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تاثیر پوششدهی باکتری Bacillus velezensis توسط آلژینات بر شاخصهای رشد و بهبود مقاومت به بوتهمیری رایزوکتونیایی در گیاه گوجهفرنگی | ||
| دانش گیاهپزشکی ایران | ||
| دوره 56، شماره 1، تیر 1404، صفحه 169-178 اصل مقاله (1.48 M) | ||
| نوع مقاله: مقاله پژوهشی | ||
| شناسه دیجیتال (DOI): 10.22059/ijpps.2026.409908.1007106 | ||
| نویسندگان | ||
| عبدالحسین طاهری* 1؛ سیدجواد صانعی1؛ الهه لطفعلی نژاد2؛ امین رادمرد2 | ||
| 1گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| 2دانش آموخته مقطع دکتری رشته بیماری شناسی گیاهی، گروه گیاه پزشکی، دانشکده تولید گیاهی، دانشگاه علوم کشاورزی و منابع طبیعی گرگان، ایران | ||
| چکیده | ||
| این مطالعه با هدف بررسی پایداری عامل مهار زیستی و توسعه فرمولاسیون مناسب جهت افزایش زندهمانی و بهبود عملکرد باکتری Bacillus velezensis انجام شد. در این بررسی تاثیر فرمولاسیون کپسول B. velezensis بر رشد گیاه گوجهفرنگی و پاسخهای دفاعی گیاه علیه Rhizoctonia solani بررسی شد. تهیه کپسولها با استفاده از روش ژلاسیون یونی بود. بذر گوجهفرنگی پس از تیمار با فرمولاسیون کپسوله و غیرکپسوله B. velezensis، در خاک آلوده به R. solani کشت گردید و پس از 40 روز، شاخصهای رشدی گیاه، درصد بیماری مرگ گیاهچه و تغییر فعالیت آنتیاکسیدانی بررسی شد. آزمایش بهصورت فاکتوریل در قالب طرح کاملا تصادفی با چهار تکرار طراحی گردید. درصد زندهمانی B. velezensis کپسوله بعد از 90 روز قرارگیری در دماهای 25 و 35 درجه سلسیوس به ترتیب 58 و 53 درصد بوده است که نقش مثبت کپسولها در افزایش زندهمانی و مقاومت B. velezensis نسبت به حالت غیرکپسول را نشان میداد. کاربرد فرمولاسیون کپسول B. velezensis در شرایط گلخانه کاهش درصد بیماری را در مقایسه با فرمولاسیون غیرکپسول نشان داد. تفاوت معنیداری از نظر شاخصهای رشد در گیاهان تیمار شده با B. velezensis کپسوله در مقایسه با شاهد مشاهده شد و بیشترین تغییرات به ارتفاع ریشه و ساقه مربوط بود. این نتایج بر تاثیر مثبت B. velezensis، همچنین فرمولاسیون کپسول آن در القای مقاومت علیه بیماری مرگ گیاهچه و افزایش شاخصهای رشدی در گیاه گوجهفرنگی تاکید دارد و میتواند بهعنوان یک رهیافت زیستفناور برای بهبود رشد و مقاومت به تنش ناشی از R. solani پیشنهاد شود. | ||
| کلیدواژهها | ||
| القای مقاومت؛ بهبود رشد؛ Bacillus velezensis؛ فرمولاسیون کپسول؛ Rhizoctonia solani | ||
| مراجع | ||
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منابع رضوی، س.ا.، صانعی، س.ج.، شربتخواری، م.و، و قربانی نصرآبادی، ر. 1400. ردیابی و جداسازی قارچها و شبهقارچهای خاک. انتشارات پیک ریحان گرگان، 384 صفحه.
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