| تعداد نشریات | 127 |
| تعداد شمارهها | 7,147 |
| تعداد مقالات | 76,904 |
| تعداد مشاهده مقاله | 154,931,991 |
| تعداد دریافت فایل اصل مقاله | 116,905,379 |
افزایش ماندگاری Trichoderma harzianum در حالت کپسوله به عنوان یک راهبرد مهار زیستی علیه قارچ خاکزی Sclerotinia sclerotiorum در شرایط درونشیشهای و گلخانه | ||
| دانش گیاهپزشکی ایران | ||
| دوره 56، شماره 1، تیر 1404، صفحه 179-200 اصل مقاله (2 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijpps.2026.409909.1007107 | ||
| نویسندگان | ||
| عبدالحسین طاهری* 1؛ الهه لطفعلی نژاد2؛ سیدجواد صانعی1؛ زهرا شهرآبادی3 | ||
| 1گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| 2گروه گیاهپزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| 3بخش تحقیقات شرکت دانازیست لوتوس، گرگان، ایران | ||
| چکیده | ||
| در سالهای اخیر، افزایش نگرانیها نسبت به اثرات زیستمحیطی و بهداشتی سموم شیمیایی، بر ضرورت فوری مدیریت پایدار بیماریهای گیاهی تاکید کرده است. لذا این پژوهش با هدف ارزیابی تاثیر فرمولاسیون کپسول حاوی Trichoderma harzianum بر مهار Sclerotinia sclerotiorum در گیاه گوجهفرنگی انجام شد. کپسولها با استفاده از روش ژلاسیون یونی و آلژینات سدیم تهیه گردید و ویژگیهای فیزیکی، میزان بارگذاری و آزادسازی اسپور، نرخ تورم، پایداری در برابر دما و اشعه فرابنفش و نیز زیستتخریبپذیری کپسولها مورد بررسی قرار گرفت. نتایج نشان داد فرمولاسیون کپسوله به طور مؤثری موجب افزایش ماندگاری و زندهمانی اسپورهای T. harzianum و حفظ فعالیت زیستی آنها در مقایسه با فرم غیرکپسوله شدند. درصد ماندگاری اسپورهای T. harzianum غیرکپسوله پس از ۹۰ روز در دماهای ۲۵ و ۳۵ درجه بهشدت کاهش یافت. در حالی که اسپورهای کپسولهشده در همان شرایط ۴۲ و ۴۶ درصد کاهش بقا نشان دادند. در بررسی گلخانهای نیز درصد بیماری در گیاهان تیمار شده با T. harzianum کپسولهشده 4۴/4 درصد و در شاهد 3۳/73 درصد بود. فرمولاسیون کپسوله نسبت به حالت غیرکپسوله اثربخشی بیشتری در کاهش بیماری داشت. سطح آنزیمهای دفاعی در گیاهان تیمار شده با T. harzianum کپسوله نسبت به T. harzianum غیرکپسوله و شاهد به ترتیب 32/10- 25/14 و 27/11-66/31 درصد افزایش داشت. یافتههای این پژوهش ضمن تأکید بر نقش مؤثر و پایدار فرمولاسیون کپسوله در بهبود عملکرد عوامل زیستی، نشاندهنده قابلیت بالای این فناوری در کاهش خسارت ناشی از S. sclerotiorum و ارتقای سلامت گیاه در سیستمهای کشاورزی میباشد. | ||
| کلیدواژهها | ||
| Trichoderma harzianum؛ فرمولاسیون کپسول؛ کنترل زیستی؛ گلخانه؛ ماندگاری solani | ||
| مراجع | ||
منابعرضوی، س.ا.، صانعی، س.ج.، شربتخواری، م.و، و قربانی نصرآبادی، ر. 1400. ردیابی و جداسازی قارچها و شبهقارچهای خاک. انتشارات پیک ریحان گرگان، 384 صفحه. صانعی، س.ج.، و رضوی، س.ا. 1398. تاثیر برخی از گونههای قارچ تریکودرما بر رشد گیاه دارویی ریحان (Ocimum basilicum L.) و پاسخهای دفاعی گیاه علیه Rhizoctonia solan. کنترل بیولوژیک آفات و بیماریهای گیاهی، 8 (2): 27-38. 10.22059/jbioc.2018.238913.208
REFERENCES Abbas, M. M., Ismael, W. H., Mahfouz, A. Y., Daigham, G. E., & Attia, M. S. (2024). Efficacy of endophytic bacteria as promising inducers for enhancing the immune responses in tomato plants and managing Rhizoctonia root-rot disease. Scientific Reports, 14(1), 1331. https://doi.org/10.1038/s41598-023-51000-8 Ab Rahman, S. F. S., Singh, E., Pieterse, C. M., & Schenk, P. M. (2018). Emerging microbial biocontrol strategies for plant pathogens. Plant Science, 267, 102-111. https://doi.org/10.1016/j.plantsci.2017.11.012 Acuña-Jiménez, M., Rosas-García, N. M., López-Meyer, M., Saínz-Hernández, J. C., Mundo-Ocampo, M., & García-Gutiérrez, C. (2015). Pathogenicity of microencapsulated insecticide from Beauveria bassiana and Metarhizium anisopliae against tobacco budworm, Heliothis virescens (Fabricius). Southwestern Entomologist, 40(3), 531-538. doi.org/10.3958/059.040.0311 Adzmi, F., Musa, M. H., Siddiqui, Y., Yun, W. M., Hamid, H. A., Abdu, A., & Abiri, R. (2021). Development