashrafi Tamai. "Salmonella typhimurium in Natrix natrix: detection and identification by culture and multiplex PCR methods". , 5, 4, 1390, 256-259. doi: 10.22059/ijvm.2011.23582
رستمی, امیر, Zahraei Salehi, Taghi, Dehghan, Mohammad Mehdi, Masoudifard, Majid, Memarian, Eeman, Ashrafi Tamai, Eraj. (1390). 'Salmonella typhimurium in Natrix natrix: detection and identification by culture and multiplex PCR methods', , 5(4), pp. 256-259. doi: 10.22059/ijvm.2011.23582
رستمی, امیر, Zahraei Salehi, Taghi, Dehghan, Mohammad Mehdi, Masoudifard, Majid, Memarian, Eeman, Ashrafi Tamai, Eraj. Salmonella typhimurium in Natrix natrix: detection and identification by culture and multiplex PCR methods. , 1390; 5(4): 256-259. doi: 10.22059/ijvm.2011.23582

Salmonella typhimurium in Natrix natrix: detection and identification by culture and multiplex PCR methods

Iranian Journal of Veterinary Medicine
مقاله 9، دوره 5، شماره 4 - شماره پیاپی 666206، اسفند 2011، صفحه 256-259    اصل مقاله (649.24 K)
شناسه دیجیتال (DOI): 10.22059/ijvm.2011.23582
نویسندگان
امیر رستمی1؛ Taghi Zahraei Salehi2؛ Mohammad Mehdi Dehghan1؛ Majid Masoudifard1؛ Eeman Memarian3؛ Eraj Ashrafi Tamai2
1گروه علوم درمانگاهی، دانشکده دامپزشکی دانشگاه تهران
2گروه میکروبیولوژی، دانشکده دامپزشکی دانشگاه تهران
3دانش‌آموخته دانشکده دامپزشکی دانشگاه تهران
چکیده
A water snake (Natrix natrix) was referred to the Small Animal Teaching Hospital, Faculty of Veterinary Medicine, University of Tehran. It had two subcutaneous masses. Radiographic and ultrasonographic images revealed the presence of two subcutaneous abscesses. The abscesses were removed surgically and specimens were examined by conventional microbial culture and multiplex PCR. Inv-A universal primers were selected for detection of Salmonella at genus level. In order to identify Salmonella tphimurium, specific primers of Rfbj, Fljb and Flic related on genes sequences of O4, H1:i and H2:1,2, respectively, were used. In the positive control for expected size, PCR products were amplified from the fliC, inv-A, fljB and rfbJ genes. According to the results, this study showed antigens can be useful for detecting and identifying Salmonella typhimurium and can be achieved by using specific primers of O4, H1:i and H2:1,2 antigen, because only S. typhimurium has this antigenic structure out of about 2668 Salmonella serovars
کلیدواژه‌ها
Natrix natrix
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