پیوندهای مفید
اخبار و اعلانات
آمار
| تعداد نشریات | 127 |
| تعداد شمارهها | 7,196 |
| تعداد مقالات | 77,227 |
| تعداد مشاهده مقاله | 157,208,887 |
| تعداد دریافت فایل اصل مقاله | 118,400,377 |
Nanocurcumin Impact on the Expression of bap Gene | ||
| Iranian Journal of Veterinary Medicine | ||
| مقالات آماده انتشار، پذیرفته شده، انتشار آنلاین از تاریخ 29 اردیبهشت 1405 | ||
| نوع مقاله: Original Articles | ||
| شناسه دیجیتال (DOI): 10.22059/ijvm.2026.406766.1005976 | ||
| نویسندگان | ||
| izdehar A jasim* 1؛ Ghassan A jasim2؛ samar abdulwahab3 | ||
| 1Diyala province, Iraq | ||
| 2.Department of Biology, Faculty of education for pure sciences, University of Diyala, Diyala, Iraq. | ||
| 3Department of Biology, Faculty of sciences, University of Diyala , Diyala, Iraq. | ||
| چکیده | ||
| Background : Acinetobacter baumannii is an opportunistic pathogen known for its multidrug resistance and strong ability to form biofilms, which significantly contributes to its persistence in clinical environments. Objectives: This research aimed to assess the impact of nano-curcumin onto both the antibacterial efficacy and biofilm forming capacity for clinical isolates multidrug-resistant A. baumannii, and to assess the level of expression for bap gene related to the production of biofilms. Methods: the effectiveness of nano curcumin was evaluated in vitro on 19 clinical isolates MDR A. baumannii. the activity of nano-curcumin for preventing A. baumannii biofilm was evaluated using 96-well micro-titer plates. The antibacterial efficacy was assessed by using the broth micro-dilution technique and by estimating MIC. The kirby-bauer disc diffusion method assessed the susceptibility of isolates for different antibiotics.The micro-dilution broth technique determined the mic of nano curcumin. the expression of the bap gene was assessed utilizing qRT-PCR. outside the ten isolates of A baumannii MDR, there can be a strong biofilms producer. Results: Maximum resistance was observed against amoxicillin–clavulanic acid and ceftazidime, with all 19 isolates (100%) showing resistance to both antibiotics. In contrast, minimum resistance was recorded for meropenem, with only four isolates (21.05%) exhibiting resistance. Ten isolates demonstrated a significant capacity for biofilm formation. The minimum inhibitory concentration (MIC) of nanocurcumin was 128 µg/mL for all ten biofilm-forming isolates. Furthermore, treatment with nanocurcumin resulted in a decrease of bap gene expression to 0.35-fold compared with untreated samples. Conclusion: These results indicate that nanocurcumin may serve as a potential agent for reducing the development of Acinetobacter biofilms. The observed decreased levels of the bap gene further corroborate the anti-biofilm efficacy of nanocurcumin. | ||
| کلیدواژهها | ||
| Acinetobacter baumannii؛ bap gene؛ biofilm؛ multidrug-resistance؛ nanocurcumin | ||
|
آمار تعداد مشاهده مقاله: 95 |
||
سامانه مدیریت نشریات علمی. قدرت گرفته از سیناوب