of alginate-montmorillonite-starch with encapsulated Trichoderma harzianum and evaluation of conidia shelf life. doi:10.17957/IJAB/15.1812 Almeida, F., Rodrigues, M. L., & Coelho, C. (2019). The still underestimated problem of fungal diseases worldwide. Frontiers in microbiology, 10, 214. https://doi.org/10.3389/fmicb.2019.00214 Arias-Chavarría, L. D., Batista-Menezes, D., Orozco-Cayasso, S., Vargas-Martínez, A., Vega-Baudrit, J. R., & Montes de Oca-Vásquez, G. (2025). Evaluation of the viability of microencapsulated Trichoderma longibrachiatum conidia as a strategy to prolong the shelf life of the fungus as a biological control agent. Frontiers in Chemistry, 12, 1473217. https://doi.org/10.3389/fchem.2024.1473217 Atlagić, K., Cvetić Antić, T., Lukičić, J., Kruščić, K., Živić, M., Unković, N., ... & Todorović, N. V. (2025). Biocontrol Potential of Native Trichoderma Strains Toward Soil-Borne Phytopathogenic and Saprotrophic Fungi. Journal of Fungi, 11(7), 535. https://doi.org/10.3390/jof11070535 Balla, A., Silini, A., Cherif-Silini, H., Chenari Bouket, A., Alenezi, F. N., & Belbahri, L. (2022). Recent advances in encapsulation techniques of plant growth-promoting microorganisms and their prospects in the sustainable agriculture. Applied Sciences, 12(18), 9020. https://doi.org/10.3390/app12189020 Bhai, R. (2020). Preservation and long-term storage of Trichoderma spp. by sodium alginate encapsulation. Journal of Plantation Crops, 48(1), 36-44. https://doi.org/10.25081/jpc.2020.v48.i1.6215 Calabi-Floody, M., Medina, J., Rumpel, C., Condron, L. M., Hernandez, M., Dumont, M., & de La Luz Mora, M. (2018). Smart fertilizers as a strategy for sustainable agriculture. Advances in Agronomy, 147, 119-157. https://doi.org/10.1016/bs.agron.2017.10.003 Dennis, C., & Webster, J. (1971a). Antagonistic properties of species-groups of Trichoderma: I. Production of non-volatile antibiotics. Transactions of the British Mycological Society, 57(1), 25-IN3. https://doi.org/10.1016/S0007-1536(71)80077-3 Dennis, C., & Webster, J. (1971b). Antagonistic properties of species-groups of Trichoderma: II. Production of volatile antibiotics. Transactions of the British Mycological Society, 57: 41-48. https://doi.org/10.1016/S0007-1536(71)80078-5 Dennis, C. and Webster, J. 1971c. Antagonistic properties of species – groups of Trichoderma III. Hyphal interactions. Transactions of the British Mycological Society. 57: 363-369. https://doi.org/10.1016/S0007-1536(71)80050-5 Dos Santos, G. F., Locatelli, G. O., Coêlho, D. A., Botelho, P. S., De Amorim, M. S., De Vasconcelos, T. C. L., & Bueno, L. A. (2015). Factorial design, preparation and characterization of new beads formed from alginate, polyphosphate and glycerol gelling solution for microorganism microencapsulation. Journal of Sol-Gel Science and Technology, 75(2), 345-352. https://doi.org/10.1007/s10971-015-3705-5 Filizola, P. R. B., Luna, M. A. C., de Souza, A. F., Coelho, I. L., Laranjeira, D., & Campos-Takaki, G. M. (2019). Biodiversity and phylogeny of novel Trichoderma isolates from mangrove sediments and potential of biocontrol against Fusarium strains. Microbial cell factories, 18(1), 89. https://doi.org/10.1186/s12934-019-1108-y Hermosa, R., Viterbo, A., Chet, I., & Monte, E. (2012). Plant-beneficial effects of Trichoderma and of its genes. Microbiology, 158(1), 17-25. https://doi.org/10.1099/mic.0.052274-0 Hossain, M. M., Sultana, F., Li, W., Tran, L. S. P., & Mostofa, M. G. (2023). Sclerotinia sclerotiorum (Lib.) de Bary: Insights into the pathogenomic features of a global pathogen. Cells, 12(7), 1063. https://doi.org/10.3390/cells12071063 