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Amini, Mohammad Sobhan, Akbarinejad, Vahid, Ghasemzadeh-nava, Hamid, Shahverdi, Abdolhossein, Esmaeili, Vahid. (1405). Supplementation of Semen Extender with Mito-TEMPO Could Improve Sperm Motility and Morphology over a 48-Hour Period of Storage in Dareshuri Stallions. , (), -. doi: 10.22059/ijvm.2026.410883.1006046
Mohammad Sobhan Amini; Vahid Akbarinejad; Hamid Ghasemzadeh-nava; Abdolhossein Shahverdi; Vahid Esmaeili. "Supplementation of Semen Extender with Mito-TEMPO Could Improve Sperm Motility and Morphology over a 48-Hour Period of Storage in Dareshuri Stallions". , , , 1405, -. doi: 10.22059/ijvm.2026.410883.1006046
Amini, Mohammad Sobhan, Akbarinejad, Vahid, Ghasemzadeh-nava, Hamid, Shahverdi, Abdolhossein, Esmaeili, Vahid. (1405). 'Supplementation of Semen Extender with Mito-TEMPO Could Improve Sperm Motility and Morphology over a 48-Hour Period of Storage in Dareshuri Stallions', , (), pp. -. doi: 10.22059/ijvm.2026.410883.1006046
Amini, Mohammad Sobhan, Akbarinejad, Vahid, Ghasemzadeh-nava, Hamid, Shahverdi, Abdolhossein, Esmaeili, Vahid. Supplementation of Semen Extender with Mito-TEMPO Could Improve Sperm Motility and Morphology over a 48-Hour Period of Storage in Dareshuri Stallions. , 1405; (): -. doi: 10.22059/ijvm.2026.410883.1006046

Supplementation of Semen Extender with Mito-TEMPO Could Improve Sperm Motility and Morphology over a 48-Hour Period of Storage in Dareshuri Stallions

Iranian Journal of Veterinary Medicine
مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 29 اردیبهشت 1405
نوع مقاله: Original Articles
شناسه دیجیتال (DOI): 10.22059/ijvm.2026.410883.1006046
نویسندگان
Mohammad Sobhan Amini1؛ Vahid Akbarinejad* 2؛ Hamid Ghasemzadeh-nava2؛ Abdolhossein Shahverdi3؛ Vahid Esmaeili4
1Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran
2Department of Theriogenology, Faculty of Veterinary Medicine, University of Tehran
3Department of Embryology, Reproduction Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACER, Tehran, Iran.
4Department of Embryology, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
چکیده
Background: Mito-TEMPO, as a mitochondria-targeted antioxidant, has been reported to improve semen preservation in various species; however, limited information is available on the effect of Mito-TEMPO on storage of equine semen.
Objectives: Accordingly, the current research was designed to investigate the impact of Mito-TEMPO on cooled semen of Dareshuri stallions over a 48-hour storage period.
Methods: In experiment I (n = 7 stallions), the effect of 0 (control group), 25, 50, 100 and 200 μM of Mito-TEMPO was assessed on sperm motility, morphology, viability and plasma membrane integrity at timepoints 0, 24 and 48 hours. In experiment II (n = 4 stallions), the effect of 0 (control group) and 100 μM (the most effective dose found in experiment I) of Mito-TEMPO was evaluated on mitochondrial membrane potential and reactive oxygen species (ROS) at timepoints 0, 24 and 48 hours.
Results: In experiment I, dose 100 μM was recognized as the most effective dose of Mito-TEMPO in equine cooled semen as it tended to improve progressive motility (P = 0.095) and enhanced morphology of sperm (P < 0.05). Other doses of Mito-TEMPO also improved sperm morphology (P < 0.05), but did not influence sperm motility (P > 0.05). Viability and plasma membrane integrity were not affected by different doses of Mito-TEMPO (P > 0.05). In experiment II, however, this effective dose of Mito-TEMPO (100 μM) did not significantly impact mitochondrial membrane potential, proportion of live ROS-positive sperm and proportion of dead ROS-positive sperm motility in equine semen (P > 0.05).
Conclusion: In conclusion, the present study indicated that Mito-TEMPO enhanced preservation of cooled equine semen over a 48-hour period, implying that this mitochondria-targeted component could serve as an effective antioxidant in equine semen extender.
کلیدواژه‌ها
Antioxidant؛ Equine semen preservation؛ Mitochondria؛ Mito-TEMPO؛ Reactive oxygen species
آمار
تعداد مشاهده مقاله: 35





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