Huang, W., Wang, Y., Ren, L., Du, C., & Shi, X. (2009). A novel PHBV/HA microsphere releasing system loaded with alendronate. Materials Science and Engineering: C, 29(7), 2221-2225. https://doi.org/10.1016/j.msec.2009.05.015 Jain, A., Sarsaiya, S., Wu, Q., Lu, Y., & Shi, J. (2019). A review of plant leaf fungal diseases and its environment speciation. Bioengineered, 10(1), 409-424. https://doi.org/10.1080/21655979.2019.1649520 Jing, H. U., Zuobing, X. I. A. O., Rujun, Z. H. O. U., Shuangshuang, M. A., Mingxi, W. A. N. G., & Zhen, L. I. (2011). Properties of aroma sustained-release cotton fabric with rose fragrance nanocapsule. Chinese Journal of Chemical Engineering, 19(3), 523-528. https://doi.org/10.1016/S1004-9541(11)60016-5 Jurić, S., Đermić, E., Topolovec-Pintarić, S., Bedek, M., & Vinceković, M. (2019). Physicochemical properties and release characteristics of calcium alginate microspheres loaded with Trichoderma viride spores. Journal of Integrative Agriculture, 18(11), 2534-2548. https://doi.org/10.1016/S2095-3119(19)62634-1 Karthikeyan, M., Radhika, K., Mathiyazhagan, S., Bhaskaran, R., Samiyappan, R., & Velazhahan, R. (2006). Induction of phenolics and defense-related enzymes in coconut (Cocos nucifera L.) roots treated with biocontrol agents. Brazilian Journal of Plant Physiology, 18, 367. DOI:10.1590/S1677-04202006000300003 Khedher, S. B., Mejdoub-Trabelsi, B., & Tounsi, S. (2021). Biological potential of Bacillus subtilis V26 for the control of Fusarium wilt and tuber dry rot on potato caused by Fusarium species and the promotion of plant growth. Biological Control, 152, 104444. https://doi.org/10.1016/j.biocontrol.2020.104444 Khalil, A. M. A., Hashem, A. H., & Abdelaziz, A. M. (2019). Occurrence of toxigenic Penicillium polonicum in retail green table olives from the Saudi Arabia market. Biocatalysis and Agricultural Biotechnology, 21, 101314. DOI:10.1016/j.bcab.2019.101314 Leong, J. Y., Lam, W. H., Ho, K. W., Voo, W. P., Lee, M. F. X., Lim, H. P., ... & Chan, E. S. (2016). Advances in fabricating spherical alginate hydrogels with controlled particle designs by ionotropic gelation as encapsulation systems. Particuology, 24, 44-60. https://doi.org/10.1016/j.partic.2015.09.004 Liu, C. P., & Liu, S. D. (2009). Formulation and characterization of the microencapsulated entomopathogenic fungus Metarhizium anisopliae MA126. Journal of Microencapsulation, 26(5), 377-384. https://doi.org/10.1080/02652040802365455 Locatelli, G. O., dos Santos, G. F., Botelho, P. S., Finkler, C. L. L., & Bueno, L. A. (2018). Development of Trichoderma sp. formulations in encapsulated granules (CG) and evaluation of conidia shelf-life. Biological Control, 117, 21-29. https://doi.org/10.1016/j.biocontrol.2017.08.020 Lopes, M. M., de Oliveira-Paiva, C. A., & Farinas, C. S. (2023). Modification of pectin/starch-based beads with additives to improve Bacillus subtilis encapsulation for agricultural applications. International Journal of Biological Macromolecules, 246, 125646. https://doi.org/10.1016/j.ijbiomac.2023.125646 Lotfalinezhad, E., Taheri, A., Razavi, S. E., & Sanei, S. J. (2023). Preparation of Bacillus subtilis capsule formulation and evaluation of its efficacy in controlling damping-off in tomato caused by Rhizoctonia solani under greenhouse conditions. BioControl in Plant Protection, 10(2), 125-139. Doi: 10.22092/bcpp.2023.363355.344 Lotfalinezhad, E., Taheri, A., Razavi, S. E., & Sanei, S. J. (2024). Preparation and assessment of alginate-microencapsulated Trichoderma harzianum for controlling Sclerotinia sclerotiorum and Rhizoctonia solani on tomato. International Journal of Biological Macromolecules, 259, 129278. https://doi.org/10.1016/j.ijbiomac.2024.129278 Maestrelli, F., Zerrouk, N., Cirri, M., Mennini, N., & Mura, P. (2008). Microspheres for colonic delivery of ketoprofen-hydroxypropyl-β-cyclodextrin complex. european journal of pharmaceutical sciences, 34(1), 1-11. https://doi.org/10.1016/j.ejps.2008.02.001 Mancera-López, M. E., Izquierdo-Estévez, W. F., Escalante-Sánchez, A., Ibarra, J. E., & Barrera-Cortés, J. (2019). Encapsulation of Trichoderma harzianum conidia as a method of conidia preservation at room temperature and propagation in submerged culture. Biocontrol Science and Technology, 29(2), 107-130. https://doi.org/10.1080/09583157.2018.1535053 Manoharachary, C., Singh, H. B., & Varma, A. (Eds.). (2020). Trichoderma: agricultural applications and beyond (pp. 87-112). Cham, Switzerland: Springer. https://doi.org/10.1007/978-3-030-54758-5 Maruyama, C. R., Bilesky-José, N., de Lima, R., & Fraceto, L. F. (2020). Encapsulation of Trichoderma harzianum preserves enzymatic activity and enhances the potential for biological control. Frontiers in Bioengineering and Biotechnology, 8, 225. https://doi.org/10.3389/fbioe.2020.00225 Martínez-Cano, B., Mendoza-Meneses, C. J., García-Trejo, J. F., Macías-Bobadilla, G., Aguirre-Becerra, H., Soto-Zarazúa, G. M., & Feregrino-Pérez, A. A. (2022). Review and perspectives of the use of alginate as a polymer matrix for microorganisms applied in agro-industry. Molecules, 27(13), 4248. https://doi.org/10.3390/molecules27134248 Mazzola, M., & Freilich, S. (2017). Prospects for biological soilborne disease control: application of indigenous versus synthetic microbiomes. Phytopathology, 107(3), 256-263. https://doi.org/10.1094/PHYTO-09-16-0330-RVW Moradi Pour, M., Saberi Riseh, R., & Skorik, Y. A. (2022). Sodium alginate–gelatin nanoformulations for encapsulation of Bacillus velezensis and their use for biological control of pistachio gummosis. Materials, 15(6), 2114. https://doi.org/10.3390/ma15062114 Muñoz-Celaya, A. L., Ortiz-García, M., Vernon-Carter, E. J., Jauregui-Rincón, J., Galindo, E., & Serrano-Carreón, L. (2012). Spray-drying microencapsulation of Trichoderma harzianum conidias in carbohydrate polymers matrices. Carbohydrate polymers, 88(4), 1141-1148. https://doi.org/10.1016/j.carbpol.2011.12.030 Naraghi, L., & Nattaj, M. R. (2022). Efficacy of Talaromyces flavus microcapsule in controlling cotton important fungal diseases. Revista De Gestao Social E Ambiental, 16(2), e03023-e03023. https://doi.org/10.24857/rgsa.v16n2-026 O’Sullivan, C. A., Belt, K., & Thatcher, L. F. (2021). Tackling control of a cosmopolitan phytopathogen: Sclerotinia. Frontiers in Plant Science, 12, 707509. https://doi.org/10.3389/fpls.2021.707509 Poletto, F. S., Jäger, E., Cruz, L., Pohlmann, A. R., & Guterres, S. S. (2008). The effect of polymeric wall on the permeability of drug-loaded nanocapsules. Materials Science and Engineering: C, 28(4), 472-478. https://doi.org/10.1016/j.msec.2007.04.015 Pour, M. M., Saberi-Riseh, R., Mohammadinejad, R., & Hosseini, A. (2019). Investigating the formulation of alginate-gelatin encapsulated Pseudomonas fluorescens (VUPF5 and T17-4 strains) for controlling Fusarium solani on potato. International Journal of Biological Macromolecules, 133, 603-613. https://doi.org/10.1016/j.ijbiomac.2019.04.071 Pour, M. M., Hassanisaadi, M., Kennedy, J. F., & Riseh, R. S. (2024). A novel biopolymer technique for encapsulation of Bacillus velezensis BV9 into double coating biopolymer made by in alginate and natural gums to biocontrol of wheat take-all disease. International Journal of Biological Macromolecules, 257, 128526. https://doi.org/10.1016/j.ijbiomac.2023.128526 Przyklenk, M., Vemmer, M., Hanitzsch, M., & Patel, A. (2017). A bioencapsulation and drying method increases shelf life and efficacy of Metarhizium brunneum conidia. Journal of microencapsulation, 34(5), 498-512. https://doi.org/10.1080/02652048.2017.1354941 Qi, Q., Fan, C., Wu, H., Sun, L., & Cao, C. (2023). Preparation of Trichoderma asperellum microcapsules and biocontrol of cucumber powdery mildew. Microbiology Spectrum, 11(3), e05084-22. https://doi.org/10.1128/spectrum.05084-22 Rodrigues, S., Da Costa, A. M. R., & Grenha, A. (2012). Chitosan/carrageenan nanoparticles: Effect of cross-linking with tripolyphosphate and charge ratios. Carbohydrate polymers, 89(1), 282-289. https://doi.org/10.1016/j.carbpol.2012.03.010 Rodrigues, F. J., Cedran, M. F., Bicas, J. L., & Sato, H. H. (2020). Encapsulated probiotic cells: Relevant techniques, natural sources as encapsulating materials and food applications–A narrative review. Food Research International, 137, 109682. https://doi.org/10.1016/j.foodres.2020.109682 Saberi‐Riseh, R., & Moradi‐Pour, M. (2021). A novel encapsulation of Streptomyces fulvissimus Uts22 by spray drying and its biocontrol efficiency against Gaeumannomyces graminis, the causal agent of take‐all disease in wheat. Pest Management Science, 77(10), 4357-4364. https://doi.org/10.1002/ps.6469 Salunke, P., & Koche, D. (2023). Role of phenolic compounds in plant defense mechanism: an updated review. Indian Journal of Applied Pure Bio, 38(3), 1199-1215. ISSN: 0970-2091 Saharan, G. S., & Mehta, N. (2008). Sclerotinia diseases of crop plants: biology, ecology and disease management. Dordrecht: Springer Netherlands. https://doi.org/10.1007/978-1-4020-8408-9 Sharma, S., Kaur, I., & Nagpal, A. K. (2024). Pesticides in agriculture: Food security vs. food safety. In Advances in Food Security and Sustainability (Vol. 9, pp. 59-73). Elsevier. https://doi.org/10.1016/bs.af2s.2024.07.010 Shiade, S. R. G., Zand-Silakhoor, A., Fathi, A., Rahimi, R., Minkina, T., Rajput, V. D., … & Chaudhary, T. (2024). Plant metabolites and signaling pathways in response to biotic and abiotic stresses: Exploring bio stimulant applications. Plant Stress, 12, 100454. https://doi.org/10.1016/j.stress.2024.100454 Tu, L., He, Y., Yang, H., Wu, Z., & Yi, L. (2015). Preparation and characterization of alginate–gelatin microencapsulated Bacillus subtilis SL-13 by emulsification/internal gelation. Journal of Biomaterials Science, Polymer Edition, 26(12), 735-749. https://doi.org/10.1080/09205063.2015.1056075 Vemmer, M., & Patel, A. V. (2013). Review of encapsulation methods suitable for microbial biological control agents. Biological Control, 67(3), 380-389. https://doi.org/10.1016/j.biocontrol.2013.09.003 Vessey, J. K. (2003). Plant growth promoting rhizobacteria as biofertilizers. Plant and Soil, 255(2), 571-586. https://doi.org/10.1023/A:1026037216893 Vindas-Reyes, E., Chacón-Cerdas, R., & Rivera-Méndez, W. (2024). Trichoderma production and encapsulation methods for agricultural applications. AgriEngineering, 6(3), 2366-2384. https://doi.org/10.3390/agriengineering6030138 Wu, Z., He, Y., Chen, L., Han, Y., & Li, C. (2014). Characterization of Raoultella planticola Rs-2 microcapsule prepared with a blend of alginate and starch and its release behavior. Carbohydrate Polymers, 110, 259-267. https://doi.org/10.1016/j.carbpol.2014.04.011 Xu, L., & Wang, X. (2025). A Comprehensive Review of Phenolic Compounds in Horticultural Plants. International Journal of Molecular Sciences, 26(12), 5767. https://doi.org/10.3390/ijms26125767 | ||
|
آمار تعداد مشاهده مقاله: 129 تعداد دریافت فایل اصل مقاله: 86 |
||